Research Article
BibTex RIS Cite

In Vitro Antioxidant Assessment of Methanol and Water Extracts of Anthemis kotschyana Boiss. var. kotschyana

Year 2024, Volume: 45 Issue: 3, 449 - 453, 30.09.2024

Gülşen Güçlü

https://doi.org/10.17776/csj.1488875

Abstract

The genus Anthemis, with approximately 130 species globally, is significant within the Asteraceae family and is widely used in traditional medicine. Studies on the biological activity of Anthemis kotschyana, an endemic species found in Turkey, are limited. This study investigated the chemical constituents and antioxidant activity of Anthemis kotschyana using two different extracts, methanol and water. The major components of the plant were determined by GC‒MS. According to the data obtained, the major component was found to be "2-propenoic acid, a tridecyl ester" in the water extract and "cyclododecane" in the methanol extract. When the DPPH and ABTS radical scavenging activities were analysed, the methanol extract was found to be more active than the water extract in the DPPH experiment, while the opposite was observed in the ABTS experiment. The TPC was greater than the TFC based on the acquired results. The TPC of the water extract was 83.7 ± 15.6 mg GAE/g, the TPC of the MeOH extract was 170.7 ± 17.4 mg GAE/g, and the TFC of the water extract was 24.3 ± 7.2 QE/g, while the TFC of the MeOH extract was 65.6 ± 5.3 QE/g. For both substances, the amount of methanol extract was greater than that of the water extract. In conclusion, further studies on this species, known for its antioxidant activity and potential as a source of phenolic compounds, could support its use as a therapeutic agent in the future.

Keywords

Anthemis kotschyana Antioxidant DPPH ABTS Medicinal plant.

Thanks

I would like to thank Prof. Dr. Hüseyin Aşkın Akpulat for identifying of the plant.

References

  • [1] Yildiz B., Aktoklu, E., Bitki Sistematiği İlkin Karasal Bitkilerden bir Çeneklilere, Palme Yayıncılık, Ankara, (2010) 1-396.
  • [2] Kadereit J.W., Jeffrey C., (eds), Flowering Plants.Eudicots: Asterales. In Kubitzki K (ed.). The Families and Genera of Vascular Plants, Springer, Verlag Berlin Germany, 8 (2007).
  • [3] Davis P.H., Flora of Turkey and East Aegean Islands, Edinburgh: Edinburgh University Press (1984).
  • [4] Güner A., Özhatay N., Ekim T., Baser, K.H.C., Flora of Turkey and the East Aegean Islands, Edinburgh: Edinburgh University Press, 11 (2000).
  • [5] Güner A., Türkiye Bitkileri Listesi, Nezahat Gökyiğit Botanik Bahçesi yayını, İstanbul, (2013).
  • [6] Jaradat N.A., Ayesh O.I., Anderson C., Ethnopharmacological survey about medicinal plants utilized by herbalists and traditional practitioner healers for treatments of diarrhea in the West Bank/Palestine, Journal of ethnopharmacology, 182 (2016) 57-66.
  • [7] Emir A., Emir C., Anthemis tinctoria L. var. tinctoria L. Bitkisine Ait Fenolik Bileşiklerin LC-ESI-MS/MS ile Miktar Tayini ve Bitkinin Biyolojik Aktivitelerinin Belirlenmesi, Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(4) (2020), 996-1006.
  • [8] Sut S., Dall’Acqua S., Zengin G., Senkardes I., Bulut G., Cvetanović A., Mahomoodally F. Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications, Journal of Pharmaceutical and Biomedical Analysis, 173 (2019) 75–85.
  • [9] Todorova M., Trendafilova A., Danova K., Dimitrov D., Phytochemical study of Anthemis rumelica (Velen) Stoj. & Acth, Biochemical Systematics and Ecology, 39(4–6) (2011) 868–871.
  • [10] Casiglia S., Bruno M., Senatore F., Rosselli S.,Chemical composition of essential oils of Anthemis secundiramea Biv. subsp. secundiramea (Asteraceae) collected wild in Sicily and their activity on microorganisms affecting historical art craft, N Nat Prod Res, 33 (2019) 970-979.
  • [11] Chemsa A.E., Zellagui A., Öztürk M., Erol E., Ceylan O., Duru M.E., Lahouel M., Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of essential oil and methanolic extract of Anthemis stiparum subsp. sabulicola (Pomel) Oberpr, Microb Pathog, 119 (2018) 233-240.
  • [12] Bursal E., Aras A., Kılıç Ö., Buldurun, K., Chemical constituent and radical scavenging antioxidant activity of Anthemis kotschyana Boiss, Natural Product Research, 35(22) (2021) 4794-4797.
  • [13] Güçlü G., İnanır M., Uçar E., Eruygur N., Ataş M., Akpulat H.A., Biological activities of different plant species belonging to the Asteraceae family, International Journal of Secondary Metabolite, 10(1) (2023) 11-22.
  • [14] Tüzün B.S., Fafal T., İlhan R., Kıvçak B., Kırmızıbayrak P.B., Antioxidant, tyrosinase inhibitor, and cytotoxic effects of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsacea (Bornm.) Oberpr. & Greuter, İstanbul Journal of Pharmacy, 53(2) (2023) 193-198.
  • [15] Baroni L., Sarni A.R., Zuliani, C. Plant foods rich in antioxidants and human cognition: A systematic review, Antioxidants, 10(5) (2021) 714.
  • [16] Li A.N., Li S., Li H.B., Xu D.P., Xu X.R., Chen, F., Total phenolic contents and antioxidant capacities of 51 edible and wild flowers, Journal of Functional Foods, 6 (2014) 319– 330.
  • [17] Xu D.P., Li Y., Meng X., Zhou T., Zhou Y., Zheng J., Zhang J.J., Li, H.B., Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources, International Journal of Molecular Sciences, 18(1) (2017) 96.
  • [18] Yu M., Gouvinhas I., Rocha J., Barros A.I., Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources, Scientific Reports, 11(1) (2021).
  • [19] Sacchetti G., Maietti S., Muzzoli M., Scaglianti M., Manfredini S., Radice M., Bruni, R., Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods, Food chemistry, 91(4) (2005) 621-632.
  • [20] Eruygur N., Dural, E., Determination of 1-deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of in-vitro antioxidant, α-amylase and α-glucosidase inhibitory activity in mulberry varieties from Turkey, Phytomedicine, 53 (2019) 234-242.
  • [21] Clarke G., Ting K.N., Wiart C., Fry J., High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest, Antioxidants, 2(1) (2013) 1-10.
  • [22] Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans, C., Antioxidant activity applying an improved ABTS

