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Green Synthesis of Gold Nanoparticles Using Aqueous Extract of Asphodelus Aestivus, Coating with Chitosan Biopolymer and Cytotoxicity Studies

Year 2022, Volume 43, Issue 3, 416 - 422, 30.09.2022
https://doi.org/10.17776/csj.1077429

Abstract

The green synthesis of gold nanoparticles (Au-NPs) was carried out by pouring the aqueous extract of East Anatolian origin Asphodelus aestivus plant onto aqueous gold metal ions and reducing them via single-step one- pot method. The absorption peak of the synthesized nanoparticles gave a maximum at 575 nm. All the X-ray diffraction peaks at 2θ = 38.25 , 44.46 , 64.64 and 77.20 that index to (111), (200), (220), and (311) planes verify the successful synthesis of Au-NPs. Mostly spherical shape particles showed a homogeneous distribution with size range 20±5 nm are measured using TEM. From the FTIR spectrum, the peaks are seems to be related to phenolic compounds, flavonoids, benzophenones, terpenoids and anthocyanins which assume that they could act as the reducing agents. The plant extraction, one-pot, single-step method used is environmentally safe without the role of synthetic materials which is highly potential in mild and green synthesis applications. The Au-NPs were coated with chitosan biopolymer in aquatic solution medium and verified by SEM. Then, cytotoxic investigations of the biosynthesized Au-NPs were carried out by HUVEC cells. Au-NPs were showed toxic effects on cell culture, even if in a small amount. However, chitosan biopolymer coating increased cell viability.

Keywords: Gold nanoparticle, Biosynthesis, Asphodelus aestivus, Biopolymer coating, Cytotoxicity

References

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  • [10] Mittal A.K., Chisti Y., Banerjee U.C., Synthesis of Metallic Nanoparticles Using Plant Extracts, Biotechnology Advances, 31/2 (2013) 346-356.
  • [11] Lee K.X., Shameli K., Miyake M., Kuwano N., Khairudin N.B.A., Mohamad S.E.B., Yew Y.P., Green synthesis of gold nanoparticles using aqueous extract of Garcinia mangostana fruit peels, Journal of Nanomaterials, (2016) Article ID 8489094.
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  • [14] Shakeel A., Annu S.I., Salprima Y.S., Biosynthesis of gold nanoparticles: A green approach, Journal of Photochemistry and Photobiology B: Biology, 161 (2016) 141-153.
  • [15] Singh P., Kim Y.-J., Zhang D., Yang D.-C., Biological Synthesis of Nanoparticles from Plants and Microorganisms, Trends in Biotechnology, 34 (7) (2016) 588-599.
  • [16] Nathanael A.J., Oh T.H. Biopolymer Coatings for Biomedical Applications, Polymers, 12(12) (2020) 3061.
  • [17] Wang Y., Dave R. N., Pfeffer R., Polymer coating/encapsulation of nanoparticles using a supercritical anti-solvent process, The Journal of Supercritical Fluids, 28(1) (2004) 85-99.
  • [18] Neuberger T., Schopf B., Hofmann H., Hofmann M., von Rechenberga B., Superparamagnetic Nanoparticles for Biomedical Applications: Possibilities and Limitations of a New Drug Delivery System, Journal of Magnetism and Magnetic Materials, 293 (2005) 483–496.
  • [19] Mossman T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65 (1983) 55-63.
  • [20] Shameli K., Ahmad M.B., Shabanzadeh P., Effect of curcuma longa tuber powder extract on size of silver nanoparticles prepared by green method. Research on Chemical Intermediates, Research on Chemical Intermediates, 40 (2014) 1313–1325.
  • [21] Cao Y., Gong Y., Liu L., The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review, Journal of Applied Toxicology, 37(12) (2017) 1359–1369.
  • [22] Mahdavi M., Namvar F., Ahmad M.B., Mohamad R., Green biosynthesis and characterization of magnetic iron oxide (Fe₃O₄) nanoparticles using seaweed (Sargassum muticum) aqueous extract, Molecules (Basel, Switzerland), 18(5) (2013) 5954–5964.
  • [23] Faried M., Shameli K., Miyake M., Hajalilou A., Synthesis of silver nanoparticles via green method using ultrasound irradiation in seaweed Kappaphycus alvarezii media, Research on Chemical Intermediates, 42 (2016) 7991–8004.
  • [24] Sharma R.K., Tahiliani S., Jain N., Priyadarshi R., Chhangani S., Purohit S.D., Joshi P., Cynodon dactylon Leaf Extract Assisted Green Synthesis of Silver Nanoparticles and Their Anti-Microbial Activity, Advanced Science, Engineering and Medicine, 5(8) (2013) 858-863.

Year 2022, Volume 43, Issue 3, 416 - 422, 30.09.2022
https://doi.org/10.17776/csj.1077429

Abstract

References

  • [1] Yilmaz E., Ates M., Sahilli Y.Ç., Synthesizing, chitosan coating and detecting the nanotoxic effect of the lead selenide (PbSe) quantum dots, Digest Journal of Nanomaterials and Biostructures, 13(4) (2018) 1173-1182.
  • [2] Rao K.J., Paria S., Aegle marmelos Leaf Extract and Plant Surfactants Mediated Green Synthesis of Au and Ag Nanoparticles by Optimizing Process Parameters Using Taguchi Method, ACS Sustainable Chemistry and Engineering, 3(3) (2015) 483–491.
  • [3] Wu Y., Ali M.R.K., Chen K., Fang N., El-Sayed M.A., Gold nanoparticles in biological optical imaging, Nano Today, 24 (2019) 120-140.
  • [4] Male D., Gromnicova R., McQuaid C., In: Al-Jamal K.T. (Eds). Gold Nanoparticles for Imaging and Drug Transport to the CNS, International Review of Neurobiology. Massachusetts: Academic Press (130) (2016) 155-198.
  • [5] Yong K.T., Swihart M.T., Ding H., Prasad P.N., Preparation of Gold Nanoparticles and their Applications in Anisotropic Nanoparticle Synthesis and Bioimaging, Plasmonics, 4(2) (2009) 79–93.
  • [6] Sanket S., Das S.K., Role of Enzymes in Synthesis of Nanoparticles. In: Thatoi H., Mohapatra S., Das S.K. (Eds). Bioprospecting of Enzymes in Industry, Healthcare and Sustainable Environment. Berlin: Springer, (2021) 139-153.
  • [7] Guilger-Casagrande M., de Lima R., Synthesis of Silver Nanoparticles Mediated by Fungi: A Review, Frontiers in bioengineering and biotechnology, 7 (2019) Article: 287.
  • [8] Khanna P., Kaur A., Goyal D., Algae-based metallic nanoparticles: Synthesis, characterization and applications, Journal of Microbiological Methods, 163 (2019) 105656.
  • [9] Kumar, S.V., Rajeshkumar, S., Plant-Based Synthesis of Nanoparticles and Their Impact. In: Tripathi D.G., Ahmad P., Sharma S., Chauhan D.K., Dubey N.K., (Eds). Nanomaterials in Plants, Algae, and Microorganisms. Massachusetts: Academic Press, 1 (2018) 33-57.
  • [10] Mittal A.K., Chisti Y., Banerjee U.C., Synthesis of Metallic Nanoparticles Using Plant Extracts, Biotechnology Advances, 31/2 (2013) 346-356.
  • [11] Lee K.X., Shameli K., Miyake M., Kuwano N., Khairudin N.B.A., Mohamad S.E.B., Yew Y.P., Green synthesis of gold nanoparticles using aqueous extract of Garcinia mangostana fruit peels, Journal of Nanomaterials, (2016) Article ID 8489094.
  • [12] Davis P.H., Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press, 8 (1984) 543-544.
  • [13] Chung IM., Park I., Seung-Hyun K., Thiruvengadam M., Rajakumar G., Plant-Mediated Synthesis of Silver Nanoparticles: Their Characteristic Properties and Therapeutic Applications, Nanoscale Research Letter, 11 (2016) Article 40.
  • [14] Shakeel A., Annu S.I., Salprima Y.S., Biosynthesis of gold nanoparticles: A green approach, Journal of Photochemistry and Photobiology B: Biology, 161 (2016) 141-153.
