Research Article
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Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro

Year 2026, Volume: 47 Issue: 1, 1 - 8, 27.02.2026

Gamze Tüzün , Ceylan Hepokur , Armagan Akkuş , Mohammad Omar Faruque , Sema Mısır

https://doi.org/10.17776/csj.1778624
https://izlik.org/JA29ST36KH

Abstract

Origanum sipyleum L.   is commonly used for the treatment of cough, pain, cancer, and infectious diseases. In this study, the antioxidant and antiproliferative properties of this endemic species Origanum sipyleum L. (purple coral) were investigated. Quantitative analysis of the components of Origanum sipyleum L. was performed by HPLC. The antioxidant effects of Origanum sipyleum L.  ethanol extract was examined. Among the antioxidant effects, Total Polyphenolic Content Determination, Total Flavonoid Content Determination, Plasma Ferric Reducing Ability Test and Radical Scavenging Activity (DPPH) Determination were examined. The cytotoxic effect was determined using the XTT assay against MCF-7 (human breast adenocarcinoma cell line), MDA-MB-231 (human breast cancer cell line), and L929 (mouse  fibroblast cell line) cells. Calculated IC50 values of Origanium Sipyleum L. were 205±6.86 against MCF-7 cells for 24 h, 147±4.23 for 48 h, 197±4.15 for 24 h and 96± 3.46 against MDA-MB-231 cells, and 415±1.67 for 24 h 221±2.13 for 48 h, against L929, respectively. Antiproliferative properties of Origanium sipyleum L. was assessed by flow cytometry. MDA-MB-231 cell lines were used to determine cell proliferation and BrdU assay was analyzed spectrophotometrically. Results postulated that Origanum sipyleum L. had a high antioxidant and antiproliferative effects.

Keywords

Antioxidant effect Antiproliferative effects BrDU assay Origanum sipyleum L.

Ethical Statement

No

Supporting Institution

Sivas Cumhuriyet University

Project Number

CUBAP project no. ECZ-059

Thanks

The Authors thanks to Dr. Mehmet ÇİÇEK for collected plant.

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There are 47 citations in total.

Details

Primary Language English
Subjects Cancer Biology
Journal Section Research Article
Authors

Gamze Tüzün 0000-0002-5876-2046

Ceylan Hepokur 0000-0001-6397-1291

Armagan Akkuş 0000-0002-8374-7892

Mohammad Omar Faruque 0000-0002-4684-5004

Sema Mısır 0000-0002-5253-0420

Project Number CUBAP project no. ECZ-059
Submission Date September 5, 2025
Acceptance Date December 30, 2025
Publication Date February 27, 2026
DOI https://doi.org/10.17776/csj.1778624
IZ https://izlik.org/JA29ST36KH
Published in Issue Year 2026 Volume: 47 Issue: 1

Cite

APA Tüzün, G., Hepokur, C., Akkuş, A., Faruque, M. O., & Mısır, S. (2026). Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. Cumhuriyet Science Journal, 47(1), 1-8. https://doi.org/10.17776/csj.1778624

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