Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line

Fatih Yulak; Bünyamin Üngür

doi:10.17776/csj.1340869

EN

Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line

Abstract

Oxidative stress is defined as an imbalance between the generation of reactive oxygen species (ROS) and their scavenging. Indatralin, which has serotonin reuptake inhibitory activity, has not yet been studied for its ability to prevent oxidative damage. Our research's objective was to find out how indatraline defends against oxidative damage. C6 cells were used in the study and four different cell groups were created. The control group received no therapy at all. For 24 hours, cells in the H2O2 group were exposed to 0.5 mM H2O2. The indatraline group received indatraline treatments for 24 hours at various doses (0.5, 1, 2.5, 5 and 10 μM). For one hour, indatraline was administered to the indatraline + H2O2 group at various concentrations (0.5, 1, 2.5, 5 and 10 μM) before the group was subjected to 0.5 mM H2O2 for 24 hours. Following the occurrence of oxidative damage, total antioxidant status (TAS) and total oxidant status (TOS) levels were determined. Cell viability was also evaluated using the XTT assay. As a result, after hydrogen peroxide-induced oxidative damage, indatraline at doses of 10, 5, and 2.5 μM showed a protective effect by significantly enhanced cell survival in C6 cells(p < 0.001). Additionally, indatraline boosted the lowered TAS level while decreasing the elevated TOS levels following hydrogen peroxide-induced oxidative damage (p<0.001).

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012109251

Thanks

TÜBİTAK'a desteklerinden dolayı teşekkürlerimizi sunarız

References

[1] H. J. Forman, Use and abuse of exogenous H2O2 in studies of signal transduction, Free Radic. Biol. Med., vol. 42(7) (2007) 926–932.
[2] Karabulut H., Şükrü Gülay M., Serbest Radikaller., MAKÜ Sag. Bil. Enst. Derg., 4(41) (2016) 50–59.
[3] Kuraoka B., Robins P., Masutani C., Hanaoka F., Gasparutto D., Harbiyeli J., Ahşap RD., Lindahl T., Oxygen free radical damage to DNA. Translesion synthesis by human DNA polymerase eta and resistance to exonuclease action at cyclopurine deoxynucleoside residues., J. Biol. Chem., 276(52) (2001) 49283–49288.
[4] Devasagayam T. P. A., Boloor K. K., and Ramasarma T., Methods for estimating lipid peroxidation: an analysis of merits and demerits., Indian J. Biochem. Biophys., 40(5) (2003) 300–308.
[5] Bengal W., Free Radicals and Their Role in Different Clinical Conditions : An Overview, 1(3) (2010) 185–192.
[6] Devasagayam T. P. A., Tilak J. C., Boloor K. K., Sane K. S., Ghaskadbi S. S., and Lele R. D., Free radicals and antioxidants in human health: current status and future prospects., J. Assoc. Physicians India, 52(10) (2004) 794–804.
[7] Gandhi S. and Abramov A. Y., Mechanism of Oxidative Stress in Neurodegeneration, Oxid. Med. Cell. Longev., 2012 (2012) 428010. doi: 10.1155/2012/428010.
[8] Coyle J. T. and Puttfarcken P., Oxidative Stress, Glutamate, and Neurodegenerative Disorders, Science., 262(5134) (1993)n689–695.

