Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity

Dilara Ülger Özbek; Deniz Kuruçay

doi:10.17776/csj.1088724

EN

Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity

Abstract

Aim: Aluminum is one of the elements that is widely used in many sectors and is the most abundant element in nature. The harm of aluminum, which was thought to be harmless until recently and is actively used in daily life, is open to discussion. In this study, it was aimed to investigate the effect of Aluminum Sulphate [Al2(SO4)3] on Glucose-6-Phosphate Dehydrogenase (G6PDH) activity, which is a key enzyme that catalyzes the first step of the pentose phosphate pathway (PFP). In addition, enzyme activity are detailed with molecular docking studies. For the purpose of examining in vitro effect of Aluminium on G6PDH, 4 different concentration of substrate (D-glucose-6-P) (01.M, 0.08M, 0.05M, 0.03M) prepared and 10mM, 30mM Al2(SO4)3 was added G6PDH envoriment. G6PDH activity was measured by spectrofotometrically. Molecular docking studies were performed with DockingServer and HEX 8.0.0 programs. With the data obtained, the Vmax of G6PDH was calculated as 3.33 and Km=0.0323. When 10 mM and 30mM Al2(SO4)3 were added to the reaction environment, it was observed that there was a decrease in enzyme activity by 24.92% and 57.06%, respectively. It was observed that the increase in Al2(SO4)3 concentration was an uncompetitive inhibition due to a significant decrease in both Km and Vmax values of the enzyme.

Keywords

References

[1] Yılmaz E., Farklı Dozlardaki Alüminyum Klorür’ün Galleria Mellonella (lepıdoptera: pyralıdae)’nın Biyolojisine ve Hemosit Sayılarına Etkileri (Doctoral dissertation), Marmara Üniversitesi, (2013).
[2] Ak Uysal H., Ergene N.B., Alüminyum ve İnsan Sağlığı, SÜ Tıp Fakültesi Dergisi, 6(2) (1990) 230-237.
[3] Yenigül E., Alüminyum ve Bazı Organik Asitlerin Etkileşiminin Spektrometrik ve Kromatografik Yöntemlerle Tayini, (2006).
[4] Alfrey A.C., Aluminum, Advances in Clinical Chemistry, 23 (1983) 69-91.
[5] Ojeda C. B., Rojas F.S., Pavón J.C., Torres A.G., Automated Online Separation Preconcentration System For Platinum Determination by Electrothermal Atomic Absorption Spectrometry, Analytica Chimica Acta, 494 (1-2) (2003) 97-103.
[6] Akpolat T., Utaş C., Hemodiyaliz Hekimi El Kitabı, Kayseri, Anadolu Yayıncılık (2001).
[7] Becaria A., Campbell A., Bondy S. C., Aluminum as a Toxicant, Toxicology and Industrial Health, 18(7) (2002) 309-320.
[8] Haberhauer G., Ja A.A., Tunega D., Gerzabek Martin H., Lischka H., Potential of Theoretical Calculations to Estimate the Stability of Al3+/Citrate, Oxalate and Acetate Complexes, In 17. World congress of Soil Science, Bangkok (Thailand), August (2002).

[9] Savory J., Opening of the Mitochondrial Permeability Transition Pore by Reactive Oxygen Species is a Basic Event Neurodegeneration, Virginia Univ Charlottesville, (2001).
[10] Telefoncu A., Telefoncu F., Glukoz-6-fosfat Dehidrogenaz Aktivitesine Primaquine’nin Etkisi, Tr. J. Medical Sci., 14 (1989) 57-63.
[11] Greer J. P., Arber D.A., Glader B., List A.F., Means R.T., Paraskevas F., Rodgers G.M., Wintrobe's Clinical Hematology, Lippincott Williams & Wilkins (2013).
[12] Sodeman W. A., TM., Sodeman’s Pathologic Physiology Mechanisms of Disease, 1 (1992).
[13] Kaya S., Erkan S., Karakaş D., Computational Investigation of Molecular Structures, Spectroscopic Properties and Antitumor-Antibacterial Activities of Some Schiff Bases. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 244 (2021) 118829.
[14] Von Worthington, Worthington Enzyme Manual; Enymes And Related Biochemicals, 1.1.1.49 Glucose-6-Phosphate Dehydrogenase, (1993) 189-190.
[15] Bikadi Z., Hazai E., Application of the PM6 Semi-empirical Method to Modeling Proteins Enhances Docking Accuracy of AutoDock, Journal of Cheminformatics, 1(1) (2009) 1-16.
[16] Ritchie D., Orpailleur T., Hex 8.0. 0 User Manual. Protein Docking Using Spherical Polar Fourier Correlations Copyright c (2013).
[17] Rowland P., Basak A.K., Gover S., Levy H.R., Adams M.J., The Three Dimensional Structure of Glucose 6–Phosphate Dehydrogenase from Leuconostoc Mesenteroides Refined At 2.0 Å Resolution, Structure,, 2(11) (1994) 1073-1087.
[18] Erkan S., Activity of the Rocuronium Molecule and its Derivatives: A Theoretical Calculation, Journal of Molecular Structure, 1189 (2019) 257-264.
[19] Erkan S., Structural, Spectroscopic and Anti-Cancer Properties of Hydroxy-and Sulfonamide-Azobenzene Platinum (II) Complexes: DFT and Molecular Docking Studies, Cumhuriyet Science Journal, 39(4) (2018) 1036-1051.
[20] Erkan S., Kaya S., Sayin K., Karakaş D., Structural, Spectral Characterization and Molecular Docking Analyses of Mer-Ruthenium (II) Complexes Containing the Bidentate Chelating Ligands, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 224 (2020) 117399.
[21] Merugu R., Neerudu U.K., Dasa K., Singh K.V., Molecular Docking Studies of Deacetylbisacodyl with Intestinal Sucrase-Maltase Enzyme, Int. J. Adv. Sci. Res., 2(12) (2016) 191-193.
[22] Bosman G., Willekens F., Werre J., Erythrocyte Aging: a More Than Superficial Resemblance to Apoptosis, Cellular Physiology and Biochemistry, 16(1-3) (2005) 1-8.
[23] Lang K. S., Duranton C., Poehlmann H., Myssina S., Bauer C., Lang F., Huber S.M., Cation Channels Trigger Apoptotic Death of Erythrocytes, Cell Death & Differentiation, 10(2) (2003) 249-256.
[24] Duranton C., Huber S. M., Lang F., Oxidation Induces a Cl− Dependent Cation Conductance in Human Red Blood Cells, The Journal of Physiology, 539(3) (2002) 847-855.
[25] Matarrese P., Straface E., Pietraforte D., Gambardella L., Vona R., Maccaglia A., Malorni W., Peroxynitrite Induces Senescence and Apoptosis of Red Blood Cells Through the Activation of Aspartyl and Cysteinyl Proteases, The FASEB Journal, 19(3) (2005) 1-27.
[26] Fico A., Paglialunga F., Cigliano L., Abrescia P., Verde P., Martini G., Filosa S., Glucose-6-Phosphate Dehydrogenase Plays a Crucial Role in Protection From Redox-Stress-İnduced Apoptosis, Cell Death & Differentiation, 11(8) (2004) 823-831.
[27] Spitz D.R., Azzam E.I., Jian Li J., Gius D., Metabolic Oxidation/Reduction Reactions and Cellular Responses to Ionizing Radiation: a Unifying Concept in Stress Response Biology, Cancer and Metastasis Reviews, 23(3) (2004) 311-322.
[28] Fairbanks V.F., Copper Sulfate-induced Hemolytic Anemia: İnhibition of Glucose-6-Phosphate Dehydrogenase and Other Possible Etiologic Mechanisms, Archives of İnternal Medicine, 120(4) (1967) 428-432.
[29] Danielyan K. E., Babayan L. A., Chailyan S. G., Impact of the Heavy Metals on the Activity of Xanthine Oxidoreduc-tase, Appl Clin Pharmacol Toxicol, 3(123) (2019) 2577-0225.
[30] Budak H., Ceylan H., Kocpinar E.F., Gonul N., Erdogan O., Expression of Glucose‐6‐Phosphate Dehydrogenase and 6‐Phosphogluconate Dehydrogenase in Oxidative Stress Induced by Long‐Term Iron Toxicity in Rat Liver, Journal of Biochemical and Molecular Toxicology, 28(5) (2014) 217-223.
[31] Exley C., Korchazhkina O.V., Promotion of formation of Amyloid Fibrils By Aluminium Adenosine Triphosphate (AlATP), Journal of Inorganic Biochemistry, 84(3-4) (2001) 215-224.
[32] Merian E., Anke M., Ihnat M., Stoeppler M., Elements and Their Compounds in the Environment, Occurrence, Analysis and Biological Relevance 2nd ed. Wiley-VCH Verlag GmbH and Co. KGaA (2004). (https://www.cabdirect.org/cabdirect/abstract/20043118056).
[33] Serencam H., Arslaner A., Müge K., Civil Peynirde Ağır Metal Kontaminasyon Kaynağı ve Düzeyinin Belirlenmesi Üzerine Bir Araştırma, Engineering Sciences, 13(1) (2018) 21-26.
[34] Onur E. Alüminyum Toksisitesinin Kalite Kontrol Açısından Değerlendirilmesi, Türk Nefroloji Diyaliz ve Transplantasyon Dergisi, 1(2) (1997).
[35] Recker R.R., Blotcky A.J., Leffler J.A., Rack, E.P., Evidence of Aluminum Absorption from the Gastrointestinal Tract and Bone Deposition by Aluminum Carbonate Ingestion with Normal Renal Function, The Journal of Laboratory and Clinical Medicine, 90(5) (1977) 810-815.
[36] Clapp T., Siebert P., Chen D., Braun L.J., Vaccines with Aluminum-Containing Adjuvants: Optimizing Vaccine Efficacy and Thermal Stability, Journal of Pharmaceutical Sciences, 100(2) (2011) 388-401.
[37] Klaassen C.D. (Ed.), Casarett and Doull's Toxicology: The Basic Science of Poisons. New York: McGraw-Hill, 1236 (2013) 189.
[38] Bakar C., Baba A., Metaller ve İnsan Sağlığı: Yirminci Yüzyıldan Bugüne ve Geleceğe Miras Kalan Çevre Sağlığı Sorunu, 1. Tıbbi Jeoloji Çalıştayı, (2009).
[39] Kutlubay R., Oğuz E.O., Güven C., Can B., Sinik Z., Tuncay Ö.L., Histological and Ultrastructural Evidence for Protective Effects on Aluminium-induced Kidney Damage by Intraperitoneal Administration of α-Tocopherol, International Journal of Toxicology, 26(2) (2007) 95-101.
[40] Oğuz E. O., Yüksel H., Havane E., Yaşar E., Zorbozan O., Zuhal C., Turgut G., Aluminyum Sülfat’ın “Ross” Cinsi Term Besi Civcivi Karaciğerinde Yarattığı Toksik Ve Inflamatuar Hasar, Ankara Üniversitesi Tıp Fakültesi Mecmuası, 61(1) (2008) 1-6.
[41] Pai S., Melethil S., Cuddy P., Hall T., Elevation of Serum Aluminum in Humans on a two‐day Sucralfate Regimen, The Journal of Clinical Pharmacology, 27(3) (1987) 213-215.
[42] Günaydin N., Uzun Süreli Alüminyum Kaplarda Yapılan Yoğurtlarla Beslenenlerde Plazma Alüminyum Seviyeleri ile Oksidatif Durum Arasındaki İlişkinin Araştırılması (Master thesis), Sağlık Bilimleri Enstitüsü, (2005).
[43] Fridovich I., The Biology of Oxygen Radicals: The Superoxide Radical is an Agent of Oxygen Toxicity; Superoxide Dismutases Provide an Important Defense, Science, 201(4359) (1978) 875-880.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Dilara Ülger Özbek ^*
0000-0002-6834-020X
Türkiye

Deniz Kuruçay
0000-0002-9255-3301
Türkiye

Publication Date

June 29, 2022

Submission Date

March 17, 2022

Acceptance Date

May 28, 2022

Published in Issue

Year 1970 Volume: 43 Number: 2

DOI

https://doi.org/10.17776/csj.1088724

IZ

https://izlik.org/JA27BW84WZ

Cite

RIS / Bibtex

APA

Ülger Özbek, D., & Kuruçay, D. (2022). Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. Cumhuriyet Science Journal, 43(2), 232-237. https://doi.org/10.17776/csj.1088724