In vitro Evaluation of Antigenotoxic Effects of Phloridzin
Year 2022,
Volume: 43 Issue: 3, 358 - 364, 30.09.2022
Mehmet Sarimahmut
,
Sindi Vekshari
Merve Demirbag Karaali
Serap Celikler
Abstract
Phytochemicals have a vast number of properties contributing to human health by acting on numerous different mechanisms. Phloridzin, a phytochemical mainly found in Malus species, possesses diverse biological activities including anti-diabetic and antioxidative activities. Here, our aim is to explore antigenotoxic potential and proliferative effects of phloridzin on human lymphocytes in vitro by employing chromosome aberration, micronucleus and comet assays. Mitomycin C, both an anticancer and genotoxic agent, was utilized to induce genotoxicity. Phloridzin significantly suppressed the genotoxic effects of mitomycin C at 125-500 µg/mL concentrations in all assays used (p < 0.05). We also revealed that phloridzin and mitomycin C combination had a significantly negative effect on mitotic index (p < 0.05), whereas in general, gender differences did not play a role in manifestation of neither antigenotoxic nor antiproliferative activities of the combination.These results suggest that phloridzin is an antigenotoxic compound and its consumption may interfere with the activity of anticancer drugs that exert their effects based on genotoxic mechanisms.
Thanks
The authors would like to thank Ahmet Sari Mahmout for English editing and proofreading of the manuscript.
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Year 2022,
Volume: 43 Issue: 3, 358 - 364, 30.09.2022
Mehmet Sarimahmut
,
Sindi Vekshari
Merve Demirbag Karaali
Serap Celikler
References
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- [2] Chikara S., Nagaprashantha L.D., Singhal J., Horne D., Awasthi S., Singhal S.S., Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment, Cancer Lett., 413 (2018) 122–134.
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- [16] Kaya F.F., Topaktaş M., Genotoxic effects of potassium bromate on human peripheral lymphocytes in vitro, Mutat. Res. - Genet. Toxicol. Environ. Mutagen., 626 (1–2) (2007) 48–52.
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- [18] Fenech M., Morley A.A., Measurement of micronuclei in lymphocytes, Mutat. Res. Genet. Toxicol. Environ. Mutagen., 147(1–2) (1985) 29–36.
- [19] Singh N.P., McCoy M.T., Tice R.R., Schneider E.L., A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 175 (1988) 184–191.
- [20] Anderson D., Yu T.W., Phillips B.J., Schmezer P., The effect of various antioxidants and other modifying agents on oxygen–radical–generated DNA damage in human lymphocytes in the COMET assay, Mutat. Res., 307 (1994) 261–271.
- [21] Jamshidi–Kia F., Lorigooini Z., Amini–Khoei H., Medicinal plants: Past history and future perspective, J. HerbMed Pharmacol., 7 (1) (2018) 1–7.
- [22] Hyson D.A., A comprehensive review of apples and apple components and their relationship to human health, Adv. Nutr., 2 (5) (2011) 408–420.
- [23] Handan B.A., De Moura C.F.G., Cardoso C.M., Santamarina A.B., Pisani L.P., Ribeiro D.A., Protective Effect of Grape and Apple Juices against Cadmium Intoxication in the Kidney of Rats, Drug Res., 70 (11) (2020) 503–511.
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- [25] Cao J., Jiang L.P., Liu Y., Yang G., Yao X.F., Zhong L.F., Curcumin–induced genotoxicity and antigenotoxicity in HepG2 cells, Toxicon, 49 (8) (2007) 1219–1222.
- [26] Erdem M.G., Cinkilic N., Vatan O., Yilmaz D., Bagdas D., Bilaloglu R., Genotoxic and anti–genotoxic effects of vanillic acid against mitomycin C–induced genomic damage in human lymphocytes in vitro, Asian Pac. J. Cancer Prev., 13 (10) (2012) 4993–4998.
- [27] Błasiak J., Trzeciak A., Dziki A., Ulańska J., Pander B., Synergistic Effect of Vitamin C on DNA Damage Induced by Cadmium, Gen. Physiol. Biophys., 19 (4) (2000) 373–379.
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- [30] Verweij J., Pinedo H.M., Mitomycin C: mechanism of action, usefulness and limitations, Anticancer Drugs, 1 (1) (1990) 5–13.
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- [33] Kirsch–Volders M., Elhajouji A., Cundari E., Van Hummelen P., The in vitro micronucleus test: a multi–endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non–disjunction, Mutat. Res. Genet. Toxicol. Environ. Mutagen., 392(1–2) (1997) 19–30.
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- [35] Sun L., Sun J., Thavaraj P., Yang X., Guo Y., Effects of thinned young apple polyphenols on the quality of grass carp (Ctenopharyngodon idellus) surimi during cold storage, Food Chem., 224 (2017) 372–381.
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- [38] Lee J., Jung E., Kim Y.S., Park D., Toyama K., Date A., Lee J., Phloridzin isolated from Acanthopanax senticosus promotes proliferation of α6 integrin (CD 49f) and β1 integrin (CD29) enriched for a primary keratinocyte population through the ERK–mediated mTOR pathway, Arch. Dermatol. Res., 305 (8) (2013) 747–754.
- [39] Aliyu M., Odunola O.A., Farooq A.D., Mesaik A.M., Choudhary M.I., Azhar M., Asif M.M., Erukainure O.L., Antioxidant, mitogenic and immunomodulatory potentials of acacia honey, Nutr. Ther. Metab., 32 (2) (2014) 68–78.
- [40] Avuloglu–Yilmaz E., Yuzbasioglu D., Unal F., In vitro genotoxicity assessment of monopotassium glutamate and magnesium diglutamate, Toxicol. In Vitro, 65 (2020) 104780.