Total Phenolic Content, Free Radical Scavenging Activity and Antibacterial Activity of Some Buplerum Species

The aim of this research was to determine the total phenolic content, free radical scavenging activity and antibacterial activity of some Bupleurum species. In the study, twelve Bupleurum L. (Apiaceae) species were extracted using a mixture of methanol-water and tested for total phenolic content, free radical scavenging activity and antibacterial activity. Total phenolic content of Bupleurum species extracts ranged between 56.64 and 317.54 mg gallic acid equivalent (GAE)/g dry extract. The free radical scavenging activity of extracts was determined using the IC50 method, and the results for the samples ranged from 0.39 to 3.41 mg/ml. The antibacterial properties of Bupleurum species extracts were tested using the microdilution method against a total of eleven bacterial strains in this study; no inhibition was observed in the range of the investigated extract concentrations. Bupleurum species were contained phenolic compounds and had some free radical scavenging potential, but they did not show inhibitory impact on bacteria in the investigated extract concentration range. All the analyses were made in three replications. Results were provided as a mean of the replicates. The methods for results were looked at by utilizing the single direction and multivariate investigation of difference (MANOVA) followed by Duncan's different reach tests. The contrasts between singular methods were considered to be critical at P < 0.05.