Araştırma Makalesi
Yıl 2019,
Cilt: 40 Sayı: 1, 150 - 157, 22.03.2019
Öz
Bu çalışmada, bir
flavonoid olan baicalein’in MCF-7 ve MDA-MB-231 hücre hatları üzerinde meydana
getireceği antiproliferatif etkilerin karşılaştırılması amaçlanmıştır. Hücre
canlılığı, hücre indeksi, mitotik indeks, işaretlenme indeksi ve apoptotik indeks
gibi hücre kinetiği parametrelerinin değerlendirilmesi ile deneyler
gerçekleştirilmiştir.
Yapılan
hücre canlılığı testi ile baicalein’in
MCF-7 ve MDA-MB-231 hücreleri için IC50 konsantrasyonları sırasıyla
10 µM ve 30 µM olarak belirlenmiş ve tüm deneylerde bu konsantrasyonlar
kullanılmıştır. Elde edilen sonuçlar kullanılan IC50
konsantrasyonların her hücre tipi için de hücre canlılığı, hücre indeksi,
mitotik indeks ve işaretlenme indeksi değerlerini azaltırken apoptotik indeks
değerini yükselttiğini göstermiştir. Bu azalma ve artışlar istatistiksel olarak
anlamlıdır (p<0.01). Ayrıca xCelligence Gerçek Zamanlı Hücre Analiz
Sisteminden elde edilen grafiklere ait eğriler, baicalein’in MCF-7 hücrelerinde
sitoskeletal etkiler meydana getirirken MDA-MB-231 hücrelerinde sitostatik etki
meydana getirdiğini göstermiştir. Bu çalışmadan elde edilen sonuçlar baicalein’in
MCF-7 ve MDA-MB-231 hücreleri üzerine antiproliferatif etkilerinden dolayı
ileride yapılacak çalışmalarla desteklenerek, klinikte luminal A ve üçlü
negatif meme kanseri hastaları için de kullanılabileceğini düşündürmektedir.
Anahtar Kelimeler
Kaynakça
- [1]. Xie HY, Shao ZM and Li DQ., Tumor microenvironment: driving forces and potential therapeutic targets for breast cancer metastasis. Chin J. Cancer, 36 (2017) 36.
- [2]. Siegel RL, Miller KD and Jemal A., Cancer statistics, 2016. C.A. Cancer J Clin., 66 (2016) 7-30.
- [3]. Çetin İ, Topçul MR., Triple Negative Breast Cancer, APJCP. 15 (2014) 2427-243.
- [4]. Di Carlo G, Mascolo N, Izzo AA, Capasso F., Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci., 65 (1999) 337-353.
- [5]. Plaumann B, Fritsche M, Rimpler H, Brandner G, Hess RD., Flavonoids activate wild-type p53. Oncogene, 13 (1996) 1605-1614.
- [6]. Naasani I, Oh-Hashi F, Oh-Hara T, et al., Blocking telomerase by dietary polyphenols is a major mechanism for limiting the growth of human cancer cells in vitro and in vivo. Cancer, 63 (2003) 824-830.
- [7]. Kobayashi T, Nakata T, Kuzumaki T., Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Lett., 176 (2002) 17-23.
- [8]. Bonham M, Posakony J, Coleman I, Montgomery B, Simon J, Nelson PS., Characterization of chemical constituents in Scutellaria baicalensis with antiandrogenic and growth-inhibitory activities toward prostate carcinoma. Clin Cancer Res., 11 (2005) 3905-3914.
- [9]. Miocinovic R, McCabe NP, Keck RW, Jankun J, Hampton JA, Selman SH., In vivo and in vitro effect of baicalein on human prostate cancer cells. Int J. Oncol., 26 (2005) 241-246. [10]. Ma Z, Otsuyama K, Liu S, et al., Baicalein, a component of Scutellaria radix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells. Blood, 105 (2005) 3312- 3318.
- [11]. Lee HZ, Leung HWC, Lai MY, Wu CH., Baicalein induced cell cycle arrest and apoptosis in human lung squamous carcinoma CH27 cells. Anticancer Res. 25 (2005) 959-964.
- [12]. Chao JI, Su WC, Liu HF., Baicalein induces cancer cell death and proliferation retardation by the inhibition of CDC2 kinase and survivin associated with opposite role of p38 mitogen-activated protein kinase and AKT. Mol Cancer Ther., 6 (2007) 3039-3048.
- [13]. Chen Z, Hou R, Gao S, Song D, Feng Y., Baicalein inhibits proliferation activity of human colorectal cancer cells HCT116 through downregulation of ezrin. Cell Physiol Biochem., 49 (2018) 2035-2046.
