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Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells

Year 2022, Volume 43, Issue 3, 365 - 369, 30.09.2022
https://doi.org/10.17776/csj.1108095

Abstract

Glial tumors are the largest and danger group of central nervous system tumors. The use of natural products now has been contemplated of exceptional value in the control of cancer. Mushrooms have been used for many centuries, not just as a food, but also to treat many illnesses. Therefore, we aimed to evaluate the effect of exopolysaccharides (EPS) obtained from six different edible mushrooms on the survival of glioma cells. In this study the effects of 0.4, 1, 2, 4 and 6 μg/mL doses of EPSs from six mushroom species Coprinus comatus, Fistulina hepatica, Panus neostrigosus, Laetiporus sulphureus, Polyporus squamosus, and Lenzites betulinus were investigated on the rat glioma cell line (C6) in two different periods by MTT assay. According to our results 0.4 and 1 μg/mL of EPSs from six mushroom species were not effective or less effective, but 2, 4 and 6 μg/mL doses killed glioma cells about 27 to 71 % for 24 hours, 35 to 78 % for 48 hours As a result, these mushroom EPSs showed different cytotoxicity to glioma cells time and dose-dependently. These findings can be suggested that the anti-tumor effects of EPSs can be potential use in clinical applications to treat glioma. Further studies are needed to understand these effects more clearly on glioma.

References

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  • [23] Kidd P.M., The use of mushroom glucans and proteoglycans in cancer treatment, Alternative Medicine Review, 5 (2000) 4–27.
  • [24] Borchers A., Keen C.L., Gershwin M.E., Mushrooms, tumors, and immunity: an Update, Experimental Biology and Medicine, 229 (2004) 393–406.
  • [25] Ren L., Perera C., Hemar Y., Antitumor activity of mushroom polysaccharides: a Review, Food and Function, 3 (2012) 1118–1130.
  • [26] Zaidman B.Z., Wasser S.P., Nevo E., Mahajna J., Coprinus comatus and Ganodermalucidum interfere with androgen receptor function in LNCaP prostate cancer cell, Molecular Biology Reports, 35 (2008) 107–117.
  • [27] Wong J.H., Ng T.B., Chan H.H.L., Liu Q., Man G.C.W., Zhang C.Z., Guan S., Ng C.C.W., Fang E.F., Wang H., Liu F., Ye X., Rolka K., Naude R., Zhao S., Sha O., Li C., Xia L., Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials, Applied Microbiology and Biotechnology, 104 (2020) 4675–4703.
  • [28] Zekovic D.B., Kwiatkowski S., Vrvic M.M., Jakovljevic D., Moran C.A., Natural modified (1-3)-β-glucans in health promotion and disease alleviation, Critical Reviews in Biotechnology, 25 (2005) 205–230.

Year 2022, Volume 43, Issue 3, 365 - 369, 30.09.2022
https://doi.org/10.17776/csj.1108095

Abstract

References

  • [1] Barillot E., Calzone L., Hupe P., Vert J.P., Zinovyev A., Computation system biology of cancer, USA: CRC Press, Taylor and Francis Group. A Chapman and Hall Book, (2013).
  • [2] Jemal A., Siegel R., Ward E., Hao Y., Xu J., Thun M.J., Cancer statistics CA, A Cancer Journal for Clinicians, 59(4) (2009) 225-49.
  • [3] Zjawiony J.K., Biologically active compounds from Aphyllophorales (Polypore) Fungi, Journal of Natural Products, 67(2) (2004) 300-310.
  • [4] Wasser S.P., Medicinal mushroom science: Current Perspectives, Advances, Evidences, and Challenges, Biomedical Journal, 37(6) (2014) 345-356.
  • [5] Savelkoul H.F.J., Chanput W., Wichers H.J., Immunomodulatory effects of mushroom β-glucans. In: Diet, Immunity and Inflammation, (Eds. PC Calder and P Yaqoob): 416–434. Woodhead Publishing Series in Food Science, Technology and Nutrition, (2013).
  • [6] Sanchez C., Bioactives from Mushroom and Their Application. In: Food Bioactives, (Ed M Puri): 23–57. Springer International Publishing, (2007).
  • [7] Khan A.A., Gani A., Khanday F.A., Masoodi F.A., Biological and pharmaceutical activities of mushroom β-glucan discussed as a potential functional food ingredient, Bioactive Carbohydrates and Dietary Fibre, 16 (2018) 1–13.
  • [8] Chaturvedi V.K., Agarwal S., Gupta K.K., Ramteke P.W., Singh M.P., Medicinal mushroom: boon for therapeutic applications, 3 Biotech, 8 (2018) 334.
  • [9] Wasser S.P., Medicinal mushrooms as a source of antitumor and immunomodulation Polysaccharides, Applied Microbiology and Biotechnology, 60 (2002) 258–274.
  • [10] Wasser S.P., Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: A review, International Journal of Medicinal Mushrooms, 19 (2017) 279–317.
  • [11] Vitak T., Yurkiv B., Wasser S., Nevo E., Sybirna N., Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus, World Journal of Diabetes, 8(5) (2017) 187–201.
  • [12] Dudekula U.T., Doriya K., Devarai S.K., A critical review on submerged production of mushroom and their bioactive metabolites, 3 Biotech, 10 (2020) 337.
  • [13] Nowakowski P., Markiewicz Z.R., Gromkowska-Kępka K, Naliwajko S.K., Moskwa J., Bielecka J., Grabia M., Borawska M., Socha K., Mushrooms as potential therapeutic agents in the treatment of cancer: Evaluation of anti-glioma effects of Coprinus comatus, Cantharellus cibarius, Lycoperdon perlatum and Lactarius deliciosus extracts, Biomedicine & Pharmacotherapy, 133 (2021) 111094.
  • [14] Zhang M., Cui S.W., Cheung P.C.K., Wang Q., Antitumor polysaccharides from mushrooms: a review on their isolation process structural characteristics and antitumor activity, Trends in Food Science and Technology, 18 (2007) 4–19.
  • [15] Lucas E.H., Tumor inhibition in Boletus edulis and other Holobasidiomycetes, Antibiotic Chemotherapy, 7 (1957) 1-15.
  • [16] Lindequist U., Niedermeyer T.H.J., Ju¨ lich W.D., The pharmacological potential of Mushrooms, Evidence-Based Complementary and Alternative Medicine, 2 (2005) 285–299.
  • [17] Deng C., Hu Z., Fu H.T., Hu M.H., Xu X., Chen J.H., Chemical analysis and antioxidant activity in vitro of a β-D-glucan isolated from Dictyophora indusiate, International Journal of Biological Macromolecules, 51 (2012) 70–75.
  • [18] Minari M.C., Rincao V.P., Soares S.A., Ricardo N.M., Nozawa C., Linhares R.E., Antiviral properties of polysaccharides from Agaricus brasiliensis in the replication of bovine herpesvirus 1, Acta Virologica, 55 (2011) 255–259.
  • [19] Wasser S.P., Weis A.L., Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current perspectives (review), International Journal of Medicinal Mushrooms, 1 (1999) 31–62.
  • [20] Yamaç M, Kanbak G, Zeytinoğlu M, Bayramoğlu G, Şentürk H, Uyanoğlu M. Hypoglycemic effect of Lentinus strigosus (schwein.) Fr. crude exopolysaccharide in streptozotocin induced diabetic rats, Journal of Medicinal Food, 11(3) (2008) 513–517.
  • [21] Uyanoglu M., Yamac M., Canbek M., Sentürk, H., Kartkaya K., Oglakçı A., Turgak O.,Kanbak G., Curative effect of crude exopolysaccharides of some macrofungi on alcohol-induced liver damage, Ultrastructural Pathology, 37(3) (2013) 218–226.
  • [22] Pillai T.G., Devi U.P., Mushroom beta glucan: potential candidate for post irradiation Protection, Mutation Research, 751 (2013) 109–115.
  • [23] Kidd P.M., The use of mushroom glucans and proteoglycans in cancer treatment, Alternative Medicine Review, 5 (2000) 4–27.
  • [24] Borchers A., Keen C.L., Gershwin M.E., Mushrooms, tumors, and immunity: an Update, Experimental Biology and Medicine, 229 (2004) 393–406.
  • [25] Ren L., Perera C., Hemar Y., Antitumor activity of mushroom polysaccharides: a Review, Food and Function, 3 (2012) 1118–1130.
  • [26] Zaidman B.Z., Wasser S.P., Nevo E., Mahajna J., Coprinus comatus and Ganodermalucidum interfere with androgen receptor function in LNCaP prostate cancer cell, Molecular Biology Reports, 35 (2008) 107–117.
  • [27] Wong J.H., Ng T.B., Chan H.H.L., Liu Q., Man G.C.W., Zhang C.Z., Guan S., Ng C.C.W., Fang E.F., Wang H., Liu F., Ye X., Rolka K., Naude R., Zhao S., Sha O., Li C., Xia L., Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials, Applied Microbiology and Biotechnology, 104 (2020) 4675–4703.
  • [28] Zekovic D.B., Kwiatkowski S., Vrvic M.M., Jakovljevic D., Moran C.A., Natural modified (1-3)-β-glucans in health promotion and disease alleviation, Critical Reviews in Biotechnology, 25 (2005) 205–230.

Details

Primary Language English
Subjects Biology
Journal Section Natural Sciences
Authors

Serife CAN CAKA>
Dr. Burhan Nalbantoglu Devlet Hastanesi
0000-0003-2265-9124
Türkiye


Pınar ÖZTOPCU VATAN>
ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ
0000-0003-4339-4129
Türkiye


Selda KABADERE> (Primary Author)
ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ
0000-0002-9589-0063
Türkiye


Mustafa YAMAÇ>
ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ
0000-0002-7262-0036
Türkiye


Ruhi UYAR>
It is not affiliated with an institution
0000-0002-7608-7531
Türkiye

Publication Date September 30, 2022
Application Date April 23, 2022
Acceptance Date August 27, 2022
Published in Issue Year 2022, Volume 43, Issue 3

Cite

APA Can Caka, S. , Öztopcu Vatan, P. , Kabadere, S. , Yamaç, M. & Uyar, R. (2022). Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells . Cumhuriyet Science Journal , 43 (3) , 365-369 . DOI: 10.17776/csj.1108095