Determination of Total Phenolic Amounts of Chloroform, Acetone and Methanol Extracts of Sargassum sp., Ulva sp. and Schizochytrium sp.

Mehmet Naz

doi:10.17776/csj.1629561

EN TR

Determination of Total Phenolic Amounts of Chloroform, Acetone and Methanol Extracts of Sargassum sp., Ulva sp. and Schizochytrium sp.

Abstract

The present study was aimed to determine the total phenolic amounts of the bioactive contents resulting from the extraction of Sargassum sp., Ulva sp. and Schizochytrium sp. with different solvents such as chloroform, acetone and methanol. Total phenolic content was determined using the Folin-Ciocalteu method. Samples (12.5%) were extracted with chloroform, acetone, and methanol, then filtered and the solvents were evaporated. Absorbance was measured at 760 nm. Total phenolic content was expressed as gallic acid equivalents (GAE). The total phenolic contents obtained from chloroform, acetone and methanol extracts of Sargassum sp., Ulva sp. and Schizochytrium sp. were determined as 136.40±1.93 mg GAE/g extract -3.61±0.18 mg GAE/g extract -7.83±0.03 mg GAE/g extract, 5.25±0.07 mg GAE/g extract -4.27±0.1 mg GAE/g extract -14.5±0.06 mg GAE/g extract and 15.7±0.27 mg GAE/g extract -2.6±0.04 mg GAE/g extract -14.84±0.11 mg GAE/g extract, respectively. Differences between total phenolic values were found to be statistically significant (p<0.05). The extraction yields obtained from chloroform, acetone and methanol extracts of Sargassum sp., Ulva sp. and Schizochytrium sp. were determined as 2.2±0.06%-4.35±0.07%-6.68±0.14%, 2.96±0.02%-4.33±0.09%-9.74±0.16% and 14.96±0.24%-10.03±0.004%-19.03±0.3%, respectively (p<0.05). Biochemical parameters such as dry matter, protein, ash and lipid of Sargassum sp., Ulva sp. and Schizochytrium sp. were found to be 94.05±0.01% -20.92±0.35% -26.55±1.77% -1.36±0.01%, 94.1±0.005%-17.04±0.04%-28.11±0.06% -2.66±0.05% and 95.1±0.05%- 23.01±0.07%- 9.59±0.787%- 25.5±0.21%. The soluble protein concentrations of Sargassum sp., Ulva sp. and Schizochytrium sp. in distilled water were determined as 66.45±0.5mg/g, 83.38±0.88 mg/g and 115.95±0.89 mg/g, respectively. In conclusion, chloroform and methanol extracts of Schizochytrium sp. had good extraction yield and phenolic content. On the other hand, the chloroform extract of Sargassum sp. had the highest phenolic content, while the extraction yield was at the lowest level. Acetone extracts of the 3 species tested exhibited low phenolic activity.

Keywords

Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin Kloroform, Aseton ve Metanol Ekstraktlarının Toplam Fenolik Miktarlarının Belirlenmesiı

Öz

Mevcut çalışmada, Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin kloroform, aseton ve metanol gibi farklı solventlerle ekstrakte edilmesi sonucu ortaya çıkan biyoaktif içeriğin toplam fenolik miktarlarının belirlenmesi amaçlanmıştır. Aynı zamanda Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin biyokimyasal kompozisyonları, farklı solventlerden alınan ekstraksiyon ürünü ve çözülebilir protein konsantrasyonlarıda araştırılmıştır. Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin kloroform, aseton ve metanol ekstraktlarından elde edilen toplam fenolik içerikleri sırasıyla 136,40±1,93 mg GA/g ekstrakt -3,61±0,18 mg GA/g ekstrakt -7,83±0,03 mg GA/g ekstrakt, 5,25±0,07 mg GA/g ekstrakt -4,27±0,1 mg GA/g ekstrakt -14,5±0,06 mg GA/g ekstrakt 15,7±0,27 mg GA/g ekstrakt -2,6±0,04 mg GA/g ekstrakt -14,84±0,11 mg GA/g ekstrakt olarak belirlenmiş olup, değerler arasındaki farklılıklar istatistiksel olarak önemli bulunmuştur (p<0,05). Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin kloroform, aseton ve metanol ekstraktlarından elde edilen ekstraksiyon ürün miktarları sırasıyla %2,2±0,06-%4,35±0,07-%6,68±0,14, %2,96±0,02-%4,33±0,09-%9,74±0,16 ve %14,96±0,24-%10,03±0,004-%19,03±0,3 olarak belirlenmiştir (p<0,05). Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin kuru madde, protein, kül ve lipit gibi biyokimyasal parametreleri sırasıyla %94,05±0,01 -%20,92±0,35 -%26,55±1,77-%1,36±0,01, %94,1±0,005-%17,04±0,04-%28,11±0,06 -%2,66±0,05 ve %95,1±0,05- %23,01±0,07- %9,59±0,787- %25,5±0,21 olarak bulunmuştur (p<0,05). Sargassum sp., Ulva sp. ve Schizochytrium sp.’nin distile sudaki çözülebilir protein konsantrasyonu sırasıyla 66,45±0,5mg/g, 83,38±0,88 mg/g ve 115,95±0,89 mg/g olarak tespit edilmiştir (p<0,05). Schizochytrium sp.’nin kloroform ve metanol ekstrakları iyi ekstraksiyon ürünü ve fenolik içeriğe sahipti. Buna karşılık Sargassum sp.’nin kloroform ekstraktı en yüksek fenolik içeriğe sahipken ekstraksiyon ürünü en düşük seviyedeydi. Test edilen 3 türün aseton ekstrakları düşük fenolik aktivite sergiledi.

Anahtar Kelimeler

Ethical Statement

Since no human or animal subjects were used in this study, ethical approval was not required.

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Mehmet Naz ^*
0000-0002-5129-8498
Türkiye

Publication Date

June 30, 2025

Submission Date

January 30, 2025

Acceptance Date

May 27, 2025

Published in Issue

Year 2025 Volume: 46 Number: 2

DOI

https://doi.org/10.17776/csj.1629561

IZ

https://izlik.org/JA69HU42HK

Cite

RIS / Bibtex

APA

Naz, M. (2025). Determination of Total Phenolic Amounts of Chloroform, Acetone and Methanol Extracts of Sargassum sp., Ulva sp. and Schizochytrium sp. Cumhuriyet Science Journal, 46(2), 292-297. https://doi.org/10.17776/csj.1629561