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Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells

Yıl 2023, Cilt: 44 Sayı: 4, 640 - 644, 28.12.2023

Zıad Joha Mustafa Ergül

https://doi.org/10.17776/csj.1336167

Öz

Previous studies have reported the anticancer properties of β-glucan on various cancer cells. The objective of this research was to investigate the involvement of apoptosis in the cytotoxic action of β-glucan on the A549 cells. The cytotoxic impact of this drug on A549 cells was examined by subjecting them to various quantities of the substance, and the XTT assay was utilized to determine cell survival. Flow cytometry was performed to investigate apoptosis. A statistically significant and dose-dependent cytotoxic impact on A549 cells was observed upon treatment with β-glucan. The calculated IC50 value of β-glucan for A549 cells after a 24-hour treatment period was discovered to be 82.16 μg/mL. Further investigations carried out using the IC50 dose of β-glucan revealed a significant increase in the late apoptotic cells percentage. The capacity of β-glucan to trigger apoptosis is thought to be the cause of its cytotoxic action on lung cancer. The revelation of this discovery emphasizes the promising possibilities of β-glucan as an effective therapeutic choice.

Anahtar Kelimeler

Anticancer β-glucan Apoptosis A549

Teşekkür

Acknowledgments: The 1Sivas 1Cumhuriyet University, 1School of Medicine, 1CUTFAM Research Center, Sivas, 1Turkey, provided the necessary tools and infrastructure 1for the execution of this examination, for which the authors are grateful.

Kaynakça

  • [1] Roudi R., Mohammadi S.R., Roudbary M, Mohsenzadegan M., Lung cancer and β-glucans: review of potential therapeutic applications, Investigational New Drugs, 35 (2017) 509–17.
  • [2] Peymaeei F., Sadeghi F., Safari E., Khorrami S., Falahati M., Mohammadi S.R., et al., Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study, Asian Pacific Journal of Cancer Prevention, 21 (2020) 837.
  • [3] Koparal A.T., Zeytinoglu M., Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549, Cytotechnology, 43 (2003) 149–54.
  • [4] Tong X., Tang R., Xiao M., Xu J., Wang W., Zhang B., et al., Targeting cell death pathways for cancer therapy: recent developments in necroptosis, pyroptosis, ferroptosis, and cuproptosis research, Journal of Hematology & Oncology, 15 (2022) 1–32.
  • [5] Castro-Cruz A., Echeverría O.M., Juárez-Chavero S., Sánchez-Sánchez L., Torres-Ramírez N., Vázquez-Nin G.H., et al., Transcriptional activity and splicing factors are preserved during physiological apoptosis, Journal of Structural Biology, 214 (2022) 107884.
  • [6] Liu Y., Chen Y., Lin L., Li H., Gambogic acid as a candidate for cancer therapy: A review, International Journal of Nanomedicine, 15 (2020) 10385–99.
  • [7] Thomas S., Rezoagli E., Abidin I.Z., Major I., Murray P., Murphy E.J., β-Glucans from Yeast—Immunomodulators from Novel Waste Resources, Applied Sciences, 12 (2022) 5208.
  • [8] Karimi R., Homayoonfal M., Malekjani N., Kharazmi M.S., Jafari S.M., Interaction between β-glucans and gut microbiota: a comprehensive review, Critical Reviews in Food Science and Nutrition, (2023) 1-32.
  • [9] Sima P., Richter J., Vetvicka V., Glucans as New Anticancer Agents, Anticancer Research, 39 (2019) 3373–8.
  • [10] Ikewaki N., Dedeepiya V.D., Raghavan K., Rao K.S., Vaddi S., Osawa H., et al. β‑glucan vaccine adjuvant approach for cancer treatment through immune enhancement (B‑VACCIEN) in specific immunocompromised populations (Review), Oncology Reports, 47 (2022) 1–9.
