Investigation of Prokaryotic Diversity in Giresun Blue Lake and Examination of Certain Industrially Significant Enzyme Activities in Cultivable Isolates

Dönüş Gençer; Aydın Yeşilyurt; Esma Ceylan; Sabriye Çanakçı; Remziye Nalcacioglu; Hatice Katı

doi:10.17776/csj.1744308

Investigation of Prokaryotic Diversity in Giresun Blue Lake and Examination of Certain Industrially Significant Enzyme Activities in Cultivable Isolates

Abstract

Blue Lake, a newly discovered soda lake in Giresun Province, Türkiye, is characterized by alkaline water and moderate salinity, creating a unique and unexplored habitat for microorganisms. This study aimed to investigate the prokaryotic diversity of Blue Lake using both culture-dependent and culture-independent approaches and to evaluate the industrial enzyme-producing potential of the cultivable isolates. Physicochemical properties of water and sediment samples were analyzed, followed by 16S rRNA gene-based metagenomic sequencing and conventional cultivation techniques. Metagenomic analyses revealed a highly diverse prokaryotic community comprising 52 phyla and 311 species, with Proteobacteria being the dominant phylum (65.79 ± 4.94%) and Gammaproteobacteria being the most abundant class (46.58 ± 5.18%). Acinetobacter was identified as the predominant genus, while Sphingobacterium multivorum was the most prevalent species. A total of 87 bacterial isolates were obtained using culture-dependent methods, several of which exhibited significant enzymatic activities, including protease, lipase, amylase, cellulase, chitinase, and pectinase production. The presence of enzyme-producing alkaliphilic and halotolerant bacteria highlights the biotechnological and environmental potential of Blue Lake. This study provides the first integrated metagenomic and cultivation-based characterization of the prokaryotic ecosystem of Blue Lake, contributing valuable insights into soda lake microbiomes and their industrial relevance.

Keywords

Supporting Institution

Karadeniz Technical University

Project Number

Project No: FBA-2023-10956

Thanks

This study was financially supported by Karadeniz Technical University (Project No: FBA-2023-10956).

References

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Details

Primary Language

English

Subjects

Bioinformatics and Computational Biology (Other) , Microbial Ecology

Journal Section

Research Article

Authors

Dönüş Gençer
0000-0002-5179-3763
Türkiye

Aydın Yeşilyurt
0000-0003-4034-4206
Türkiye

Esma Ceylan
0000-0002-5559-8869
Türkiye

Sabriye Çanakçı
0000-0003-0132-7198
Türkiye

Remziye Nalcacioglu
0000-0003-0527-9541
Türkiye

Hatice Katı ^*
0000-0002-2053-3168
Türkiye

Publication Date

February 27, 2026

Submission Date

July 16, 2025

Acceptance Date

January 27, 2026

Published in Issue

Year 2026 Volume: 47 Number: 1

DOI

https://doi.org/10.17776/csj.1744308

IZ

https://izlik.org/JA27EM67ZK

Cite

RIS / Bibtex

APA

Gençer, D., Yeşilyurt, A., Ceylan, E., Çanakçı, S., Nalcacioglu, R., & Katı, H. (2026). Investigation of Prokaryotic Diversity in Giresun Blue Lake and Examination of Certain Industrially Significant Enzyme Activities in Cultivable Isolates. Cumhuriyet Science Journal, 47(1), 34-43. https://doi.org/10.17776/csj.1744308