Some Factors Affecting Free and Immobilized Amylase Activity

Taner Şahutoğlu; Derya Kızıloluk

doi:10.17776/csj.1549149

EN

Some Factors Affecting Free and Immobilized Amylase Activity

Abstract

In our study, we prepared chitosan beads and immobilized bacterial amylase enzyme on these beads; Parameters such as comparing the optimization conditions (pH, pH stability, temperature, thermal stability, substrate concentration) of free bacterial amylase and amylase immobilized on chitosan beads and investigating the effect of immobilization in its usage areas are included. The immobilized enzyme showed better activity at both lower and higher pH levels compared to the free enzyme. When considering pH stability, the results were consistent with the pH activity values. In other words, both free and immobilized amylase exhibited the highest activity at the same pH levels. However, the free amylase showed less stability at low and high pH levels compared to the immobilized amylase. In terms of temperature-dependent activity, it was found that both free and immobilized enzymes showed the best activity at the same temperature. However, the immobilized enzyme showed activity at higher temperatures than the free enzyme. Moreover, when studying the effect of substrate concentration on free and immobilized enzymes, both types of enzymes exhibited the best activity in the same concentration of starch solution. In this study, factors affecting immobilized and free amylase activity were examined.

Keywords

References

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Details

Primary Language

English

Subjects

Enzymes , Bioassays

Journal Section

Research Article

Authors

Taner Şahutoğlu
0009-0005-3206-2980
Türkiye

Derya Kızıloluk ^*
0000-0002-0896-6550
Türkiye

Publication Date

March 25, 2025

Submission Date

September 13, 2024

Acceptance Date

March 12, 2025

Published in Issue

Year 2025 Volume: 46 Number: 1

DOI

https://doi.org/10.17776/csj.1549149

IZ

https://izlik.org/JA57CA53DG

Cite

RIS / Bibtex

APA

Şahutoğlu, T., & Kızıloluk, D. (2025). Some Factors Affecting Free and Immobilized Amylase Activity. Cumhuriyet Science Journal, 46(1), 91-97. https://doi.org/10.17776/csj.1549149

Öz

Some Factors Affecting Free and Immobilized Amylase Activity

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite