Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line

Zekeriya Keskin; Fatih Yulak; Hatice Terzi; Merve İnanır

doi:10.17776/csj.1344535

EN

Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line

Abstract

There are many challenges in the treatment of Burkitt lymphoma, especially in immunocompromised individuals, elderly patients, and patients with relapsed or refractory disease. Therefore, there is a need for new and less toxic therapeutic agents. The aim of this study was to determine the antitumoral activity of omipalisib, a PI3K/AKT/mTOR pathway inhibitor, in the Burkitt lymphoma. Raji cell line was used in the study. Omipalisib was administered to the cell line and then the cytotoxic effect of omipalisib on Raji cells was evaluated by the XTT test. The IC50 value was calculated according to the results of the XTT test. Apoptosis and cell cycle experiments were studied with the calculated IC50 value. The flow cytometric method was used to determine the effect of omipalisib on apoptosis and cell death. The results of the study showed a statistically significant cytotoxic effect of increasing concentrations of omipalisib on Raji cells. The apoptosis experiment performed revealed that omipalisib strongly induced apoptosis. The cell cycle experiment showed that omipalisib stimulated the cell cycle arrest at the G0/G1 phase. It was concluded that omipalisib exhibited antitumoral activity on Burkitt lymphoma cells with its cytotoxic effect and induced apoptosis and cell cycle arrest. Considering this effect, targeting the PI3K/AKT/mTOR pathway with omipalisib can be a new treatment option.

Keywords

Supporting Institution

cübap

Project Number

T-896

Thanks

This study was supported by Sivas Cumhuriyet University Scientific Research Projects (CUBAP) with the project number of T-896. The authors thank Sivas Cumhuriyet University Medical Faculty Research Center-CUTFAM for supplying the required facilities for the conduct of this study.

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Zekeriya Keskin ^*
0000-0003-3623-9892
Türkiye

Fatih Yulak
0000-0003-3708-6752
Türkiye

Hatice Terzi
0000-0003-3471-1305
Türkiye

Merve İnanır
0000-0003-4661-8087
Türkiye

Publication Date

December 28, 2023

Submission Date

August 16, 2023

Acceptance Date

December 2, 2023

Published in Issue

Year 1970 Volume: 44 Number: 4

DOI

https://doi.org/10.17776/csj.1344535

IZ

https://izlik.org/JA74PY49PP

Cite

RIS / Bibtex

APA

Keskin, Z., Yulak, F., Terzi, H., & İnanır, M. (2023). Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line. Cumhuriyet Science Journal, 44(4), 635-639. https://doi.org/10.17776/csj.1344535

Öz

Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite