Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines

Mehmet Rıfkı Topçul

doi:10.17776/csj.511141

TR EN

Baicalein’in Farklı Meme Kanseri Hücre hatlarındaki Kinetik Etkilerinin Değerlendirilmesi

Öz

Bu çalışmada, bir flavonoid olan baicalein’in MCF-7 ve MDA-MB-231 hücre hatları üzerinde meydana getireceği antiproliferatif etkilerin karşılaştırılması amaçlanmıştır. Hücre canlılığı, hücre indeksi, mitotik indeks, işaretlenme indeksi ve apoptotik indeks gibi hücre kinetiği parametrelerinin değerlendirilmesi ile deneyler gerçekleştirilmiştir.

Yapılan hücre canlılığı testi ile baicalein’in MCF-7 ve MDA-MB-231 hücreleri için IC₅₀ konsantrasyonları sırasıyla 10 µM ve 30 µM olarak belirlenmiş ve tüm deneylerde bu konsantrasyonlar kullanılmıştır. Elde edilen sonuçlar kullanılan IC₅₀ konsantrasyonların her hücre tipi için de hücre canlılığı, hücre indeksi, mitotik indeks ve işaretlenme indeksi değerlerini azaltırken apoptotik indeks değerini yükselttiğini göstermiştir. Bu azalma ve artışlar istatistiksel olarak anlamlıdır (p<0.01). Ayrıca xCelligence Gerçek Zamanlı Hücre Analiz Sisteminden elde edilen grafiklere ait eğriler, baicalein’in MCF-7 hücrelerinde sitoskeletal etkiler meydana getirirken MDA-MB-231 hücrelerinde sitostatik etki meydana getirdiğini göstermiştir. Bu çalışmadan elde edilen sonuçlar baicalein’in MCF-7 ve MDA-MB-231 hücreleri üzerine antiproliferatif etkilerinden dolayı ileride yapılacak çalışmalarla desteklenerek, klinikte luminal A ve üçlü negatif meme kanseri hastaları için de kullanılabileceğini düşündürmektedir.

Anahtar Kelimeler

Baicalein,Luminal A meme kanseri,Üçlü negatif meme kanseri

Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines

Abstract

The aim of this study was to compare the antiproliferative effects of baicalein which is a flavonoide on MCF-7 and MDA-MB-231 cell line. The experiments were carried out with the evaluation of the parameters. Including cell viability, cell index, mitotic index, labelling index and apoptotic index. With the cell viability test, IC₅₀ concentrations of baicalein for MCF-7 and MDA-MB-231 cells were determined as 10 µM and 30 µM, respectively and these concentrations were used in all experiments. The results showed that the IC₅₀ concentrations decreased the values of cell viability, cell index, mitotic index and labelling index and increased the apoptotic index value for both cell types. These decreases and increases are statistically significant (p<0.01). In addition, curves of the graphs obtained from the xCelligence Real-Time Cell Analysis System showed that baicalein has cytoskeletal effects on MCF-7 cells and cytostatic effects on MDA-MB-231 cells. The results obtained from this study suggest that baicalein can be used in patients with luminal A and triple negative breast cancer in the clinic by supporting the future studies due to its antiproliferative effects on MCF-7 and MDA-MB-231 cells.

Keywords

Baicalein,Luminal A breast cancer,Triple negative breast cancer

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Mehmet Rıfkı Topçul ^*
0000-0002-9685-9404
Türkiye

Publication Date

March 22, 2019

Submission Date

January 10, 2019

Acceptance Date

February 25, 2019

Published in Issue

Year 2019 Volume: 40 Number: 1

DOI

https://doi.org/10.17776/csj.511141

IZ

https://izlik.org/JA25FL26MN

Cite

RIS / Bibtex

APA

Topçul, M. R. (2019). Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines. Cumhuriyet Science Journal, 40(1), 150-157. https://doi.org/10.17776/csj.511141

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Pharmacy Reports

https://doi.org/10.51511/pr.26