Research Article
Year 2022,
Volume: 43 Issue: 4, 634 - 637, 27.12.2022
Abstract
References
- [1] Cecen B., Ozturk A.B., Yasayan G., Alarcin E., Kocak P., Tutar R., Kozaci L.D., Ryon Shin S., Amir K. Miri, Selection of natural biomaterials for micro‐tissue and organ‐on‐chip models., Journal of Biomedical Materials Research Part A, 110(5) (2022) 1147-1165.
- [2] Menon A., Haritha S., Preethi Soundarya V., Sanjay S., Viji C., Balagangadharan K., Selvamurugan N., Sustained release of chrysin from chitosan-based scaffolds promotes mesenchymal stem cell proliferation and osteoblast differentiation, Carbohydrate Polymers, 195 (2018) 356-367.
- [3] Menon A., Haritha S., Preethi Soundarya V., Sanjay S., Viji C., Balagangadharan K., Selvamurugan N., Sustained release of chrysin from chitosan-based scaffolds promotes mesenchymal stem cell proliferation and osteoblast differentiation, Carbohydrate Polymers, 195 (2018) 356-367.
- [4] Patel S., Shikha S., Manju Rawat S., Deependra S., Preparation and optimization of chitosan-gelatin films for sustained delivery of lupeol for wound healing, International Journal of Biological Macromolecules, 107 (2018) 1888-1897.
- [5] Ranganathan S., Kalimuthu B., Nagarajan S., Chitosan and gelatin-based electrospun fibers for bone tissue engineering, International Journal of Biological Macromolecules, 133 (2019) 354-364.
- [6] Qi L., Zirong X., Minli C., In vitro and in vivo suppression of hepatocellular carcinoma growth by chitosan nanoparticles, European Journal of Cancer, 43(1) (2007) 184-193.
- [7] Chen X., Xiaoming C., He J., Xiangxin C., Xiaoyuan X., Baicheng M., Jie Z., Tao H., SIKVAV-modified chitosan hydrogel as a skin substitutes for wound closure in mice, Molecules, 23(10) (2018) 2611.
- [8] Altuntas S., Harkiranpreet K. Dhaliwal, Nicole J. Bassous, Ahmed E. Radwan, Alpaslan P., Thomas W., Buyukserin F., Mansoor A., Nanopillared Chitosan/Gelatin Films: A Biomimetic Approach for Improved Osteogenesis, ACS Biomaterials Science & Engineering, 5(9) (2019) 4311-4322.
- [9] Li J., and Shaoling Z., Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives, European Polymer Journal 138 (2020) 109984.
- [10] Zhou, N., Na M., Yinchen M., Xiangmin L., Jun Z., Li Li, Jian S., Evaluation of antithrombogenic and antibacterial activities of a graphite oxide/heparin–benzalkonium chloride composite, Carbon, 47(5) (2009) 1343-1350.
- [11] Armstrong J. A., Froelich E. J., Inactivation of viruses by benzalkonium chloride, Applied Microbiology, 12(2) (1964) 132-137.
- [12] Shadman Swarit A., Ishmamul Hoque S., Mohammed Sakib N., Mohidus Samad K., Development of a benzalkonium chloride based antibacterial paper for health and food applications, ChemEngineering, 5(1) (2021) 1.
- [13] Xian M., Liming F., Yili L., Qiang W., Lijing H., Zhonghang Y., Xianyun H., Gang W., Electrical field induce mBMSCs differentiation to osteoblast via protein adsorption enhancement, Colloids and Surfaces B: Biointerfaces, 209 (2022) 112158.
- [14] Kim M., Kyoichi S., Shintaro F., Takanobu S., Jiro O., Water flux and protein adsorption of a hollow fiber modified with hydroxyl groups, Journal of Membrane Science, 56(3) (1991) 289-302.
- [15] Choi Hye-Y., Yong-Hoon L., Cheol-Hong L., Yong-Soon K., In-Seop L., Ji-Min J., Ha-Young L., Hyo-Geun C., Hee Jong W., Dong-Seok S., Assessment of respiratory and systemic toxicity of Benzalkonium chloride following a 14-day inhalation study in rats, Particle and Fibre Toxicology, 17(1) (2020) 1-19.
- [16] Kang Yun M., Sang Hyo L., Ju Young L., Jin Soo S., Byung Soo K., Bong L., Heung Jae C., Byoung Hyun M., Jae Ho K., Moon Suk K., A biodegradable, injectable, gel system based on MPEG-b-(PCL-ran-PLLA) diblock copolymers with an adjustable therapeutic window, Biomaterials, 31(9) (2010) 2453-2460.