[9] Shirley R., Ord E. N. J., and Work L. M., Oxidative Stress and the Use of Antioxidants in Stroke., Antioxidants, 3(3) (2014) 472–501.
[10] Dias V., Junn E., and Mouradian M. M., The role of oxidative stress in Parkinson’s disease., J. Parkinsons. Dis., 3(4) (2013) 461–491.
[11] Gella A. and Durany N., Oxidative stress in Alzheimer disease., Cell Adh. Migr., 3(1) (2009) 88–93.
[12] Jelinek M., Jurajda M., and Duris K., Oxidative Stress in the Brain: Basic Concepts and Treatment Strategies in Stroke., Antioxidants, vol. 10(12) (2021) 1886.
[13] Knapp L. T. and Klann E., Role of reactive oxygen species in hippocampal long-term potentiation: contributory or inhibitory?, J. Neurosci. Res., 70(1) (2002) 1–7.
[14] Zhuo M., Small S. A., Kandel E. R., and Hawkins R. D., Nitric oxide and carbon monoxide produce activity-dependent long-term synaptic enhancement in hippocampus., Science, 260(5116) 1946–1950.
[15] Rodrigo R., Fernández-Gajardo R., Gutierrez R. , Matamala J.M. , Carrasco R., Miranda-Merchak A., Feuerhake W., Oxidative stress and pathophysiology of ischemic stroke: novel therapeutic opportunities., CNS Neurol. Disord. Drug Targets, 12(5) (2013) 698–714.
[16] Bøgesø K. P., Christensen A. V, Hyttel J., and Liljefors T., 3-Phenyl-1-indanamines. Potential antidepressant activity and potent inhibition of dopamine, norepinephrine, and serotonin uptake., J. Med. Chem., 28(12) (1985) 1817–1828.
[17] Hyttel J. and Larsen J. J., Neurochemical profile of Lu 19-005, a potent inhibitor of uptake of dopamine, noradrenaline, and serotonin., J. Neurochem., 44(5) (1985) 1615–1622.
[18] Hunsberger J., Austin D. R., Henter I. D., and Chen G., The neurotrophic and neuroprotective effects of psychotropic agents., Dialogues Clin. Neurosci., 11(3) (2009) 333–348.
[19] Young L. T., Neuroprotective effects of antidepressant and mood stabilizing drugs., Journal of psychiatry & neuroscience : JPN, 27(1) (2002) 8–9.
[20] Cho Y. S., Yen C., Shim J. S., Kang D. H., and Kang S. W., Antidepressant indatraline induces autophagy and inhibits restenosis via suppression of mTOR / S6 kinase signaling pathway, Nat. Publ. Gr., 6(5) (2016) 1–9.
[21] Kruger N. J., The Bradford Method for Protein Quantitation BT - The Protein Protocols Handbook, Ed. Totowa, NJ: Humana Press, (2002)15–21.
[22] Erel O., A novel automated method to measure total antioxidant response against potent free radical reactions, Clin. Biochem., 37(2) (2004) 112–119.
[23] Erel O., A new automated colorimetric method for measuring total oxidant status, Clin. Biochem., 38(12) (2005) 1103–1111.
[24] Ergül M. and Taşkiran A. Ş., Thiamine protects glioblastoma cells against glutamate toxicity by suppressing oxidative/endoplasmic reticulum stress, Chem. Pharm. Bull., 69(9) (2021) 832–839.
[25] Armstrong R. W. and Wu C. C., Lattice Misorientation and Displaced Volume for Microhardness Indentations in MgO Crystals, J. Am. Ceram. Soc., 61(3–4) (1978) 102–106.
[26] Rao A. V and Balachandran B., Role of oxidative stress and antioxidants in neurodegenerative diseases., Nutr. Neurosci., 5(5) (2002) 291–309.
[27] Niedzielska E., Smaga I., Gawlik M., Moniczewski A., Stankowicz P., Pera J., Filip M., Oxidative Stress in Neurodegenerative Diseases., Mol. Neurobiol., 53(6) (2016) 4094–4125.
[28] Li J., O W., Li W., Jiang Z.-G., and Ghanbari H. A., Oxidative stress and neurodegenerative disorders., Int. J. Mol. Sci., 14(12) (2013) 24438–24475.
[29] Maher P. and Davis J. B., The role of monoamine metabolism in oxidative glutamate toxicity., J. Neurosci. Off. J. Soc. Neurosci., 16(20) (1996) 6394–6401.
[30] Nibuya M., Morinobu S., and Duman R. S., Regulation of BDNF and trkB mRNA in rat brain by chronic electroconvulsive seizure and antidepressant drug treatments., J. Neurosci. Off. J. Soc. Neurosci., 15(11) (1995) 7539–7547.
[31] Lindén A. M., Väisänen J., Lakso M., Nawa H., Wong G., and Castrén E., Expression of neurotrophins BDNF and NT-3, and their receptors in rat brain after administration of antipsychotic and psychotrophic agents., J. Mol. Neurosci., 14(1–2) (2000) 27–37.
[32] Hacısüleyman L., Saraç B., and Joha Z., Analgesic Effects of Vilazodone, Indatraline, and Talsupram in a Rat Model of Neuropathic Pain., Turkish J. Pharm. Sci., 19(3) (2021) 336–342.
[33] Abdel-Salam O. M. E., Morsy S. M. Y., and Sleem A. A., The effect of different antidepressant drugs on oxidative stress after lipopolysaccharide administration in mice., EXCLI J., 10 (2011) 290–302.
[34] Severcan S. M., Severcan C., Tazehkand M. N., and Oz Z. S., Evaluation of O xidant- A ntioxidant S tatus of F luvoxamine on H uman L ymphocyte C ell C ulture, 8(1) (2021) 79–83.

Details

Primary Language

English

Subjects

Toxicology

Journal Section

Research Article

Authors

Fatih Yulak ^*
0000-0003-3708-6752
Türkiye

Bünyamin Üngür
0009-0003-7635-6820
Türkiye

Publication Date

December 28, 2023

Submission Date

August 10, 2023

Acceptance Date

December 12, 2023

Published in Issue

Year 2023 Volume: 44 Number: 4

DOI

https://doi.org/10.17776/csj.1340869

IZ

https://izlik.org/JA24AF44FK

Cite

RIS / Bibtex

APA

Yulak, F., & Üngür, B. (2023). Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line. Cumhuriyet Science Journal, 44(4), 645-649. https://doi.org/10.17776/csj.1340869

Cited By

Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells

Cumhuriyet Science Journal

https://doi.org/10.17776/csj.1535242

Öz

Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells