Network Pharmacology-Based Approach to Unveil the Therapeutic Mechanism of Viburnum opulus L. on Glomerulonephritis

Şeyda Kaya; Sevgi Durna Daştan

doi:10.17776/csj.1669595

EN

Network Pharmacology-Based Approach to Unveil the Therapeutic Mechanism of Viburnum opulus L. on Glomerulonephritis

Abstract

This study aimed to investigate the potential targets and pathways of the bioactive compounds of Viburnum opulus L. – a plant recognized in ethnopharmacology for its therapeutic applications in renal disorders - on glomerulonephritis (GN) disease. The phytochemical profile of V. opulus was obtained from review articles in the literature on plant contents. GN-associated target genes were retrieved from the GeneCards database, and interaction mapping was conducted using Cytoscape 3.10.0. The intersection of compound - target - disease was imported into the STRING database to create a PPI network. GO and KEGG enrichment analyses were applied to define the biological functions of the targets. A molecular docking study simulated the binding capabilities of key targets and active ingredients. A total of 9 bioactive constituents were identified from V. opulus, with rutin and quercetin demonstrating the highest binding affinities. The resulting compound–target interaction network consisted of 125 nodes and 358 edges. Central hub proteins within the PPI network included TP53, SRC, and EGFR indicating their potential role in the mechanism of action. GO and KEGG analyses suggested that the treatment of GN by V. opulus mainly involves the generation of cellular response to chemical and oxidative stress, protein tyrosine kinase activity, transcription regulatory complex, and other biological processes. The results of KEGG enrichment analysis indicate that V. opulus mainly involves some pathways, such as chemical carcinogenesis-receptor activation, EGFR tyrosine kinase inhibitor resistance in the treatment of GN. Molecular docking data presented that rutin and quercetin have the highest affinity score with TP53, SRC, and EGFR proteins. Overall, this study reveals the active compounds and potential molecular pathways of V. opulus in the treatment of GN and presents a source for further basic studies.

Keywords

Thanks

I am grateful to thank Sivas Cumhuriyet University for their support and permission to access the Maestro Schrödinger program.

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Details

Primary Language

English

Subjects

Biological Network Analysis , Molecular Docking

Journal Section

Research Article

Authors

Şeyda Kaya ^*
0000-0001-8489-8687
Türkiye

Sevgi Durna Daştan
0009-0003-8565-6841
Türkiye

Publication Date

June 30, 2025

Submission Date

April 4, 2025

Acceptance Date

May 28, 2025

Published in Issue

Year 2025 Volume: 46 Number: 2

DOI

https://doi.org/10.17776/csj.1669595

IZ

https://izlik.org/JA87EL96YC

Cite

RIS / Bibtex

APA

Kaya, Ş., & Durna Daştan, S. (2025). Network Pharmacology-Based Approach to Unveil the Therapeutic Mechanism of Viburnum opulus L. on Glomerulonephritis. Cumhuriyet Science Journal, 46(2), 348-359. https://doi.org/10.17776/csj.1669595

Öz

Network Pharmacology-Based Approach to Unveil the Therapeutic Mechanism of Viburnum opulus L. on Glomerulonephritis

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite