Research Article
BibTex RIS Cite

Investigation of Antioxidant Effect of Origanum Hypericifolium Extracts in Different Solvents

Year 2025, Volume: 46 Issue: 1, 1 - 7, 25.03.2025

Muhammed Sufi , Ceylan Özsoy , Mehmet Çiçek

https://doi.org/10.17776/csj.1546844

Abstract

Origanum hypericifolium is one of the most widely distributed endemic species of the family Lamiaceae. Origanium hypericifolium contains mostly monoterpenes. Monoterpenes are volatile compounds with 10 carbons and are widely found in the plant kingdom. Monoterpenes, which are used as fragrances in perfumes and foodstuffs, have anti-fungal, anti-bacterial, anti-oxidant and anti-cancer effects.Various in vitro assays can be used to determine the antioxidant activity of Origanium hypericifolium extracts. In our study, total polyphenolic, total flavonoid content, iron reducing power, cupric ion reducing capacity and radical scavenging activity were evaluated to the antioxidant properties of various extracts of Origanum hypericifolium. TAS and TOS levels were also analyzed. The highest DPPH radical scavenging acitiviy value was 3.09±0.01 µg/mL in ethanol extract, Total polyphenol 144.00±1.89 µg GAE /g in ethanol extract, Total polyphenol 17. 08±0.85 µg CE /g in ethanol extract, FRAP 942.46±1.89 µg TE /g ethyl acetate and CUPRAC 2.54±0.01 mmol TE/g ethyl acetate extract.

Keywords

Antioxidants Free radicals Origanum hypericifolium

References

  • [1] Başer K.H.C.,(2002). The Turkish Origanum Species, In:Oregano, The Genera Origanum and Lippia, Ed.: S.E.Kintzios, Taylor and Francis, UK.
  • [2] Başer K.H.C., Tümen G., Özek T. ve Kürkçüoğlu M., Composition of the Essential Oil of Origanum sipyleum of Turkish Origin, Journal of Essential Oil Research, 4(1) (1992)139-142.
  • [3] Davis P.H., (1982). Flora of Turkey and the East Aegean Islands, vol: 7. Edinburg University Press, Edinburgh.
  • [4] Baytop T. (1999). Türkiye'de bitkiler ile tedavi (Geçmişte ve Bugün). İstanbul Üniversitesi Yayınları, İstanbul, Türkiye, 520s.
  • [5] Baytop T., (1994).Türkçe Bitki Adları Sözlüğü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, Türk Dil Kurumu Yayınları: 578, s.33, 206, Ankara.
  • [6] Kütükcü Z.M. (2016). Deneysel diyabetik dişi sıçan genital sisteminde Origanum Hyperıcıfolıum Esansiyel yağının etkilerinin ince yapı düzeyinde araştırılması (Master's thesis, Pamukkale Üniversitesi Sağlık Bilimleri Enstitüsü).
  • [7] Sonmez C., Effect of different harvest times on some yield and essential oil characteristics in Origanum onites L. Turkish Journal Of Field Crops, 24(1) (2019)106-110.
  • [8] Marino M., Bersani C., Comi G., Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International journal of food microbiology, 67(3) (2001) 187-195.
  • [9] Özcan M, Erkmen O,, Antimicrobial activity of the essential oils of Turkish plant spices, European Food Research and Technology, 212(6) (2001) 658-660.
  • [10] Topçu G., Bioactive triterpenoids from Salvia species. J Nat Prod, 69 (2006) 482–487.
  • [11] Tutar U., Hepokur C., Misir S., Hepokur A.I., DUMAN F., Antimicrobial, Antioxidant, Cytotoxic and Wound Healing Effects of Thymbra sintenisii Extract. Indian Journal of Pharmaceutical Sciences, 80(5) (2018).
  • [12] Gök H. N., Luca S. V., Ay S.T., Komsta Ł., Salmas R. E., Orhan I. E.,Skalicka-Woźniak K., Profiling the annual change of the neurobiological and antioxidant effects of five Origanum species in correlation with their phytochemical composition. Food Chemistry, 368 (2022) 130775.
  • [13] Cuendet M., Potterat O., Salvi A., Testa B.,Hostettmann K., A stilbene and dihydrochalcones with radical scavenging activities from Loiseleuria procumbens. Phytochemistry, 54(8) (2000) 871-874.
  • [14] Berköz M., Yalın S., Normal ve preeklamptik gebelerde lipid peroksidasyonu ve antioksidan aktivite. ADÜ Tıp Fakültesi Dergisi 10 (2009) 52-58.
  • [15] Dinçer Y., Akçay T., DNA Hasarı. Türk Biyokimya Dergisi,25(2) (2000)73-79.
  • [16] Lima C.F., Fernandes-Ferreira M., Pereira-Wilson C. Phenolic compounds protect HepG2 cells from oxidative damage: Relevance of glutathione levels. Life Sciences, 79 (2006)2056-2068.
  • [17] Chang C.C., Yang M.H., Wen H.M., Chern J.C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal, 10(3) (2002)178-82
  • [18] Yu L., Haley S., Perret J., Harris M., Wilson J., Qıan M., Free radical scavenging properties of wheat extracts. J Agric Food Chem, 50(6)(2002)1619-24.
  • [19] Arkan T. (2011). Daphne oleoides subsp. oleoides ve Daphne sericea'nın farklı çözücülerle antioksidan özellikleri (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • [20] Erel O., A novel automated direct measurement method fortotal antioxidant capacity using a new generation, morestable ABTS radical cation. Clin Biochem, 37(2004) 277–285.
  • [21] Erel O.,A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38 (2005)1103–1111
  • [22] Emine F., (2013). Isparta ve Afyon illerinde tıbbi ve aromatik amaçlı kullanılan bitkilerin satılma, toplanma ve kullanılma durumları. Doktora tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü.
  • [23] Azize T.,(2010). Türkiye’de yetişen Orıganum L. (Labıatae) cinsi üzerinde epidermal incelemeler. Yüksek lisans tezi. İnönü Üniversitesi Sağlık Bilimler Enstitüsü.
  • [24] Jin D., Mumper R.J., Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10) (2010)7313-52.
  • [25] Okan O. T., Varlıbaş H., Mehmet Ö.Z., Deniz İ., Antioksidan analiz yöntemleri ve doğu Karadeniz bölgesinde antioksidan kaynağı olarak kullanılabilecek odun dışı bazı bitkisel ürünler. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 13(1) (2013) 48-59.
  • [26] Hossain M.B., Brunton N.P., Patras A., Tiwari B., O’donnell C.P., Martin-Diana A.B., Barry-Ryan C., Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology. Ultrasonics sonochemistry, 19(3) (2012)582-590.
  • [27] Sökmen M., Serkedjieva J., Daferera D., Gulluce M., Polissiou M., Tepe B., Sokmen A., from herbal parts and callus cultures of Origanum acutidens. Journal of agricultural and food chemistry, 52(11)(2004) 3309-3312.
  • [28] Roby M. H. H., Sarhan M. A., Selim K.A.H., Khalel K.I. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Industrial Crops and Products, 43 (2013) 827-831.
  • [29] Celik A., Nur Herken E., Arslan İ., Zafer Özel M.,Mercan N. Screening of the constituents, antimicrobial and antioxidant activity of endemic Origanum hypericifolium O. Schwartz & PH Davis. Natural product research, 24(16) (2010)1568-1577.
  • [30] Fakir H., Us A.A., Sagdic M.,Tornuk F.,Essential Oil Composition, Antimicrobial and Bioactive Properties of Origanuim hypericifolium, An Endemic Plant Species grown in Turkey. Research Journal of Biotechnology,10, 11(2015).
  • [31] Cervato G., Carabelli M., Gervasio S., Cittera A., Cazzola R.,Cestaro B., Antioxbdant properties of oregano (Origanum vulgare) leaf extracts. Journal of Food Biochemistry, 24(6) (2000). 453-465.
  • [32] Özkan G. (2007). Türkiye'de Lamiaceae (Labiatae) familyasına ait baharat veya çeşni olarak kullanılan bazı bitkilerin fenolik bileşenleri ile antioksidan ve antimikrobiyal etkilerinin belirlenmesi (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • [33] Kaurinovic B., Popovic M., Vlaisavljevic S., Trivic S. Antioxidant capacity of Ocimum basilicum L. and Origanum vulgare L. extracts. Molecules, 16(9) (2011) 7401-7414.
  • [34] Baddal M.K. (2010). Origanum Minutiflorum bitkisinin antioksidan özelliklerinin incelenmesi (Doctoral dissertation, SDÜ Fen Bilimleri Enstitüsü).
  • [35] Sarikurkcu C., Zengin G., Oskay M., Uysal S., Ceylan R., Aktumsek A., Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Industrial Crops and Products, 70 (2015)178-184.
  • [36] Yılmaz H., Çarıkçı S., Kılıç T., Dirmenci T., Arabacı T., Gören A.C., Screening of chemical composition, antioxidant and anticholinesterase activity of section Brevifilamentum of Origanum (L.) species. Records of Natural Products, 11(5) (2017).
  • [37] Elhardallou S.B., Cytotoxicity and biological activity of selected Sudanese medicinal plants. Research Journal of Medicinal Plant, 5(3) (2011) 201-29.
  • [38] Taraphdar A.K., Roy M., Bhattacharya R.K. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention, Curr Sci, 80 (2001) 1387-1396.

Year 2025, Volume: 46 Issue: 1, 1 - 7, 25.03.2025

Muhammed Sufi , Ceylan Özsoy , Mehmet Çiçek

https://doi.org/10.17776/csj.1546844

Abstract

References

  • [1] Başer K.H.C.,(2002). The Turkish Origanum Species, In:Oregano, The Genera Origanum and Lippia, Ed.: S.E.Kintzios, Taylor and Francis, UK.
  • [2] Başer K.H.C., Tümen G., Özek T. ve Kürkçüoğlu M., Composition of the Essential Oil of Origanum sipyleum of Turkish Origin, Journal of Essential Oil Research, 4(1) (1992)139-142.
  • [3] Davis P.H., (1982). Flora of Turkey and the East Aegean Islands, vol: 7. Edinburg University Press, Edinburgh.
  • [4] Baytop T. (1999). Türkiye'de bitkiler ile tedavi (Geçmişte ve Bugün). İstanbul Üniversitesi Yayınları, İstanbul, Türkiye, 520s.
  • [5] Baytop T., (1994).Türkçe Bitki Adları Sözlüğü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, Türk Dil Kurumu Yayınları: 578, s.33, 206, Ankara.
  • [6] Kütükcü Z.M. (2016). Deneysel diyabetik dişi sıçan genital sisteminde Origanum Hyperıcıfolıum Esansiyel yağının etkilerinin ince yapı düzeyinde araştırılması (Master's thesis, Pamukkale Üniversitesi Sağlık Bilimleri Enstitüsü).
  • [7] Sonmez C., Effect of different harvest times on some yield and essential oil characteristics in Origanum onites L. Turkish Journal Of Field Crops, 24(1) (2019)106-110.
  • [8] Marino M., Bersani C., Comi G., Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International journal of food microbiology, 67(3) (2001) 187-195.
  • [9] Özcan M, Erkmen O,, Antimicrobial activity of the essential oils of Turkish plant spices, European Food Research and Technology, 212(6) (2001) 658-660.
  • [10] Topçu G., Bioactive triterpenoids from Salvia species. J Nat Prod, 69 (2006) 482–487.
  • [11] Tutar U., Hepokur C., Misir S., Hepokur A.I., DUMAN F., Antimicrobial, Antioxidant, Cytotoxic and Wound Healing Effects of Thymbra sintenisii Extract. Indian Journal of Pharmaceutical Sciences, 80(5) (2018).
  • [12] Gök H. N., Luca S. V., Ay S.T., Komsta Ł., Salmas R. E., Orhan I. E.,Skalicka-Woźniak K., Profiling the annual change of the neurobiological and antioxidant effects of five Origanum species in correlation with their phytochemical composition. Food Chemistry, 368 (2022) 130775.
  • [13] Cuendet M., Potterat O., Salvi A., Testa B.,Hostettmann K., A stilbene and dihydrochalcones with radical scavenging activities from Loiseleuria procumbens. Phytochemistry, 54(8) (2000) 871-874.
  • [14] Berköz M., Yalın S., Normal ve preeklamptik gebelerde lipid peroksidasyonu ve antioksidan aktivite. ADÜ Tıp Fakültesi Dergisi 10 (2009) 52-58.
  • [15] Dinçer Y., Akçay T., DNA Hasarı. Türk Biyokimya Dergisi,25(2) (2000)73-79.
  • [16] Lima C.F., Fernandes-Ferreira M., Pereira-Wilson C. Phenolic compounds protect HepG2 cells from oxidative damage: Relevance of glutathione levels. Life Sciences, 79 (2006)2056-2068.
  • [17] Chang C.C., Yang M.H., Wen H.M., Chern J.C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal, 10(3) (2002)178-82
  • [18] Yu L., Haley S., Perret J., Harris M., Wilson J., Qıan M., Free radical scavenging properties of wheat extracts. J Agric Food Chem, 50(6)(2002)1619-24.
  • [19] Arkan T. (2011). Daphne oleoides subsp. oleoides ve Daphne sericea'nın farklı çözücülerle antioksidan özellikleri (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • [20] Erel O., A novel automated direct measurement method fortotal antioxidant capacity using a new generation, morestable ABTS radical cation. Clin Biochem, 37(2004) 277–285.
  • [21] Erel O.,A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38 (2005)1103–1111
  • [22] Emine F., (2013). Isparta ve Afyon illerinde tıbbi ve aromatik amaçlı kullanılan bitkilerin satılma, toplanma ve kullanılma durumları. Doktora tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü.