  • [15] Singh P., Kim Y.-J., Zhang D., Yang D.-C., Biological Synthesis of Nanoparticles from Plants and Microorganisms, Trends in Biotechnology, 34 (7) (2016) 588-599.
  • [16] Nathanael A.J., Oh T.H. Biopolymer Coatings for Biomedical Applications, Polymers, 12(12) (2020) 3061.
  • [17] Wang Y., Dave R. N., Pfeffer R., Polymer coating/encapsulation of nanoparticles using a supercritical anti-solvent process, The Journal of Supercritical Fluids, 28(1) (2004) 85-99.
  • [18] Neuberger T., Schopf B., Hofmann H., Hofmann M., von Rechenberga B., Superparamagnetic Nanoparticles for Biomedical Applications: Possibilities and Limitations of a New Drug Delivery System, Journal of Magnetism and Magnetic Materials, 293 (2005) 483–496.
  • [19] Mossman T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65 (1983) 55-63.
  • [20] Shameli K., Ahmad M.B., Shabanzadeh P., Effect of curcuma longa tuber powder extract on size of silver nanoparticles prepared by green method. Research on Chemical Intermediates, Research on Chemical Intermediates, 40 (2014) 1313–1325.
  • [21] Cao Y., Gong Y., Liu L., The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review, Journal of Applied Toxicology, 37(12) (2017) 1359–1369.
  • [22] Mahdavi M., Namvar F., Ahmad M.B., Mohamad R., Green biosynthesis and characterization of magnetic iron oxide (Fe₃O₄) nanoparticles using seaweed (Sargassum muticum) aqueous extract, Molecules (Basel, Switzerland), 18(5) (2013) 5954–5964.
  • [23] Faried M., Shameli K., Miyake M., Hajalilou A., Synthesis of silver nanoparticles via green method using ultrasound irradiation in seaweed Kappaphycus alvarezii media, Research on Chemical Intermediates, 42 (2016) 7991–8004.
  • [24] Sharma R.K., Tahiliani S., Jain N., Priyadarshi R., Chhangani S., Purohit S.D., Joshi P., Cynodon dactylon Leaf Extract Assisted Green Synthesis of Silver Nanoparticles and Their Anti-Microbial Activity, Advanced Science, Engineering and Medicine, 5(8) (2013) 858-863.

Details

Primary Language English
Subjects Chemistry, Multidisciplinary
Journal Section Natural Sciences
Authors

Ersen YILMAZ> (Primary Author)
MUNZUR UNIVERSITY
0000-0002-8567-1668
Türkiye


Mehmet ATEŞ>
MUNZUR UNIVERSITY
0000-0002-2764-6579
Türkiye


Muhammed ERBAY>
MUNZUR ÜNİVERSİTESİ
0000-0003-2335-1009
Türkiye

Supporting Institution Munzur Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (MUNIBAP)
Project Number YLMUB019-03
Publication Date September 30, 2022
Application Date February 23, 2022
Acceptance Date June 9, 2022
Published in Issue Year 2022, Volume 43, Issue 3

Cite

APA Yılmaz, E. , Ateş, M. & Erbay, M. (2022). Green Synthesis of Gold Nanoparticles Using Aqueous Extract of Asphodelus Aestivus, Coating with Chitosan Biopolymer and Cytotoxicity Studies . Cumhuriyet Science Journal , 43 (3) , 416-422 . DOI: 10.17776/csj.1077429