  • [11] Ergul M., Bakar-Ates F., A specific inhibitor of polo-like kinase 1, GSK461364A, suppresses proliferation of Raji Burkitt’s lymphoma cells through mediating cell cycle arrest, DNA damage, and apoptosis, Chemico-Biological Interactions, 332 (2020) 109288.
  • [12] Ergul M., Bakar-Ates F., Investigation of molecular mechanisms underlying the antiproliferative effects of colchicine against PC3 prostate cancer cells, Toxicology in Vitro, 73 (2021) 105138.
  • [13] Çiltaş A.Ç., Gündoğdu S., Yulak F., Levetiracetam Protects Against Glutamate-Induced Excitotoxicity in SH-SY5Y Cell Line, International Journal of Nature and Life Sciences, 6 (2022) 142–50.
  • [14] Bilal .S, Handan G., Aysegul O., Investigation of the mechanisms involved in anticancer effect of glucosamine sulfate on SH-SY5Y cell line, Bratislavske Lekarske Listy, 123 (2022) 366–71.
  • [15] Taskiran A.S., Ergul M., Gunes H., Ozturk A., Sahin B., Ozdemir E., The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line, Cellular and Molecular Neurobiology, 41 (2021) 173–83.
  • [16] Maimon Y., Karaush V., Yaal-Hahoshen N., Ben-Yosef R., Ron I., Vexler A., et al., Effect of Chinese Herbal Therapy on Breast Cancer Adenocarcinoma Cell Lines, Journal of International Medical Research, 38 (2010) 2033–9.
  • [17] Kim M.J., Hong S.Y., Kim S.K., Cheong C., Park H.J., Chun H.K., et al., β-Glucan enhanced apoptosis in human colon cancer cells SNU-C4, Nutrition Research and Practice, 3 (2009) 180–4.
  • [18] Vetvicka V., Vetvickova J., Glucans and Cancer: Comparison of Commercially Available β-glucans – Part IV, Anticancer Research, 38 (2018) 1327–33.
  • [19] Sadeghi F., Peymaeei F., Falahati M., Safari E., Farahyar S., Roudbar Mohammadi S, et al., The effect of Candida cell wall beta-glucan on treatment-resistant LL/2 cancer cell line: in vitro evaluation, Molecular Biology Reports, 47 (2020) 3653–61.
  • [20] Upadhyay T.K., Trivedi R., Khan F., Al-Keridis L.A., Pandey P., Sharangi A.B., et al., In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived β-1,3-glucan particles against cervical cancer cells, Frontiers in Oncology, 12 (2022) 942075.
  • [21] Filiz A.K., Joha Z., Yulak F., Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line, Bangladesh Journal of Pharmacology, 16 (2021) 122–8.
  • [22] D’Arcy M.S., Cell death: a review of the major forms of apoptosis, necrosis and autophagy, Cell Biology International, 43 (2019) 582–92.
  • [23] Van Heerde W.L., De Groot P.G., Reutelingsperger C.P.M., The complexity of the phospholipid binding protein annexin V, Thrombosis and Haemostasis, 73 (1995) 172–9.
  • [24] Chen Z., Zhang B., Gao F., Shi R., Modulation of G2/M cell cycle arrest and apoptosis by luteolin in human colon cancer cells and xenografts, Oncology Letters, 15 (2018) 1559–65.
  • [25] Xu H., Zou S., Xu X., Zhang L., Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism, Scientific Reports, 6 (2016) 1–13.
  • [26] Atiq A., Parhar I., Anti-neoplastic Potential of Flavonoids and Polysaccharide Phytochemicals in Glioblastoma, Molecules, 25 (2020) 4895.
  • [27] Zhang Y., Li Q., Wang J., Cheng F., Huang X., Cheng Y., et al., Polysaccharide from Lentinus edodes combined with oxaliplatin possesses the synergy and attenuation effect in hepatocellular carcinoma, Cancer Letters, 377 (2016) 117–25.
  • [28] Xu H.L., Dai J.H., Hu T., Liao Y.F., Lentinan up-regulates microRNA-340 to promote apoptosis and autophagy of human osteosarcoma cells, International Journal of Clinical and Experimental Pathology, 11 (2018) 3876.