  • [23] Azize T.,(2010). Türkiye’de yetişen Orıganum L. (Labıatae) cinsi üzerinde epidermal incelemeler. Yüksek lisans tezi. İnönü Üniversitesi Sağlık Bilimler Enstitüsü.
  • [24] Jin D., Mumper R.J., Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10) (2010)7313-52.
  • [25] Okan O. T., Varlıbaş H., Mehmet Ö.Z., Deniz İ., Antioksidan analiz yöntemleri ve doğu Karadeniz bölgesinde antioksidan kaynağı olarak kullanılabilecek odun dışı bazı bitkisel ürünler. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 13(1) (2013) 48-59.
  • [26] Hossain M.B., Brunton N.P., Patras A., Tiwari B., O’donnell C.P., Martin-Diana A.B., Barry-Ryan C., Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology. Ultrasonics sonochemistry, 19(3) (2012)582-590.
  • [27] Sökmen M., Serkedjieva J., Daferera D., Gulluce M., Polissiou M., Tepe B., Sokmen A., from herbal parts and callus cultures of Origanum acutidens. Journal of agricultural and food chemistry, 52(11)(2004) 3309-3312.
  • [28] Roby M. H. H., Sarhan M. A., Selim K.A.H., Khalel K.I. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Industrial Crops and Products, 43 (2013) 827-831.
  • [29] Celik A., Nur Herken E., Arslan İ., Zafer Özel M.,Mercan N. Screening of the constituents, antimicrobial and antioxidant activity of endemic Origanum hypericifolium O. Schwartz & PH Davis. Natural product research, 24(16) (2010)1568-1577.
  • [30] Fakir H., Us A.A., Sagdic M.,Tornuk F.,Essential Oil Composition, Antimicrobial and Bioactive Properties of Origanuim hypericifolium, An Endemic Plant Species grown in Turkey. Research Journal of Biotechnology,10, 11(2015).
  • [31] Cervato G., Carabelli M., Gervasio S., Cittera A., Cazzola R.,Cestaro B., Antioxbdant properties of oregano (Origanum vulgare) leaf extracts. Journal of Food Biochemistry, 24(6) (2000). 453-465.
  • [32] Özkan G. (2007). Türkiye'de Lamiaceae (Labiatae) familyasına ait baharat veya çeşni olarak kullanılan bazı bitkilerin fenolik bileşenleri ile antioksidan ve antimikrobiyal etkilerinin belirlenmesi (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • [33] Kaurinovic B., Popovic M., Vlaisavljevic S., Trivic S. Antioxidant capacity of Ocimum basilicum L. and Origanum vulgare L. extracts. Molecules, 16(9) (2011) 7401-7414.
  • [34] Baddal M.K. (2010). Origanum Minutiflorum bitkisinin antioksidan özelliklerinin incelenmesi (Doctoral dissertation, SDÜ Fen Bilimleri Enstitüsü).
  • [35] Sarikurkcu C., Zengin G., Oskay M., Uysal S., Ceylan R., Aktumsek A., Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Industrial Crops and Products, 70 (2015)178-184.
  • [36] Yılmaz H., Çarıkçı S., Kılıç T., Dirmenci T., Arabacı T., Gören A.C., Screening of chemical composition, antioxidant and anticholinesterase activity of section Brevifilamentum of Origanum (L.) species. Records of Natural Products, 11(5) (2017).
  • [37] Elhardallou S.B., Cytotoxicity and biological activity of selected Sudanese medicinal plants. Research Journal of Medicinal Plant, 5(3) (2011) 201-29.
  • [38] Taraphdar A.K., Roy M., Bhattacharya R.K. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention, Curr Sci, 80 (2001) 1387-1396.
There are 38 citations in total.

Details

Primary Language English
Subjects Free Radical Chemistry
Journal Section Natural Sciences
Authors

Muhammed Sufi 0009-0006-2626-310X

Ceylan Özsoy 0000-0001-6397-1291

Mehmet Çiçek 0000-0003-4058-3717

Publication Date March 25, 2025
Submission Date September 10, 2024
Acceptance Date January 22, 2025
Published in Issue Year 2025Volume: 46 Issue: 1

Cite

APA Sufi, M., Özsoy, C., & Çiçek, M. (2025). Investigation of Antioxidant Effect of Origanum Hypericifolium Extracts in Different Solvents. Cumhuriyet Science Journal, 46(1), 1-7. https://doi.org/10.17776/csj.1546844
 Download Cover Image