In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action. Thus, studies to examine interactions with DNA using biosensors have gained momentum. In our study, it was investigated that Capecitabine (CPT), an anticancer drug, and glassy carbon electrode (GCE) interaction by using electrochemical methods. The interaction of CPT with calf thymus DNAs (dsDNA, ssDNA) immobilized on the electrode surface was analyzed by exploiting changes in the oxidation signals of the guanine base. The immobilization of DNA on the electrode surface has been optimized. Optimal DNA concentration and optimal interaction times were found. Electrochemical impedance spectroscopy technique was used for impedimetric measurements.
The results obtained confirmed that the ct-DNAs were immobilized on the electrode surface. Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.
DNA biosensors Capecitabine (CPT) Glassy carbon paste electrode (GCE) Differential pulse voltammetry (DPV) Electrochemical impedance spectroscopy (EIS).
Birincil Dil | İngilizce |
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Konular | Eczacılık ve İlaç Bilimleri |
Bölüm | Natural Sciences |
Yazarlar | |
Yayımlanma Tarihi | 29 Aralık 2021 |
Gönderilme Tarihi | 17 Haziran 2021 |
Kabul Tarihi | 3 Ekim 2021 |
Yayımlandığı Sayı | Yıl 2021Cilt: 42 Sayı: 4 |