Synthesis, BuChE and AChE Enzyme Inhibition and Molecular Docking Studies of Hydrazide-Hydrazone Schiff Base Compound Containing Propargyl/Pyridine Moiety

Özge Güngör

doi:10.17776/csj.1773859

Synthesis, BuChE and AChE Enzyme Inhibition and Molecular Docking Studies of Hydrazide-Hydrazone Schiff Base Compound Containing Propargyl/Pyridine Moiety

Abstract

The focus of this study was the preparation and structural characterisation of hydrazine hydrazone Schiff base compounds containing a propargyl group in a new structure, using common spectroscopic and analytical methods. Since Hydrazide-hydrazone compounds are known to possess high biological activity, it was thought that compound 2a synthesized by us could be an important inhibitor for the treatment of Alzheimer's disease. A screening was performed on the Hydrazide-hydrazone Schiff base compound (2a) to determine its potential as an inhibitor of cholinesterase. The enzyme inhibition values of compound 2a were determined as IC₅₀= 42.72±0.01 and IC₅₀= 39.19±0.04 µM for AChE and BuChE, respectively. Compared to the standard compound Galantamine (IC₅₀= 32.45±0.06 and IC₅₀= 40.25±0.03 µM), it exhibited low activity for AChE. It exhibited a higher activity for BuChE. When compared to the standard drug galantamine, the compound showed only moderate inhibitory activity. Ki values of 1.25 and 2.32 μM were determined as the substance exhibited milder activity against AChE and BuChE, respectively. Molecular docking studies also revealed that these hydrophilic and hydrophobic interactions are instrumental in conferring the compound's strong binding affinity. They also revealed that these interactions are instrumental in the compound's potent inhibitory activity. These results indicate that compound 2a exhibits activity against both enzymes.

Keywords

References

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Details

Primary Language

English

Subjects

Enzymes , Organic Chemical Synthesis

Journal Section

Research Article

Authors

Özge Güngör ^*
0000-0003-3529-2704
Türkiye

Publication Date

February 27, 2026

Submission Date

August 29, 2025

Acceptance Date

January 16, 2026

Published in Issue

Year 2026 Volume: 47 Number: 1

DOI

https://doi.org/10.17776/csj.1773859

IZ

https://izlik.org/JA25SS75HD

Cite

RIS / Bibtex

APA

Güngör, Ö. (2026). Synthesis, BuChE and AChE Enzyme Inhibition and Molecular Docking Studies of Hydrazide-Hydrazone Schiff Base Compound Containing Propargyl/Pyridine Moiety. Cumhuriyet Science Journal, 47(1), 103-109. https://doi.org/10.17776/csj.1773859