Research Article
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Effects of Some Herbs on the In-vitro Growth of Helicobacter pylori and Their Antioxidant Properties

Year 2022, Volume 43, Issue 3, 370 - 378, 30.09.2022
https://doi.org/10.17776/csj.1103225

Abstract

Helicobacter pylori is one of the important causes of chronic gastritis, peptic ulcer, stomach cancer in humans. The importance of these diseases has led to the development of effective drug treatment regimens against them. The basis of this study is the determination of the effects of some medicinal plants on the in-vitro growth of H. pylori and their antimicrobial and antioxidant potential. For this purpose, different parts of 15 plant species were extracted using solvents. Water, ethanol, chloroform, acetone extracts of plants were used and antimicrobial activities of these extracts against both H. pylori and other test microorganisms were investigated using the agar disc diffusion methods. The antioxidant properties of the extracts, which were found to be effective in terms of antimicrobial activity, were determined by the thiocyanate method. As a result, acetone extracts of plants; It was determined that it showed higher antimicrobial activity than water, ethanol, chloroform extracts. The most effective two plants against H. pylori for water, ethanol, chloroform, acetone were Capsella bursa-pastoris, Acorus calamus; Acorus calamus, Achillea millefolium; Acorus calamus, Pimpinella anisum, Acorus calamus, Achillea millefolium, respectively. Acetone extracts of Hypericum perforatum, Rosmarinus officinalis, Achillea millefolium; Acorus calamus, Pimpinella anisum plants were found to have antioxidant properties.

References

  • [1] Madigan M. T., Martingo J. M., Brock Mikroorganizmaların Biyolojisi, 11. Baskı. Ankara, (2010) 866-867.
  • [2] Widelski J., Okin´czyc P., Paluch E., Mroczek T., Szperlik J., Z˙uk M., Sroka Z., Sakipova Z., Chinou I., Skalicka-Woz´niak K., Malm A., Korona-Głowniak I., The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori, Pathogens, 11 (2022) 191.
  • [3] Mobley H. L. T., Mendz G. L., Hazell, S. L., Helicobacter pylori Physiology and Genetics, Washington, (2001).
  • [4] Guerra-Valle M., Orellana-Palma P., Petzold G., Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota, Antioxidants, 11 (2022) 109.
  • [5] Tabak M, Armon R., Neoman I., Cinnamon extracts inhibitory effect on Helicobacter pylori, J. Ethnopharmacol, 67 (1999) 269-277.
  • [6] Korona-Glowniak I., Glowniak-Lipa A., Ludwiczuk A., Baj T., Malm A., The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity,Molecules, 25 (2020) 586.
  • [7] Yan J., Peng C., Chen P., Zhang W., Jiang C., Sang S., Zhu W., Yuan Y., Yanjun Hong Y., Yao M., In-vitro anti-Helicobacter pylori activity and preliminary mechanism of action of Canarium album Raeusch. fruit extracts, J. Ethnopharmacol, 283 (2022) 114578.
  • [8] Ali S. M., Khan A. A, Ahmed I, Musaddiq M., Ahmed K. S., Polasa H., Rao L W., Habibullah C. M., Sechi L. A., Ahmed N., Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori, Ann. Clin. Microbiol. Antimicrob, 4 (2005) 20.
  • [9] Villalva M., Silvan J. M., Guerrero-Hurtado E., Gutierrez-Docio A., Navarro del Hierro J., Alarcón-Cavero T., Prodanov M., Martin D., Martinez-Rodriguez A. J., Influence of In Vitro Gastric Digestion of Olive Leaf Extracts on Their Bioactive Properties against H. pylori., Foods, 11 (2022) 1832.
  • [10] Fabry W., Okemo P., Mwatha W. E., Chhabra S. C., Ansorg R., Susceptibility of Helicobacter pylori and Candida spp. to the East African plant Terminalia spinosa, Arzneimittel-Forschung/Drug Research, 46 (1) (1996b) 539-540.
  • [11] Yeşilada E., Gürbüz İ., Shibata H., Screening of Turkish anti-ulcerrogenic folk remedies for anti -Helicobacter pylori activity, J. Ethnopharmacol, 66 (1999) 289-293.
  • [12] Davis, P. H., Flora of Turkey and the East Aegan Islands. Edinburgh, (1988).
  • [13] Baytop T (1999). Türkiye’de Bitkiler ile Tedavi,İstanbul, (1999) 480.
  • [14] Aslan A., Güllüce M., Öğütçü H., Bazı likenlerin antimikrobiyal aktiviteleri üzerine bir araştırma, Biyoteknoloji (Kükem) Dergisi, 22 (2) (1999) 19-26.
  • [15] Malekzadeh F., Ehsanifar H., Shahamat M., Levin M., Colwell R. R., Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori, Int. J. Antimicrob Ag., 18 (2001) 85-88.
  • [16] Bauer A.W., Kirby W. M. M., Sherris J. C., Turk, M., Antibiotic susceptibility testing by a standardized single disk method, American. J. Clin. Pathol., 36 (1966) 493-496.
  • [17] Ingolfsdottir K., Hjalmarsdottir M.A., Sigurdsson A., Gudjonsdottir G. A., Brynjolfsdottir A., Steingrimsson O., In vitro susceptibility of Helicobacter pylori to protolichesterinic acid from the Lichen Cetraria islandica, Antimicrob. Agents Chemother, 41(1) (1997) 215-217.
  • [18] Ali-Shtayeh S., Yaghmour M. R., Faidi Y. R., Salem K., Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area, J. Ethnopharmacol, 60 (1998) 265-271.
  • [19] Boyanova L., Neshev G., Inhibitory effect of rose oil products on Helicobacter pylori growth invitro: preminilary report, J. Med. Microbiol., 48 (1999) 705-706.
  • [20] Kadota S., Basnet P., Ishii E., Tamura T., Namba, T., Antibacterial activity of tricorabdal a from Rabdasia trichocarpa against Helicobacter pylori, Zbl Bakt., 286 (1997) 63-67.
  • [21] Srinivasan D., Nathan S,, Suresh T., Perumalsamy P.L., Antimicrobial activity of certain İndian medicinal plants used in folcloric medicine, J. Ethnopharmacol., 74 (2001) 217-220.
