Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction

Halil İbrahim Ulusoy; İpek Nur Yiğit; Ümmügülsüm Polat; Esra Durgun; Aslıhan Gürbüzer; Songül Ulusoy

doi:10.17776/csj.1342960

EN

Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction

Abstract

An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the HPLC analysis. All parameters affecting the CPE method such as pH, buffer volume, incubation time, surfactant and electrolyte concentration, solvent for the surfactant-rich phase and its amount have been individually studied and optimized step by step. After the optimization of all parameters of the CPE process, the detection limits of the developed method for B1, B9 and B12 vitamins were calculated as 1.42 ng mL-1, 7.14 ng mL-1 and 14.28 ng mL-1 , respectively. The linear working ranges for three vitamin molecules was obtained in the range of 5.0-500.0 ng mL-1. After CPE procedure, determination of vitamin molecules was carried out by using HPLC system with diode array detector(DAD) at 244 nm for vitamin B1, 285 nm for vitamin B9, and 361 nm for vitamin B12, respectively

Keywords

References

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Details

Primary Language

English

Subjects

Bioassays , Instrumental Methods , Analytical Chemistry (Other)

Journal Section

Research Article

Authors

Halil İbrahim Ulusoy
0000-0002-8742-5145
Türkiye

İpek Nur Yiğit
0009-0009-0593-5394
Türkiye

Ümmügülsüm Polat ^*
0000-0001-8393-2396
Türkiye

Esra Durgun
0009-0009-2613-4019
Türkiye

Aslıhan Gürbüzer
0000-0002-6753-2808
Türkiye

Songül Ulusoy
0000-0003-3640-5416
Türkiye

Publication Date

December 28, 2023

Submission Date

August 16, 2023

Acceptance Date

November 30, 2023

Published in Issue

Year 2023 Volume: 44 Number: 4

DOI

https://doi.org/10.17776/csj.1342960

IZ

https://izlik.org/JA76CW53DK

Cite

RIS / Bibtex

APA

Ulusoy, H. İ., Yiğit, İ. N., Polat, Ü., Durgun, E., Gürbüzer, A., & Ulusoy, S. (2023). Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction. Cumhuriyet Science Journal, 44(4), 716-722. https://doi.org/10.17776/csj.1342960

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Öz

Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System

Sensitive Determination of Gemcitabine Molecule by Novel Magnetic Solid-Phase Extraction Coupled with HPLC-DAD