Yıl 2023, Cilt: 44 Sayı: 4, 640 - 644, 28.12.2023

Zıad Joha Mustafa Ergül

https://doi.org/10.17776/csj.1336167

Öz

Kaynakça

  • [1] Roudi R., Mohammadi S.R., Roudbary M, Mohsenzadegan M., Lung cancer and β-glucans: review of potential therapeutic applications, Investigational New Drugs, 35 (2017) 509–17.
  • [2] Peymaeei F., Sadeghi F., Safari E., Khorrami S., Falahati M., Mohammadi S.R., et al., Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study, Asian Pacific Journal of Cancer Prevention, 21 (2020) 837.
  • [3] Koparal A.T., Zeytinoglu M., Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549, Cytotechnology, 43 (2003) 149–54.
  • [4] Tong X., Tang R., Xiao M., Xu J., Wang W., Zhang B., et al., Targeting cell death pathways for cancer therapy: recent developments in necroptosis, pyroptosis, ferroptosis, and cuproptosis research, Journal of Hematology & Oncology, 15 (2022) 1–32.
  • [5] Castro-Cruz A., Echeverría O.M., Juárez-Chavero S., Sánchez-Sánchez L., Torres-Ramírez N., Vázquez-Nin G.H., et al., Transcriptional activity and splicing factors are preserved during physiological apoptosis, Journal of Structural Biology, 214 (2022) 107884.
  • [6] Liu Y., Chen Y., Lin L., Li H., Gambogic acid as a candidate for cancer therapy: A review, International Journal of Nanomedicine, 15 (2020) 10385–99.
  • [7] Thomas S., Rezoagli E., Abidin I.Z., Major I., Murray P., Murphy E.J., β-Glucans from Yeast—Immunomodulators from Novel Waste Resources, Applied Sciences, 12 (2022) 5208.
  • [8] Karimi R., Homayoonfal M., Malekjani N., Kharazmi M.S., Jafari S.M., Interaction between β-glucans and gut microbiota: a comprehensive review, Critical Reviews in Food Science and Nutrition, (2023) 1-32.
  • [9] Sima P., Richter J., Vetvicka V., Glucans as New Anticancer Agents, Anticancer Research, 39 (2019) 3373–8.
  • [10] Ikewaki N., Dedeepiya V.D., Raghavan K., Rao K.S., Vaddi S., Osawa H., et al. β‑glucan vaccine adjuvant approach for cancer treatment through immune enhancement (B‑VACCIEN) in specific immunocompromised populations (Review), Oncology Reports, 47 (2022) 1–9.
  • [11] Ergul M., Bakar-Ates F., A specific inhibitor of polo-like kinase 1, GSK461364A, suppresses proliferation of Raji Burkitt’s lymphoma cells through mediating cell cycle arrest, DNA damage, and apoptosis, Chemico-Biological Interactions, 332 (2020) 109288.
  • [12] Ergul M., Bakar-Ates F., Investigation of molecular mechanisms underlying the antiproliferative effects of colchicine against PC3 prostate cancer cells, Toxicology in Vitro, 73 (2021) 105138.
  • [13] Çiltaş A.Ç., Gündoğdu S., Yulak F., Levetiracetam Protects Against Glutamate-Induced Excitotoxicity in SH-SY5Y Cell Line, International Journal of Nature and Life Sciences, 6 (2022) 142–50.
  • [14] Bilal .S, Handan G., Aysegul O., Investigation of the mechanisms involved in anticancer effect of glucosamine sulfate on SH-SY5Y cell line, Bratislavske Lekarske Listy, 123 (2022) 366–71.
  • [15] Taskiran A.S., Ergul M., Gunes H., Ozturk A., Sahin B., Ozdemir E., The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line, Cellular and Molecular Neurobiology, 41 (2021) 173–83.
  • [16] Maimon Y., Karaush V., Yaal-Hahoshen N., Ben-Yosef R., Ron I., Vexler A., et al., Effect of Chinese Herbal Therapy on Breast Cancer Adenocarcinoma Cell Lines, Journal of International Medical Research, 38 (2010) 2033–9.
  • [17] Kim M.J., Hong S.Y., Kim S.K., Cheong C., Park H.J., Chun H.K., et al., β-Glucan enhanced apoptosis in human colon cancer cells SNU-C4, Nutrition Research and Practice, 3 (2009) 180–4.
  • [18] Vetvicka V., Vetvickova J., Glucans and Cancer: Comparison of Commercially Available β-glucans – Part IV, Anticancer Research, 38 (2018) 1327–33.
  • [19] Sadeghi F., Peymaeei F., Falahati M., Safari E., Farahyar S., Roudbar Mohammadi S, et al., The effect of Candida cell wall beta-glucan on treatment-resistant LL/2 cancer cell line: in vitro evaluation, Molecular Biology Reports, 47 (2020) 3653–61.
  • [20] Upadhyay T.K., Trivedi R., Khan F., Al-Keridis L.A., Pandey P., Sharangi A.B., et al., In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived β-1,3-glucan particles against cervical cancer cells, Frontiers in Oncology, 12 (2022) 942075.
  • [21] Filiz A.K., Joha Z., Yulak F., Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line, Bangladesh Journal of Pharmacology, 16 (2021) 122–8.
  • [22] D’Arcy M.S., Cell death: a review of the major forms of apoptosis, necrosis and autophagy, Cell Biology International, 43 (2019) 582–92.
  • [23] Van Heerde W.L., De Groot P.G., Reutelingsperger C.P.M., The complexity of the phospholipid binding protein annexin V, Thrombosis and Haemostasis, 73 (1995) 172–9.
  • [24] Chen Z., Zhang B., Gao F., Shi R., Modulation of G2/M cell cycle arrest and apoptosis by luteolin in human colon cancer cells and xenografts, Oncology Letters, 15 (2018) 1559–65.
  • [25] Xu H., Zou S., Xu X., Zhang L., Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism, Scientific Reports, 6 (2016) 1–13.
  • [26] Atiq A., Parhar I., Anti-neoplastic Potential of Flavonoids and Polysaccharide Phytochemicals in Glioblastoma, Molecules, 25 (2020) 4895.
  • [27] Zhang Y., Li Q., Wang J., Cheng F., Huang X., Cheng Y., et al., Polysaccharide from Lentinus edodes combined with oxaliplatin possesses the synergy and attenuation effect in hepatocellular carcinoma, Cancer Letters, 377 (2016) 117–25.
  • [28] Xu H.L., Dai J.H., Hu T., Liao Y.F., Lentinan up-regulates microRNA-340 to promote apoptosis and autophagy of human osteosarcoma cells, International Journal of Clinical and Experimental Pathology, 11 (2018) 3876.
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Temel Farmakoloji
Bölüm Natural Sciences
Yazarlar

Zıad Joha 0000-0001-8520-3760

Mustafa Ergül 0000-0003-4303-2996

Yayımlanma Tarihi 28 Aralık 2023
Gönderilme Tarihi 1 Ağustos 2023
Kabul Tarihi 2 Aralık 2023
Yayımlandığı Sayı Yıl 2023Cilt: 44 Sayı: 4

Kaynak Göster

APA Joha, Z., & Ergül, M. (2023). Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells. Cumhuriyet Science Journal, 44(4), 640-644. https://doi.org/10.17776/csj.1336167
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