Research Article
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Investigation of Prokaryotic Diversity in Giresun Blue Lake and Examination of Certain Industrially Significant Enzyme Activities in Cultivable Isolates

Year 2026, Volume: 47 Issue: 1, 34 - 43, 27.02.2026

Dönüş Gençer , Aydın Yeşilyurt , Esma Ceylan , Sabriye Çanakçı , Remziye Nalcacioglu , Hatice Katı

https://doi.org/10.17776/csj.1744308
https://izlik.org/JA27EM67ZK

Abstract

Blue Lake, a newly discovered soda lake in Giresun Province, Türkiye, is characterized by alkaline water and moderate salinity, creating a unique and unexplored habitat for microorganisms. This study aimed to investigate the prokaryotic diversity of Blue Lake using both culture-dependent and culture-independent approaches and to evaluate the industrial enzyme-producing potential of the cultivable isolates. Physicochemical properties of water and sediment samples were analyzed, followed by 16S rRNA gene-based metagenomic sequencing and conventional cultivation techniques. Metagenomic analyses revealed a highly diverse prokaryotic community comprising 52 phyla and 311 species, with Proteobacteria being the dominant phylum (65.79 ± 4.94%) and Gammaproteobacteria being the most abundant class (46.58 ± 5.18%). Acinetobacter was identified as the predominant genus, while Sphingobacterium multivorum was the most prevalent species. A total of 87 bacterial isolates were obtained using culture-dependent methods, several of which exhibited significant enzymatic activities, including protease, lipase, amylase, cellulase, chitinase, and pectinase production. The presence of enzyme-producing alkaliphilic and halotolerant bacteria highlights the biotechnological and environmental potential of Blue Lake. This study provides the first integrated metagenomic and cultivation-based characterization of the prokaryotic ecosystem of Blue Lake, contributing valuable insights into soda lake microbiomes and their industrial relevance.

Keywords

Acinetobacter Blue Lake Enzymatic activity Gammaproteobacteria Metagenomic

Supporting Institution

Karadeniz Technical University

Project Number

Project No: FBA-2023-10956

Thanks

This study was financially supported by Karadeniz Technical University (Project No: FBA-2023-10956).

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There are 37 citations in total.

Details

Primary Language English
Subjects Bioinformatics and Computational Biology (Other), Microbial Ecology
Journal Section Research Article
Authors

Dönüş Gençer 0000-0002-5179-3763

Aydın Yeşilyurt 0000-0003-4034-4206

Esma Ceylan 0000-0002-5559-8869

Sabriye Çanakçı 0000-0003-0132-7198

Remziye Nalcacioglu 0000-0003-0527-9541

Hatice Katı 0000-0002-2053-3168

Project Number Project No: FBA-2023-10956
Submission Date July 16, 2025
Acceptance Date January 27, 2026
Publication Date February 27, 2026
DOI https://doi.org/10.17776/csj.1744308
IZ https://izlik.org/JA27EM67ZK
Published in Issue Year 2026 Volume: 47 Issue: 1

Cite

APA Gençer, D., Yeşilyurt, A., Ceylan, E., Çanakçı, S., Nalcacioglu, R., & Katı, H. (2026). Investigation of Prokaryotic Diversity in Giresun Blue Lake and Examination of Certain Industrially Significant Enzyme Activities in Cultivable Isolates. Cumhuriyet Science Journal, 47(1), 34-43. https://doi.org/10.17776/csj.1744308

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