Year 2024, Volume: 45 Issue: 3, 449 - 453, 30.09.2024

Gülşen Güçlü

https://doi.org/10.17776/csj.1488875

Abstract

References

  • [1] Yildiz B., Aktoklu, E., Bitki Sistematiği İlkin Karasal Bitkilerden bir Çeneklilere, Palme Yayıncılık, Ankara, (2010) 1-396.
  • [2] Kadereit J.W., Jeffrey C., (eds), Flowering Plants.Eudicots: Asterales. In Kubitzki K (ed.). The Families and Genera of Vascular Plants, Springer, Verlag Berlin Germany, 8 (2007).
  • [3] Davis P.H., Flora of Turkey and East Aegean Islands, Edinburgh: Edinburgh University Press (1984).
  • [4] Güner A., Özhatay N., Ekim T., Baser, K.H.C., Flora of Turkey and the East Aegean Islands, Edinburgh: Edinburgh University Press, 11 (2000).
  • [5] Güner A., Türkiye Bitkileri Listesi, Nezahat Gökyiğit Botanik Bahçesi yayını, İstanbul, (2013).
  • [6] Jaradat N.A., Ayesh O.I., Anderson C., Ethnopharmacological survey about medicinal plants utilized by herbalists and traditional practitioner healers for treatments of diarrhea in the West Bank/Palestine, Journal of ethnopharmacology, 182 (2016) 57-66.
  • [7] Emir A., Emir C., Anthemis tinctoria L. var. tinctoria L. Bitkisine Ait Fenolik Bileşiklerin LC-ESI-MS/MS ile Miktar Tayini ve Bitkinin Biyolojik Aktivitelerinin Belirlenmesi, Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(4) (2020), 996-1006.
  • [8] Sut S., Dall’Acqua S., Zengin G., Senkardes I., Bulut G., Cvetanović A., Mahomoodally F. Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications, Journal of Pharmaceutical and Biomedical Analysis, 173 (2019) 75–85.
  • [9] Todorova M., Trendafilova A., Danova K., Dimitrov D., Phytochemical study of Anthemis rumelica (Velen) Stoj. & Acth, Biochemical Systematics and Ecology, 39(4–6) (2011) 868–871.
  • [10] Casiglia S., Bruno M., Senatore F., Rosselli S.,Chemical composition of essential oils of Anthemis secundiramea Biv. subsp. secundiramea (Asteraceae) collected wild in Sicily and their activity on microorganisms affecting historical art craft, N Nat Prod Res, 33 (2019) 970-979.
  • [11] Chemsa A.E., Zellagui A., Öztürk M., Erol E., Ceylan O., Duru M.E., Lahouel M., Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of essential oil and methanolic extract of Anthemis stiparum subsp. sabulicola (Pomel) Oberpr, Microb Pathog, 119 (2018) 233-240.
  • [12] Bursal E., Aras A., Kılıç Ö., Buldurun, K., Chemical constituent and radical scavenging antioxidant activity of Anthemis kotschyana Boiss, Natural Product Research, 35(22) (2021) 4794-4797.
  • [13] Güçlü G., İnanır M., Uçar E., Eruygur N., Ataş M., Akpulat H.A., Biological activities of different plant species belonging to the Asteraceae family, International Journal of Secondary Metabolite, 10(1) (2023) 11-22.
  • [14] Tüzün B.S., Fafal T., İlhan R., Kıvçak B., Kırmızıbayrak P.B., Antioxidant, tyrosinase inhibitor, and cytotoxic effects of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsacea (Bornm.) Oberpr. & Greuter, İstanbul Journal of Pharmacy, 53(2) (2023) 193-198.
  • [15] Baroni L., Sarni A.R., Zuliani, C. Plant foods rich in antioxidants and human cognition: A systematic review, Antioxidants, 10(5) (2021) 714.
  • [16] Li A.N., Li S., Li H.B., Xu D.P., Xu X.R., Chen, F., Total phenolic contents and antioxidant capacities of 51 edible and wild flowers, Journal of Functional Foods, 6 (2014) 319– 330.
  • [17] Xu D.P., Li Y., Meng X., Zhou T., Zhou Y., Zheng J., Zhang J.J., Li, H.B., Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources, International Journal of Molecular Sciences, 18(1) (2017) 96.
  • [18] Yu M., Gouvinhas I., Rocha J., Barros A.I., Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources, Scientific Reports, 11(1) (2021).
  • [19] Sacchetti G., Maietti S., Muzzoli M., Scaglianti M., Manfredini S., Radice M., Bruni, R., Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods, Food chemistry, 91(4) (2005) 621-632.
  • [20] Eruygur N., Dural, E., Determination of 1-deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of in-vitro antioxidant, α-amylase and α-glucosidase inhibitory activity in mulberry varieties from Turkey, Phytomedicine, 53 (2019) 234-242.
  • [21] Clarke G., Ting K.N., Wiart C., Fry J., High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest, Antioxidants, 2(1) (2013) 1-10.
  • [22] Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans, C., Antioxidant activity applying an improved ABTS
There are 22 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry
Journal Section Natural Sciences
Authors

Gülşen Güçlü 0000-0002-3599-213X

Publication Date September 30, 2024
Submission Date May 23, 2024
Acceptance Date August 14, 2024
Published in Issue Year 2024Volume: 45 Issue: 3

Cite

APA Güçlü, G. (2024). In Vitro Antioxidant Assessment of Methanol and Water Extracts of Anthemis kotschyana Boiss. var. kotschyana. Cumhuriyet Science Journal, 45(3), 449-453. https://doi.org/10.17776/csj.1488875
 Download Cover Image