Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System

Halil İbrahim Ulusoy; Seren Kilit; Ümmügülsüm Polat; Esra Durgun; Aslıhan Gürbüzer; Elif Tümay Özer; Bilgen Osman

doi:10.17776/csj.1572205

EN

Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System

Abstract

An analytical approach has been developed for the sensitive determination of Phenobarbital, which is used as an antiepileptic drug molecule. The analysis of this active molecule, which has a very limited study even for its direct determination in the literature review, is mostly done with highly complex device systems such as LC-MS. In analysis with conventional HPLC systems, the limit of detection cannot be in most cases lower than 1 µg mL-1 With this study, a separation and preconcentration method based on solid phase extraction (SPE) was developed for trace phenobarbital molecules, so that even very low concentrations could be monitored. In the proposed method, the target molecules were enriched with column type SPE method, and then their analysis were carried out by with the HPLC-DAD system. As an SPE sorbent, a polymeric material, poly(ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophan methyl ester) [poly(EGDMA-MATrp)], was used in extraction experiments. Experimental variables such as pH of medium, type and amount of desorption solvent, electrolyte effect have been studied and optimized step by step. The linear working range under the optimized conditions were determined in the range of 10.00-400.00 ng mL-1 with the limit of detection as 3.57 ng mL-1. Quantitative results were obtained in recovery experiments with the help of model solutions including phenobarbital molecule

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry , Separation Science

Journal Section

Research Article

Authors

Halil İbrahim Ulusoy ^*
0000-0002-8742-5145
Türkiye

Seren Kilit
0009-0003-4453-4215
Türkiye

Ümmügülsüm Polat
0000-0001-8393-2396
Türkiye

Esra Durgun
0009-0009-2613-4019
Türkiye

Aslıhan Gürbüzer
0000-0002-6753-2808
Türkiye

Elif Tümay Özer
0000-0002-5225-0146
Türkiye

Bilgen Osman
0000-0001-8406-149X
Türkiye

Publication Date

December 30, 2024

Submission Date

October 23, 2024

Acceptance Date

December 19, 2024

Published in Issue

Year 2024 Volume: 45 Number: 4

DOI

https://doi.org/10.17776/csj.1572205

IZ

https://izlik.org/JA94LP86FT

Cite

RIS / Bibtex

APA

Ulusoy, H. İ., Kilit, S., Polat, Ü., Durgun, E., Gürbüzer, A., Tümay Özer, E., & Osman, B. (2024). Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System. Cumhuriyet Science Journal, 45(4), 763-768. https://doi.org/10.17776/csj.1572205

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