  • [22] Yıldırım A., Mavi A., Kara A. A., Determination of antioxidant and antimicrobial activities of Rumex cripus L. Extracts, J. Agric. Food Chem., 49 (2001) 4083-4089.
  • [23] Valsaraj R., Pushpangadan P., Smitt V. W., Adhersen A., Nyman U., Antimicrobial screening of selected medicinal plants from İndia, J. Ethnopharmacol., 58 (1997) 75-83.
  • [24] Kubo J., Lee J. R., Kubo I., Anti-Helicobacter pylori agents from the cashew apple, J. Agric. Food Chem., 47 (1999) 533-537.
  • [25] Yen G. C., Chen H. Y., Antioxidant activity of various tea extracts in relation to their antimutagenicity, J. Agric. Food Chem., 43 (1995) 27-32.
  • [26] Eloff J. N., A sensitive and Quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria, Planta Med., 64 (1998) 711-713.
  • [27] Annuk H., Hirmo S., Türi E., Mikelsaar M., Arak E., Wadström T., Effect on cell surface hydrophobicity an susceptibility of Helicobacter pylori to medicinal plant extracts, FEMS Microbiol. Lett., 172 (1999) 41-45
  • [28] Sharifi A., Azizi M., Moradi-Choghakabodi P., Aghaei S., Aziz A., In vitro anti-Helicobacter pylori activity of aqueous extract from Persian Oak testa, Chin. Herb. Med.,11(4) (2019) 394-399.
  • [29] Shen X., Zhan W., Peng C.,, Yan J., Chen P., Jiang C., Yuan Y.,, Chen D., Zhu W., Yao M.,In vitro anti‐bacterial activity and network pharmacology analysis of Sanguisorba officinalis L. against Helicobacter pylori infection,Chin Med., 16(33) (2021) 2-19.
  • [30] Ibrahim N. H., Awaad A. S., Alnafisah R. A., Alqasoumi S. I., El-Meligy R. M., Mahmoud A. Z., In–vitro activity of Desmostachya bipinnata (L.) Stapf successive extracts against Helicobacter pylori clinical isolates, Saudi Pharm J., 26 (2018) 535–540.
  • [31] Cáceres A., Fletes L., Aguilar L., Ramirez O., Figueroa L., Taracena A. M.,Samayoa B., Plants used in Guatemala for the treatment of gastrointestinal disorders. 3. confirmation of activity against enterobacteria of 16 plants, J. Ethnopharmacol., 38 (1993) 31-38.
  • [32] Takashima J., Chiba N., Yoneda K., Ohsaki A., Derrisin,a new rotenoid from Derris malaccensis Plain and Anti-Helicobacter pylori activity of its related constituents, J. Nat. Prod., 65 (2002) 611-613.
  • [33] Ohsaki A., Takashima J., Chiba, N., Kawamura M., Microanalysis of selective potent anti- Helicobacter pylori compound in a Brazilian medicinal plant, Myroxylon peruiferum and the activity of analogues., Bioorg. Med. Chem. Lett., 9 (1999) 1109-1112.
  • [34] Urzua A., Caroli M., Vasquez L., Mendoza L., Wilkens M., Tojo E., Antimicrobial study of the resinous exudate and of diterpenoids isolated from Eupatorium salvial (Asteraceae)., J. Ethnopharmacol, 62 (1998) 251-254.
  • [35] Panizzi L., Caponi C., Catalano S., Cioni P. L., Morelli I., In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius, J. Ethnopharmacol, 79 (2002) 165-168.
  • [36] Heisey R. M., Gorham, B. K., Antimicrobial effects of plant extracts on Streptococcus mutans, Candida albicans, Trichophyton rubrum and other microorganism, Lett Appl Microbiol,.14 (1992) 136-139.
  • [37] Mavi, A., Polyganum cognatum Meissn (Madımak) ve Rumex cripsus L (Evelik) bitkilerinin antioksidant aktivitelerinin mukayesesi,Yüksek Lisans, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, (2000).
  • [38] Gulcin İ., Antioxidants and antioxidant methods: an updated overview, Archiv. Toxicol., (2020).
  • [39] Mwamatopea B, Tembo D, Chikowe I, Kampirad E.,, Nyirendae C.,Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants, Scientific African, 9 (2020).
  • [40] Akhtara M. S., Rafiullah M., Shehataa W. A., Hossain A., Alib M., Comparative phytochemical, thin layer chromatographic profiling and antioxidant activity of extracts from some Indian herbal drugs, J. Bioresour. Bioprod., 7 (2022) 128–134.

