Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells

Zıad Joha; Mustafa Ergül

doi:10.17776/csj.1336167

EN

Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells

Abstract

Previous studies have reported the anticancer properties of β-glucan on various cancer cells. The objective of this research was to investigate the involvement of apoptosis in the cytotoxic action of β-glucan on the A549 cells. The cytotoxic impact of this drug on A549 cells was examined by subjecting them to various quantities of the substance, and the XTT assay was utilized to determine cell survival. Flow cytometry was performed to investigate apoptosis. A statistically significant and dose-dependent cytotoxic impact on A549 cells was observed upon treatment with β-glucan. The calculated IC50 value of β-glucan for A549 cells after a 24-hour treatment period was discovered to be 82.16 μg/mL. Further investigations carried out using the IC50 dose of β-glucan revealed a significant increase in the late apoptotic cells percentage. The capacity of β-glucan to trigger apoptosis is thought to be the cause of its cytotoxic action on lung cancer. The revelation of this discovery emphasizes the promising possibilities of β-glucan as an effective therapeutic choice.

Keywords

Thanks

Acknowledgments: The 1Sivas 1Cumhuriyet University, 1School of Medicine, 1CUTFAM Research Center, Sivas, 1Turkey, provided the necessary tools and infrastructure 1for the execution of this examination, for which the authors are grateful.

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Zıad Joha ^*
0000-0001-8520-3760
Türkiye

Mustafa Ergül
0000-0003-4303-2996
Türkiye

Publication Date

December 28, 2023

Submission Date

August 1, 2023

Acceptance Date

December 2, 2023

Published in Issue

Year 2023 Volume: 44 Number: 4

DOI

https://doi.org/10.17776/csj.1336167

IZ

https://izlik.org/JA26MM96LW

Cite

RIS / Bibtex

APA

Joha, Z., & Ergül, M. (2023). Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells. Cumhuriyet Science Journal, 44(4), 640-644. https://doi.org/10.17776/csj.1336167

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International Journal of Molecular Sciences

https://doi.org/10.3390/ijms27031433

Öz

Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Characterization of β-(1→3)-D-glucan from the mutant strain BMA2 of Ustilago maydis: physicochemical, rheological, cytotoxic, antioxidant, and immunomodulatory properties for industrial applications

Immunotoxicity of Inhalable Organic Dust Samples Based on In Vitro Analysis of Human Respiratory Epithelial Cells