- [17] Pereira Rui C., M. Scaranari P., Castagnola M., Grandizio Helena S., Azevedo R. L., Reis Ranieri C., and Chiara G., Novel injectable gel (system) as a vehicle for human articular chondrocytes in cartilage tissue regeneration, Journal of Tissue Engineering and Regenerative Medicine, 3(2) (2009) 97-106.
- [18] Hoque J., Relekar G., Prakash Krishnamoorthy P., Bibek R. S., Jayanta H., Biocompatible injectable hydrogel with potent wound healing and antibacterial properties, Molecular Pharmaceutics, 14(4) (2017) 1218-1230.
- [19] Giano Michael C., Zuhaib I., Scott H.M., Karim A.S., Joani M. C., Yuji Y., Gerald B, Joel P.S., Injectable bioadhesive hydrogels with innate antibacterial properties, Nature Communications, 5(1) (2014) 1-9.
- [20] Saito K., Tohru H., Rihito K., Daijiro M., Kazutaka K., In vitro antibacterial and cytotoxicity assessments of an orthodontic bonding agent containing benzalkonium chloride, The Angle Orthodontist, 79(2) (2009) 331-337
Proliferative and Antimicrobial Evaluation of the Benzalkonium Chloride Loaded Walnut Shell-Rich Chitosan Gels
Abstract
Tissue engineering studies combine cells, biomaterials, and biomolecules to mimic native tissue. The selection of appropriate materials for tissue engineering applications encourages best practices from the lab to clinical trials, and natural biomaterials have the potential to offer desired features for these applications. Material abundance, ease of the process, and biocompatibility are the first milestones to choosing a suitable material. Lignocellulose is one of the most promising biomaterials for its biocompatible, antioxidant, and biodegradable features and is the most abundant material in nature. A walnut shell-added chitosan gel was developed in this study by exploiting chitosan's desired properties, such as biocompatibility, biodegradability, and mechanical capabilities, which boosted cell proliferation. Furthermore, the gel system was reinforced with benzalkonium chloride (BAC), a well-known eye drop sterilizing agent. The hydrogels were subjected to Fourier-transform infrared spectroscopy (FTIR) analyses, and BAC-related signals were observed. The results of BAC-loaded hydrogels revealed that the viability of the primary fibroblasts was enhanced on the BAC-loaded gels compared to tissue culture polystyrene, but the difference was not found statistically significant. Yet, antibacterial activity results demonstrated that only BAC-loaded gel systems have solid antibacterial activity. Additionally, the fibroblasts had the strongest proliferation profile on the walnut shell-added chitosan hydrogels compared to other test groups, but the films' bactericidal activity of the hydrogels was not apparent. After revising the BAC and walnut shell concentrations in the hydrogels, the findings demonstrated that the injectable gel system could be used for cell transplantation in vitro and in vivo.
References
- [1] Cecen B., Ozturk A.B., Yasayan G., Alarcin E., Kocak P., Tutar R., Kozaci L.D., Ryon Shin S., Amir K. Miri, Selection of natural biomaterials for micro‐tissue and organ‐on‐chip models., Journal of Biomedical Materials Research Part A, 110(5) (2022) 1147-1165.
- [2] Menon A., Haritha S., Preethi Soundarya V., Sanjay S., Viji C., Balagangadharan K., Selvamurugan N., Sustained release of chrysin from chitosan-based scaffolds promotes mesenchymal stem cell proliferation and osteoblast differentiation, Carbohydrate Polymers, 195 (2018) 356-367.
- [3] Menon A., Haritha S., Preethi Soundarya V., Sanjay S., Viji C., Balagangadharan K., Selvamurugan N., Sustained release of chrysin from chitosan-based scaffolds promotes mesenchymal stem cell proliferation and osteoblast differentiation, Carbohydrate Polymers, 195 (2018) 356-367.
- [4] Patel S., Shikha S., Manju Rawat S., Deependra S., Preparation and optimization of chitosan-gelatin films for sustained delivery of lupeol for wound healing, International Journal of Biological Macromolecules, 107 (2018) 1888-1897.
- [5] Ranganathan S., Kalimuthu B., Nagarajan S., Chitosan and gelatin-based electrospun fibers for bone tissue engineering, International Journal of Biological Macromolecules, 133 (2019) 354-364.