Year 2022, Volume 43, Issue 3, 370 - 378, 30.09.2022
https://doi.org/10.17776/csj.1103225

Abstract

References

  • [1] Madigan M. T., Martingo J. M., Brock Mikroorganizmaların Biyolojisi, 11. Baskı. Ankara, (2010) 866-867.
  • [2] Widelski J., Okin´czyc P., Paluch E., Mroczek T., Szperlik J., Z˙uk M., Sroka Z., Sakipova Z., Chinou I., Skalicka-Woz´niak K., Malm A., Korona-Głowniak I., The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori, Pathogens, 11 (2022) 191.
  • [3] Mobley H. L. T., Mendz G. L., Hazell, S. L., Helicobacter pylori Physiology and Genetics, Washington, (2001).
  • [4] Guerra-Valle M., Orellana-Palma P., Petzold G., Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota, Antioxidants, 11 (2022) 109.
  • [5] Tabak M, Armon R., Neoman I., Cinnamon extracts inhibitory effect on Helicobacter pylori, J. Ethnopharmacol, 67 (1999) 269-277.
  • [6] Korona-Glowniak I., Glowniak-Lipa A., Ludwiczuk A., Baj T., Malm A., The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity,Molecules, 25 (2020) 586.
  • [7] Yan J., Peng C., Chen P., Zhang W., Jiang C., Sang S., Zhu W., Yuan Y., Yanjun Hong Y., Yao M., In-vitro anti-Helicobacter pylori activity and preliminary mechanism of action of Canarium album Raeusch. fruit extracts, J. Ethnopharmacol, 283 (2022) 114578.
  • [8] Ali S. M., Khan A. A, Ahmed I, Musaddiq M., Ahmed K. S., Polasa H., Rao L W., Habibullah C. M., Sechi L. A., Ahmed N., Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori, Ann. Clin. Microbiol. Antimicrob, 4 (2005) 20.
  • [9] Villalva M., Silvan J. M., Guerrero-Hurtado E., Gutierrez-Docio A., Navarro del Hierro J., Alarcón-Cavero T., Prodanov M., Martin D., Martinez-Rodriguez A. J., Influence of In Vitro Gastric Digestion of Olive Leaf Extracts on Their Bioactive Properties against H. pylori., Foods, 11 (2022) 1832.
  • [10] Fabry W., Okemo P., Mwatha W. E., Chhabra S. C., Ansorg R., Susceptibility of Helicobacter pylori and Candida spp. to the East African plant Terminalia spinosa, Arzneimittel-Forschung/Drug Research, 46 (1) (1996b) 539-540.
  • [11] Yeşilada E., Gürbüz İ., Shibata H., Screening of Turkish anti-ulcerrogenic folk remedies for anti -Helicobacter pylori activity, J. Ethnopharmacol, 66 (1999) 289-293.
  • [12] Davis, P. H., Flora of Turkey and the East Aegan Islands. Edinburgh, (1988).
  • [13] Baytop T (1999). Türkiye’de Bitkiler ile Tedavi,İstanbul, (1999) 480.
  • [14] Aslan A., Güllüce M., Öğütçü H., Bazı likenlerin antimikrobiyal aktiviteleri üzerine bir araştırma, Biyoteknoloji (Kükem) Dergisi, 22 (2) (1999) 19-26.
  • [15] Malekzadeh F., Ehsanifar H., Shahamat M., Levin M., Colwell R. R., Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori, Int. J. Antimicrob Ag., 18 (2001) 85-88.
  • [16] Bauer A.W., Kirby W. M. M., Sherris J. C., Turk, M., Antibiotic susceptibility testing by a standardized single disk method, American. J. Clin. Pathol., 36 (1966) 493-496.
  • [17] Ingolfsdottir K., Hjalmarsdottir M.A., Sigurdsson A., Gudjonsdottir G. A., Brynjolfsdottir A., Steingrimsson O., In vitro susceptibility of Helicobacter pylori to protolichesterinic acid from the Lichen Cetraria islandica, Antimicrob. Agents Chemother, 41(1) (1997) 215-217.
  • [18] Ali-Shtayeh S., Yaghmour M. R., Faidi Y. R., Salem K., Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area, J. Ethnopharmacol, 60 (1998) 265-271.
  • [19] Boyanova L., Neshev G., Inhibitory effect of rose oil products on Helicobacter pylori growth invitro: preminilary report, J. Med. Microbiol., 48 (1999) 705-706.
  • [20] Kadota S., Basnet P., Ishii E., Tamura T., Namba, T., Antibacterial activity of tricorabdal a from Rabdasia trichocarpa against Helicobacter pylori, Zbl Bakt., 286 (1997) 63-67.
  • [21] Srinivasan D., Nathan S,, Suresh T., Perumalsamy P.L., Antimicrobial activity of certain İndian medicinal plants used in folcloric medicine, J. Ethnopharmacol., 74 (2001) 217-220.
  • [22] Yıldırım A., Mavi A., Kara A. A., Determination of antioxidant and antimicrobial activities of Rumex cripus L. Extracts, J. Agric. Food Chem., 49 (2001) 4083-4089.
  • [23] Valsaraj R., Pushpangadan P., Smitt V. W., Adhersen A., Nyman U., Antimicrobial screening of selected medicinal plants from İndia, J. Ethnopharmacol., 58 (1997) 75-83.
