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Year 2023, Volume: 44 Issue: 4, 716 - 722, 28.12.2023

Halil İbrahim Ulusoy , İpek Nur Yiğit , Ümmügülsüm Polat , Esra Durgun , Aslıhan Gürbüzer , Songül Ulusoy

https://doi.org/10.17776/csj.1342960

Abstract

References

  • [1] Brancaccio M., Mennitti C., Cesaro A., Fimiani F., Vano M., Gargiulo B., Caiazza M., Amodio F., Coto I., D’alicandro G., Mazzaccara C., Lombardo B., Pero R., Terracciano D., Limongelli G., Calabrò P., D’argenio V., Frisso G., Scudiero O., The Biological Role of Vitamins in Athletes’ Muscle, Heart and Microbiota, Int. J. Environ. Res. Public Health., 19 (2022) 1249.
  • [2] Hashim N.H., Osman R., Abidin N.A.Z., N.S.A. Kassim N.A.Z., Recent trends in the quantification of vitamin b, Malaysian J. Anal. Sci., 25 (2021) 466–482.
  • [3] Hassan O., M.J. Chee, Sensitivity of UV detection in simultaneous separation and detection of B-vitamins using HPLC, Malaysian J. Anal. Sci. 7 (2001) 251–255.
  • [4] Chan Y., Bailey R., O’Connor D.B., Advances in Nutrition, Folate 1, (2013) 123–125.
  • [5] Aygun B., Açık Y.D., Vitamin B12 and Folic Acid Levels After Iron Therapy in Iron-Deficiency Anemia, Cukurova Anestezi ve Cerrahi Bilim. Derg., 3 (2020) 261–267.
  • [6] Ulusoy S., Erdoğan S., Karaslan M. G., Ateş B., Ulusoy H. İ. & Erdemoğlu S. Optimization Of Extraction Parameters For Folic Acid And Antioxidant Compounds From An Edible Plant (Polygonum Cognatum Meissn) Using Pressurized Liquid Extraction (PLE) System, Cumhuriyet Sci. J., 39 (2018) 1069–1080.
  • [7] Smith A. D., Warren M. J.,Refsum, H. Vitamin B12, Adv. Food Nutr. Res., 83 (2018) 215–279.
  • [8] Osman D., Cooke A., Young T. R., Deery E., Robinson N. J., Warren M. J. The requirement for cobalt in vitamin B12: A paradigm for protein metalation, Biochim. Biophys. Acta - Mol. Cell Res., 1868 (2021) 118896.
  • [9] Ulusoy S., Ulusoy H.İ., Preconcentration and determination of safranine T in environmental water samples, Environ. Eng. Manag. J., 17 (2018) 147–154.
  • [10] Halko R., Hagarová I., Andruch V. Innovative approaches in cloud-point extraction, J. Chromatogr. A. 1701 (2023) 464053.
  • [11] Bezerra M. D. A., Arruda M. A. Z. and Ferreira S. L. Cloud point extraction as a procedure of separation and pre‐concentration for metal determination using spectroanalytical techniques: a review, Applied Spectroscopy Reviews, 40 (4) (2005) 269-299.
  • [12] Karaca E., Ulusoy S., Morgül U., Ulusoy H.I. Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction, Cumhuriyet Sci. J., 41 (2020) 826–831.
  • [13] Pragst F. Application of solid-phase microextraction in analytical toxicology, Anal. Bioanal. Chem., 388 (2007) 1393–1414.
  • [14] Ulusoy H.I., Yilmaz Ö., Gürkan R. A micellar improved method for trace levels selenium quantification in food samples, alcoholic and nonalcoholic beverages through CPE/FAAS, Food Chem., 139 (2013) 1008–1014.
  • [15] Ulusoy H.İ., Acıdereli H., Ulusoy S., Erdoğan S. Development of a New Methodology for Determination of Vitamin B9 at Trace Levels by Ultrasonic-Assisted Cloud Point Extraction Prior to HPLC, Food Anal. Methods, 10 (2017) 799–808.
  • [16] Ulusoy S., Akçay M. Simultaneous Determination of Vitamins B1 and B2 in Food Samples by Modified Cloud Point Extraction Method and HPLC-DAD, Food Anal. Methods, 11 (2018) 260–269.
  • [17] Kori S. Cloud point extraction coupled with back extraction: a green methodology in analytical chemistry, Forensic Sci. Res., 6 (2021) 19–33.
  • [18] Kojro, G., Rudzki, P. J., Pisklak, D. M. and Giebułtowicz, J. Matrix effect screening for cloud-point extraction combined with liquid chromatography coupled to mass spectrometry: bioanalysis of pharmaceuticals, Journal of Chromatography A, 1591 (2019) 44-54.

Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction

Year 2023, Volume: 44 Issue: 4, 716 - 722, 28.12.2023

Halil İbrahim Ulusoy , İpek Nur Yiğit , Ümmügülsüm Polat , Esra Durgun , Aslıhan Gürbüzer , Songül Ulusoy

https://doi.org/10.17776/csj.1342960

Abstract

An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the HPLC analysis. All parameters affecting the CPE method such as pH, buffer volume, incubation time, surfactant and electrolyte concentration, solvent for the surfactant-rich phase and its amount have been individually studied and optimized step by step. After the optimization of all parameters of the CPE process, the detection limits of the developed method for B1, B9 and B12 vitamins were calculated as 1.42 ng mL-1, 7.14 ng mL-1 and 14.28 ng mL-1 , respectively. The linear working ranges for three vitamin molecules was obtained in the range of 5.0-500.0 ng mL-1. After CPE procedure, determination of vitamin molecules was carried out by using HPLC system with diode array detector(DAD) at 244 nm for vitamin B1, 285 nm for vitamin B9, and 361 nm for vitamin B12, respectively

Keywords

Cloud point extraction HPLC Vitamin B1 Vitamin B9 Vitamin B12

References

  • [1] Brancaccio M., Mennitti C., Cesaro A., Fimiani F., Vano M., Gargiulo B., Caiazza M., Amodio F., Coto I., D’alicandro G., Mazzaccara C., Lombardo B., Pero R., Terracciano D., Limongelli G., Calabrò P., D’argenio V., Frisso G., Scudiero O., The Biological Role of Vitamins in Athletes’ Muscle, Heart and Microbiota, Int. J. Environ. Res. Public Health., 19 (2022) 1249.
  • [2] Hashim N.H., Osman R., Abidin N.A.Z., N.S.A. Kassim N.A.Z., Recent trends in the quantification of vitamin b, Malaysian J. Anal. Sci., 25 (2021) 466–482.
  • [3] Hassan O., M.J. Chee, Sensitivity of UV detection in simultaneous separation and detection of B-vitamins using HPLC, Malaysian J. Anal. Sci. 7 (2001) 251–255.
  • [4] Chan Y., Bailey R., O’Connor D.B., Advances in Nutrition, Folate 1, (2013) 123–125.
  • [5] Aygun B., Açık Y.D., Vitamin B12 and Folic Acid Levels After Iron Therapy in Iron-Deficiency Anemia, Cukurova Anestezi ve Cerrahi Bilim. Derg., 3 (2020) 261–267.
  • [6] Ulusoy S., Erdoğan S., Karaslan M. G., Ateş B., Ulusoy H. İ. & Erdemoğlu S. Optimization Of Extraction Parameters For Folic Acid And Antioxidant Compounds From An Edible Plant (Polygonum Cognatum Meissn) Using Pressurized Liquid Extraction (PLE) System, Cumhuriyet Sci. J., 39 (2018) 1069–1080.
  • [7] Smith A. D., Warren M. J.,Refsum, H. Vitamin B12, Adv. Food Nutr. Res., 83 (2018) 215–279.
  • [8] Osman D., Cooke A., Young T. R., Deery E., Robinson N. J., Warren M. J. The requirement for cobalt in vitamin B12: A paradigm for protein metalation, Biochim. Biophys. Acta - Mol. Cell Res., 1868 (2021) 118896.
  • [9] Ulusoy S., Ulusoy H.İ., Preconcentration and determination of safranine T in environmental water samples, Environ. Eng. Manag. J., 17 (2018) 147–154.
  • [10] Halko R., Hagarová I., Andruch V. Innovative approaches in cloud-point extraction, J. Chromatogr. A. 1701 (2023) 464053.
  • [11] Bezerra M. D. A., Arruda M. A. Z. and Ferreira S. L. Cloud point extraction as a procedure of separation and pre‐concentration for metal determination using spectroanalytical techniques: a review, Applied Spectroscopy Reviews, 40 (4) (2005) 269-299.
  • [12] Karaca E., Ulusoy S., Morgül U., Ulusoy H.I. Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction, Cumhuriyet Sci. J., 41 (2020) 826–831.
  • [13] Pragst F. Application of solid-phase microextraction in analytical toxicology, Anal. Bioanal. Chem., 388 (2007) 1393–1414.
  • [14] Ulusoy H.I., Yilmaz Ö., Gürkan R. A micellar improved method for trace levels selenium quantification in food samples, alcoholic and nonalcoholic beverages through CPE/FAAS, Food Chem., 139 (2013) 1008–1014.
  • [15] Ulusoy H.İ., Acıdereli H., Ulusoy S., Erdoğan S. Development of a New Methodology for Determination of Vitamin B9 at Trace Levels by Ultrasonic-Assisted Cloud Point Extraction Prior to HPLC, Food Anal. Methods, 10 (2017) 799–808.
  • [16] Ulusoy S., Akçay M. Simultaneous Determination of Vitamins B1 and B2 in Food Samples by Modified Cloud Point Extraction Method and HPLC-DAD, Food Anal. Methods, 11 (2018) 260–269.
  • [17] Kori S. Cloud point extraction coupled with back extraction: a green methodology in analytical chemistry, Forensic Sci. Res., 6 (2021) 19–33.
  • [18] Kojro, G., Rudzki, P. J., Pisklak, D. M. and Giebułtowicz, J. Matrix effect screening for cloud-point extraction combined with liquid chromatography coupled to mass spectrometry: bioanalysis of pharmaceuticals, Journal of Chromatography A, 1591 (2019) 44-54.
There are 18 citations in total.

Details

Primary Language English
Subjects Bioassays, Instrumental Methods, Analytical Chemistry (Other)
Journal Section Natural Sciences
Authors

Halil İbrahim Ulusoy 0000-0002-8742-5145

İpek Nur Yiğit 0009-0009-0593-5394

Ümmügülsüm Polat 0000-0001-8393-2396

Esra Durgun 0009-0009-2613-4019

Aslıhan Gürbüzer 0000-0002-6753-2808

Songül Ulusoy 0000-0003-3640-5416

Publication Date December 28, 2023
Submission Date August 16, 2023
Acceptance Date November 30, 2023
Published in Issue Year 2023Volume: 44 Issue: 4

Cite

APA Ulusoy, H. İ., Yiğit, İ. N., Polat, Ü., Durgun, E., et al. (2023). Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction. Cumhuriyet Science Journal, 44(4), 716-722. https://doi.org/10.17776/csj.1342960
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