- [14]. Mu J, Liu T, Jiang L, Wu X, Cao Y, Li M, Dong Q, Liu Y, Xu H., The Traditional Chinese medicine baicalein potently inhibits gastric cancer cells. Journal of Cancer, 7 (2016) 453-461.
- [15]. Lin CW, Yang LY, Shen SC and Chen YC., IGF-I plus E2 induces proliferation via activation of ROS-dependent ERKs and JNKs in human breast carcinoma cells. J Cell Physiol., 2012 (2007) 666-674.
- [16]. Branham WS, Dial SL, Moland CL, Hass BS, Blair RM, Fang H, Shi L, Tong W, Perkins RG and Sheehan DM., Phytoestrogens and mycoestrogens bind to the rat uterine estrogen receptor. J Nutr., 132 (2002) 658-664.
- [17]. Shenouda NS, Zhou C, Browning JD, Ansell PJ, Sakla MS, Lubahn DB and Macdonald RS., Phytoestrogens in common herbs regulate prostate cancer cell growth in vitro. Nutr Cancer, 49 (2004) 200-208.
- [18]. Po LS, Chen ZY, Tsang DS, Leung LK., Baicalein and genistein display differential actions on estrogen receptor (ER) transactivation and apoptosis in MCF-7 cells. Cancer Lett., 187 (2002) 33-40.
- [19]. An H, Yu X, Xiang C, Zhang Y, Xia J, Wang Y., Original Article Baicalein and U0126 suppress human breast cancer cell line MCF-7 through regulating MAPK signaling pathway. Int J Clin Exp Pathol., 9 (2016) 10266-10273.
- [20]. Ma X, Yan W, Dai Z, Gao X, Ma Y, Xu Q, Jiang J, Zhang S., Baicalein suppresses metastasis of breast cancer cells by inhibiting EMT via downregulation of SATB1 and Wnt/β-catenin pathway. Drug Design, Development and Therapy, 10 (2016) 1419-1441.
Yıl 2019,
Cilt: 40 Sayı: 1, 150 - 157, 22.03.2019
Öz
The aim of this study was to compare the antiproliferative effects of
baicalein which is a flavonoide on MCF-7 and MDA-MB-231 cell line. The
experiments were carried out with the evaluation of the parameters. Including
cell viability, cell index, mitotic index, labelling index and apoptotic index.
With the cell viability test, IC50 concentrations of baicalein for
MCF-7 and MDA-MB-231 cells were determined as 10 µM and 30 µM, respectively and
these concentrations were used in all experiments. The results showed that the
IC50 concentrations decreased the values of cell viability, cell
index, mitotic index and labelling index and increased the apoptotic index
value for both cell types. These decreases and increases are statistically
significant (p<0.01). In addition, curves of the graphs obtained from the
xCelligence Real-Time Cell Analysis System showed that baicalein has
cytoskeletal effects on MCF-7 cells and cytostatic effects on MDA-MB-231 cells.
The results obtained from this study suggest that baicalein can be used in
patients with luminal A and triple negative breast cancer in the clinic by
supporting the future studies due to its antiproliferative effects on MCF-7 and
MDA-MB-231 cells.
baicalein which is a flavonoide on MCF-7 and MDA-MB-231 cell line. The
experiments were carried out with the evaluation of the parameters. Including
cell viability, cell index, mitotic index, labelling index and apoptotic index.
With the cell viability test, IC50 concentrations of baicalein for
MCF-7 and MDA-MB-231 cells were determined as 10 µM and 30 µM, respectively and
these concentrations were used in all experiments. The results showed that the
IC50 concentrations decreased the values of cell viability, cell
index, mitotic index and labelling index and increased the apoptotic index
value for both cell types. These decreases and increases are statistically
significant (p<0.01). In addition, curves of the graphs obtained from the
xCelligence Real-Time Cell Analysis System showed that baicalein has
cytoskeletal effects on MCF-7 cells and cytostatic effects on MDA-MB-231 cells.
The results obtained from this study suggest that baicalein can be used in
patients with luminal A and triple negative breast cancer in the clinic by
supporting the future studies due to its antiproliferative effects on MCF-7 and
MDA-MB-231 cells.
Anahtar Kelimeler
Baicalein Luminal A breast cancer Triple negative breast cancer
Kaynakça
- [1]. Xie HY, Shao ZM and Li DQ., Tumor microenvironment: driving forces and potential therapeutic targets for breast cancer metastasis. Chin J. Cancer, 36 (2017) 36.
- [2]. Siegel RL, Miller KD and Jemal A., Cancer statistics, 2016. C.A. Cancer J Clin., 66 (2016) 7-30.