  • [24] Kubo J., Lee J. R., Kubo I., Anti-Helicobacter pylori agents from the cashew apple, J. Agric. Food Chem., 47 (1999) 533-537.
  • [25] Yen G. C., Chen H. Y., Antioxidant activity of various tea extracts in relation to their antimutagenicity, J. Agric. Food Chem., 43 (1995) 27-32.
  • [26] Eloff J. N., A sensitive and Quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria, Planta Med., 64 (1998) 711-713.
  • [27] Annuk H., Hirmo S., Türi E., Mikelsaar M., Arak E., Wadström T., Effect on cell surface hydrophobicity an susceptibility of Helicobacter pylori to medicinal plant extracts, FEMS Microbiol. Lett., 172 (1999) 41-45
  • [28] Sharifi A., Azizi M., Moradi-Choghakabodi P., Aghaei S., Aziz A., In vitro anti-Helicobacter pylori activity of aqueous extract from Persian Oak testa, Chin. Herb. Med.,11(4) (2019) 394-399.
  • [29] Shen X., Zhan W., Peng C.,, Yan J., Chen P., Jiang C., Yuan Y.,, Chen D., Zhu W., Yao M.,In vitro anti‐bacterial activity and network pharmacology analysis of Sanguisorba officinalis L. against Helicobacter pylori infection,Chin Med., 16(33) (2021) 2-19.
  • [30] Ibrahim N. H., Awaad A. S., Alnafisah R. A., Alqasoumi S. I., El-Meligy R. M., Mahmoud A. Z., In–vitro activity of Desmostachya bipinnata (L.) Stapf successive extracts against Helicobacter pylori clinical isolates, Saudi Pharm J., 26 (2018) 535–540.
  • [31] Cáceres A., Fletes L., Aguilar L., Ramirez O., Figueroa L., Taracena A. M.,Samayoa B., Plants used in Guatemala for the treatment of gastrointestinal disorders. 3. confirmation of activity against enterobacteria of 16 plants, J. Ethnopharmacol., 38 (1993) 31-38.
  • [32] Takashima J., Chiba N., Yoneda K., Ohsaki A., Derrisin,a new rotenoid from Derris malaccensis Plain and Anti-Helicobacter pylori activity of its related constituents, J. Nat. Prod., 65 (2002) 611-613.
  • [33] Ohsaki A., Takashima J., Chiba, N., Kawamura M., Microanalysis of selective potent anti- Helicobacter pylori compound in a Brazilian medicinal plant, Myroxylon peruiferum and the activity of analogues., Bioorg. Med. Chem. Lett., 9 (1999) 1109-1112.
  • [34] Urzua A., Caroli M., Vasquez L., Mendoza L., Wilkens M., Tojo E., Antimicrobial study of the resinous exudate and of diterpenoids isolated from Eupatorium salvial (Asteraceae)., J. Ethnopharmacol, 62 (1998) 251-254.
  • [35] Panizzi L., Caponi C., Catalano S., Cioni P. L., Morelli I., In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius, J. Ethnopharmacol, 79 (2002) 165-168.
  • [36] Heisey R. M., Gorham, B. K., Antimicrobial effects of plant extracts on Streptococcus mutans, Candida albicans, Trichophyton rubrum and other microorganism, Lett Appl Microbiol,.14 (1992) 136-139.
  • [37] Mavi, A., Polyganum cognatum Meissn (Madımak) ve Rumex cripsus L (Evelik) bitkilerinin antioksidant aktivitelerinin mukayesesi,Yüksek Lisans, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, (2000).
  • [38] Gulcin İ., Antioxidants and antioxidant methods: an updated overview, Archiv. Toxicol., (2020).
  • [39] Mwamatopea B, Tembo D, Chikowe I, Kampirad E.,, Nyirendae C.,Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants, Scientific African, 9 (2020).
  • [40] Akhtara M. S., Rafiullah M., Shehataa W. A., Hossain A., Alib M., Comparative phytochemical, thin layer chromatographic profiling and antioxidant activity of extracts from some Indian herbal drugs, J. Bioresour. Bioprod., 7 (2022) 128–134.

Details

Primary Language English
Subjects Biology
Journal Section Natural Sciences
Authors

Ayşe KARA> (Primary Author)
ATATÜRK ÜNİVERSİTESİ
0000-0002-1889-3498
Türkiye


Ömer Faruk ALGUR>
ATATÜRK ÜNİVERSİTESİ
0000-0001-6447-3454
Türkiye


Ahmet MAVİ>
ATATÜRK ÜNİVERSİTESİ
0000-0003-1207-9783
Türkiye


Ali YILDIRIM>
MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ
0000-0002-0735-3728
Türkiye


Meryem ŞENGÜL KÖSEOĞLU>
ATATÜRK ÜNİVERSİTESİ
0000-0001-6050-0304
Türkiye

Publication Date September 30, 2022
Application Date April 13, 2022
Acceptance Date September 7, 2022
Published in Issue Year 2022, Volume 43, Issue 3

Cite

APA Kara, A. , Algur, Ö. F. , Mavi, A. , Yıldırım, A. & Şengül Köseoğlu, M. (2022). Effects of Some Herbs on the In-vitro Growth of Helicobacter pylori and Their Antioxidant Properties . Cumhuriyet Science Journal , 43 (3) , 370-378 . DOI: 10.17776/csj.1103225