Morphological, Transcriptional, and Epigenetics Alterations due to L-arginine and L-methionine Treatment in Fusarium culmorum

Fatma Berra Yücesan; Özlem Sefer; Emre Yörük

doi:10.17776/csj.1638941

EN

Morphological, Transcriptional, and Epigenetics Alterations due to L-arginine and L-methionine Treatment in Fusarium culmorum

Abstract

Fusarium culmorum is a worldwide phytopathogenic fungus of small-grain cereals. Struggling strategies such as fungicide treatment and biocontrol agent usage are not long-term solutions due to the potential adverse effects on ecological environment and resistance development in fungal pathogens. In this study, potential suppressive effects of amino acid supplementation on F. culmorum were investigated. Potato dextrose agar (PDA) medium amended with 1 mg mL-1 and 2 mg mL-1 concentrations of L-arginine and L-methionine were used as experimental sets. PDA with no supplement and PDA amended with nicotinamide of 1 mg mL-1 and 2 mg mL-1 concentrations were used as negative and positive control sets, respectively. While L-arginine treatment led to significant increase in linear growth rate (LGR) with p<0.01, L-methionine decreased LGR values (p<0.001). Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) essays yielded very similar alterations in terms of genomic template stability within the experiment groups of L-arginine and L-methionine treated sets. UPGMA-dendrogram (unweighted pair group method with arithmetic mean) revealed co-clustering of L-methionine and nicotinamide treated sets. Methylation-specific PCR (MSP) analysis showed that there was Type-II and Type-III methylation present in 2 mg mL-1 L-methionine treated sets. Gene expression analysis showed that L-methionine and L-arginine treatment led to contrast alteration in expressions of tri6 and FcStuA genes with significant differences (p<0.05-p<0.0001). Our results showed that L-methionine treatment could suppress potential aggressiveness of F. culmorum at phenotypic, epigenetics, and transcriptional levels

Keywords

Supporting Institution

This study was supported by Scientific and Technological Research Council of Türkiye (TUBITAK) under Grant Number 1919B012310447 (2209-A). The authors thank TUBITAK for their support.

Project Number

1919B012310447 (2209-A)

Thanks

This study was supported by Scientific and Technological Research Council of Türkiye (TUBITAK) under Grant Number 1919B012310447 (2209-A). The authors thank TUBITAK for their support.

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Details

Primary Language

English

Subjects

Gene Expression

Journal Section

Research Article

Authors

Fatma Berra Yücesan
0009-0007-3289-8731
Türkiye

Özlem Sefer
0000-0002-2711-5938
Türkiye

Emre Yörük ^*
0000-0003-2770-0157
Türkiye

Publication Date

June 30, 2025

Submission Date

February 13, 2025

Acceptance Date

May 11, 2025

Published in Issue

Year 2025 Volume: 46 Number: 2

DOI

https://doi.org/10.17776/csj.1638941

IZ

https://izlik.org/JA32US56PW

Cite

RIS / Bibtex

APA

Yücesan, F. B., Sefer, Ö., & Yörük, E. (2025). Morphological, Transcriptional, and Epigenetics Alterations due to L-arginine and L-methionine Treatment in Fusarium culmorum. Cumhuriyet Science Journal, 46(2), 257-263. https://doi.org/10.17776/csj.1638941