- [3]. Çetin İ, Topçul MR., Triple Negative Breast Cancer, APJCP. 15 (2014) 2427-243.
- [4]. Di Carlo G, Mascolo N, Izzo AA, Capasso F., Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci., 65 (1999) 337-353.
- [5]. Plaumann B, Fritsche M, Rimpler H, Brandner G, Hess RD., Flavonoids activate wild-type p53. Oncogene, 13 (1996) 1605-1614.
- [6]. Naasani I, Oh-Hashi F, Oh-Hara T, et al., Blocking telomerase by dietary polyphenols is a major mechanism for limiting the growth of human cancer cells in vitro and in vivo. Cancer, 63 (2003) 824-830.
- [7]. Kobayashi T, Nakata T, Kuzumaki T., Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Lett., 176 (2002) 17-23.
- [8]. Bonham M, Posakony J, Coleman I, Montgomery B, Simon J, Nelson PS., Characterization of chemical constituents in Scutellaria baicalensis with antiandrogenic and growth-inhibitory activities toward prostate carcinoma. Clin Cancer Res., 11 (2005) 3905-3914.
- [9]. Miocinovic R, McCabe NP, Keck RW, Jankun J, Hampton JA, Selman SH., In vivo and in vitro effect of baicalein on human prostate cancer cells. Int J. Oncol., 26 (2005) 241-246. [10]. Ma Z, Otsuyama K, Liu S, et al., Baicalein, a component of Scutellaria radix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells. Blood, 105 (2005) 3312- 3318.
- [11]. Lee HZ, Leung HWC, Lai MY, Wu CH., Baicalein induced cell cycle arrest and apoptosis in human lung squamous carcinoma CH27 cells. Anticancer Res. 25 (2005) 959-964.
- [12]. Chao JI, Su WC, Liu HF., Baicalein induces cancer cell death and proliferation retardation by the inhibition of CDC2 kinase and survivin associated with opposite role of p38 mitogen-activated protein kinase and AKT. Mol Cancer Ther., 6 (2007) 3039-3048.
- [13]. Chen Z, Hou R, Gao S, Song D, Feng Y., Baicalein inhibits proliferation activity of human colorectal cancer cells HCT116 through downregulation of ezrin. Cell Physiol Biochem., 49 (2018) 2035-2046.
- [14]. Mu J, Liu T, Jiang L, Wu X, Cao Y, Li M, Dong Q, Liu Y, Xu H., The Traditional Chinese medicine baicalein potently inhibits gastric cancer cells. Journal of Cancer, 7 (2016) 453-461.
- [15]. Lin CW, Yang LY, Shen SC and Chen YC., IGF-I plus E2 induces proliferation via activation of ROS-dependent ERKs and JNKs in human breast carcinoma cells. J Cell Physiol., 2012 (2007) 666-674.
- [16]. Branham WS, Dial SL, Moland CL, Hass BS, Blair RM, Fang H, Shi L, Tong W, Perkins RG and Sheehan DM., Phytoestrogens and mycoestrogens bind to the rat uterine estrogen receptor. J Nutr., 132 (2002) 658-664.
- [17]. Shenouda NS, Zhou C, Browning JD, Ansell PJ, Sakla MS, Lubahn DB and Macdonald RS., Phytoestrogens in common herbs regulate prostate cancer cell growth in vitro. Nutr Cancer, 49 (2004) 200-208.
- [18]. Po LS, Chen ZY, Tsang DS, Leung LK., Baicalein and genistein display differential actions on estrogen receptor (ER) transactivation and apoptosis in MCF-7 cells. Cancer Lett., 187 (2002) 33-40.
- [19]. An H, Yu X, Xiang C, Zhang Y, Xia J, Wang Y., Original Article Baicalein and U0126 suppress human breast cancer cell line MCF-7 through regulating MAPK signaling pathway. Int J Clin Exp Pathol., 9 (2016) 10266-10273.
- [20]. Ma X, Yan W, Dai Z, Gao X, Ma Y, Xu Q, Jiang J, Zhang S., Baicalein suppresses metastasis of breast cancer cells by inhibiting EMT via downregulation of SATB1 and Wnt/β-catenin pathway. Drug Design, Development and Therapy, 10 (2016) 1419-1441.
Toplam 19 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Natural Sciences |
| Yazarlar | |
| Yayımlanma Tarihi | 22 Mart 2019 |
| Gönderilme Tarihi | 10 Ocak 2019 |
| Kabul Tarihi | 25 Şubat 2019 |
| Yayımlandığı Sayı | Yıl 2019Cilt: 40 Sayı: 1 |