- [6] Qi L., Zirong X., Minli C., In vitro and in vivo suppression of hepatocellular carcinoma growth by chitosan nanoparticles, European Journal of Cancer, 43(1) (2007) 184-193.
- [7] Chen X., Xiaoming C., He J., Xiangxin C., Xiaoyuan X., Baicheng M., Jie Z., Tao H., SIKVAV-modified chitosan hydrogel as a skin substitutes for wound closure in mice, Molecules, 23(10) (2018) 2611.
- [8] Altuntas S., Harkiranpreet K. Dhaliwal, Nicole J. Bassous, Ahmed E. Radwan, Alpaslan P., Thomas W., Buyukserin F., Mansoor A., Nanopillared Chitosan/Gelatin Films: A Biomimetic Approach for Improved Osteogenesis, ACS Biomaterials Science & Engineering, 5(9) (2019) 4311-4322.
- [9] Li J., and Shaoling Z., Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives, European Polymer Journal 138 (2020) 109984.
- [10] Zhou, N., Na M., Yinchen M., Xiangmin L., Jun Z., Li Li, Jian S., Evaluation of antithrombogenic and antibacterial activities of a graphite oxide/heparin–benzalkonium chloride composite, Carbon, 47(5) (2009) 1343-1350.
- [11] Armstrong J. A., Froelich E. J., Inactivation of viruses by benzalkonium chloride, Applied Microbiology, 12(2) (1964) 132-137.
- [12] Shadman Swarit A., Ishmamul Hoque S., Mohammed Sakib N., Mohidus Samad K., Development of a benzalkonium chloride based antibacterial paper for health and food applications, ChemEngineering, 5(1) (2021) 1.
- [13] Xian M., Liming F., Yili L., Qiang W., Lijing H., Zhonghang Y., Xianyun H., Gang W., Electrical field induce mBMSCs differentiation to osteoblast via protein adsorption enhancement, Colloids and Surfaces B: Biointerfaces, 209 (2022) 112158.
- [14] Kim M., Kyoichi S., Shintaro F., Takanobu S., Jiro O., Water flux and protein adsorption of a hollow fiber modified with hydroxyl groups, Journal of Membrane Science, 56(3) (1991) 289-302.
- [15] Choi Hye-Y., Yong-Hoon L., Cheol-Hong L., Yong-Soon K., In-Seop L., Ji-Min J., Ha-Young L., Hyo-Geun C., Hee Jong W., Dong-Seok S., Assessment of respiratory and systemic toxicity of Benzalkonium chloride following a 14-day inhalation study in rats, Particle and Fibre Toxicology, 17(1) (2020) 1-19.
- [16] Kang Yun M., Sang Hyo L., Ju Young L., Jin Soo S., Byung Soo K., Bong L., Heung Jae C., Byoung Hyun M., Jae Ho K., Moon Suk K., A biodegradable, injectable, gel system based on MPEG-b-(PCL-ran-PLLA) diblock copolymers with an adjustable therapeutic window, Biomaterials, 31(9) (2010) 2453-2460.
- [17] Pereira Rui C., M. Scaranari P., Castagnola M., Grandizio Helena S., Azevedo R. L., Reis Ranieri C., and Chiara G., Novel injectable gel (system) as a vehicle for human articular chondrocytes in cartilage tissue regeneration, Journal of Tissue Engineering and Regenerative Medicine, 3(2) (2009) 97-106.
- [18] Hoque J., Relekar G., Prakash Krishnamoorthy P., Bibek R. S., Jayanta H., Biocompatible injectable hydrogel with potent wound healing and antibacterial properties, Molecular Pharmaceutics, 14(4) (2017) 1218-1230.
- [19] Giano Michael C., Zuhaib I., Scott H.M., Karim A.S., Joani M. C., Yuji Y., Gerald B, Joel P.S., Injectable bioadhesive hydrogels with innate antibacterial properties, Nature Communications, 5(1) (2014) 1-9.
- [20] Saito K., Tohru H., Rihito K., Daijiro M., Kazutaka K., In vitro antibacterial and cytotoxicity assessments of an orthodontic bonding agent containing benzalkonium chloride, The Angle Orthodontist, 79(2) (2009) 331-337
There are 20 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Biomaterial |
| Journal Section | Natural Sciences |
| Authors | |
| Publication Date | December 27, 2022 |
| Submission Date | May 29, 2022 |
| Acceptance Date | October 12, 2022 |
| Published in Issue | Year 2022Volume: 43 Issue: 4 |
