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DNA Interaction of Methylene Green and Fast Green with dsDNA and ssDNA and G-quadruplex DNA

Year 2026, Volume: 47 Issue: 1, 96 - 102, 27.02.2026

Didem Duman , Efkan Bağda , Esra Bağda

https://doi.org/10.17776/csj.1830319
https://izlik.org/JA83FL22HG

Abstract

DNA plays an active role in cellular processes not only through the transport of genetic information through its base sequence but also through the secondary structures revealed by folding. In recent years, investigating the interaction of DNA-targeting ligands has emerged as an important area of research, especially considering the importance of these structures in processes such as cancer. The current study investigated the interaction of MG and FG ligands with DNA. The main motivation for the study was to investigate whether these structures, known to interact with DNA, have a specific affinity for ctDNA, ssDNA, and G-quadruplex DNA. The interaction of FG with DNA species did not cause any significant spectral changes, thus concluding that the affinity was very low. Competitive dialysis experiments confirmed the affinity results. Significant spectral changes were observed in the MG-DNA interaction. The interaction of MG with DNA species caused a hypochromic effect of up to 52%, while a bathochromic shift of up to 17 nm was observed. Kb values ​​calculated from the data were also found to be higher than 106 M-1. The interaction stoichiometry with G-quadruplex DNAs was determined to be 1:2, indicating an end-stacking mechanism. Competitive dialysis experiments also confirmed that MG exhibited high affinity for DNA species.

Keywords

Fast Green G-quadruplex DNA Methylene Green

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References

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There are 29 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Analytical Chemistry
Journal Section Research Article
Authors

Didem Duman 0000-0002-0900-0336

Efkan Bağda 0000-0002-3925-3430

Esra Bağda 0000-0003-1900-4944

Project Number -
Submission Date November 25, 2025
Acceptance Date January 5, 2026
Publication Date February 27, 2026
DOI https://doi.org/10.17776/csj.1830319
IZ https://izlik.org/JA83FL22HG
Published in Issue Year 2026 Volume: 47 Issue: 1

Cite

APA Duman, D., Bağda, E., & Bağda, E. (2026). DNA Interaction of Methylene Green and Fast Green with dsDNA and ssDNA and G-quadruplex DNA. Cumhuriyet Science Journal, 47(1), 96-102. https://doi.org/10.17776/csj.1830319

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