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Year 2020, Volume: 41 Issue: 4, 815 - 825, 29.12.2020

Ozan Yağmuroğlu , Sibel Emir Diltemiz

https://doi.org/10.17776/csj.725122

Abstract

References

  • [1] Yağmuroğlu O., Development of paraoxon based sensors for determination of chemical warfare agents, Anadolu University, Doctoral thesis (2017).
  • [2] Marrs T.C., Balantyne B., Pesticide toxicology. New York: Wiley, 2002; 80-120.
  • [3] Sunay S., Investigation of paraoxonase polymorphism and paraoxonase enzyme activity in individuals exposed to pesticides, Ankara University, Doctoral thesis, (2010).
  • [4] Jeyeratnam J., Pesticides: poisining as a global health problem, World Health Stat., Q.43 (1990) 139-144.
  • [5] Öğüt S., Determination of the effects of some pesticides used in Isparta region on the pesticide residues in apple-cherry and the blood parameters of agricultural workers working in the agriculture of these products, Süleyman Demirel University, Doctoral thesis (2012).
  • [6] Gürcan T., Pesticide residues and their importance, Journal of World Food, May Number (2001) 67-72.
  • [7] Kaya, S. Pesticides and the main problems they can cause. Ankara University, Veterinary Congress, Ankara, (1996).
  • [8] Şanlı, Y., Environmental problems and food contamination, Veterinary Journal of Selçuk University, Special Issue (1984) 17-37.
  • [9] Kamanyire R., Karalliedde L., Organophosphate toxicity and occupational exposure, Occup Med (Lond), 54 (2004) 69–75.
  • [10] Hıncal F., Çeliker A., Özgüven Ş., Kaya E., Effects of chemical and biological warfare agents on health, Ankara: Hacettepe University, 1991; 20-50.
  • [11] Karayılanoğlu T., Medical defenses and pesticides in chemical attack, Ankara: GATA, 2003; 100-130.
  • [12] Yağmuroğlu O., Chemical defense and security, Eskişehir: Anadolu University, 2018; 3-30.
  • [13] Yağmuroğlu O., Mini Review Adsorption and Decomposition of Chemical Warfare Agents by Metal-Organic Framework, Biomedical Journal of Scientific & Technical Research, 26(1) (2020) 19691-19694.
  • [14] Patocka J., Kuča K., Jun D., Acetylcholinesterase and butyrylcholinesterase – Important enzymes of human body, Acta Medica (Hradec Králové), 47 (2004) No. 4.
  • [15] Patocka J., Kuca K., Jun D., Cabal J., Oxime reactivation of acetylcholinesterase inhibited by toxic phosphorus esters: In vitro kinetics and thermodynamics, J.Appl.Biomed., 3 (2005) 91-99.
  • [16] Bajgar J., Organophosphates/Nevre Agents poisoning: mechanism of action, diagnosis, prophylaxis and treatment, Advances In Clinical Chemistry, 38 (2004) 152-153.
  • [17] Diltemiz S., Yağmuroğlu O., Development reflectometric interference spectroscopy based sensor for paraoxon determination, Eskişehir Technical University Journal of Science and Technology C - Life Sciences and Biotechnology, 8 (1) (2019) 12-22.
  • [18] Wang J., Krause R., Block K., Musameh M., Mulchandani A., Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chemica Acta, 469 (2009) 197-203.
  • [19] Hossain M., Faisal M., Kim C., Cha H., Nam S., Lee J., Amperometric proton selective strip-sensors with a microelliptic liquid/gel interface for organophosphate neurotoxins, Electrochemistry Communication, 13 (2011) 611-614.
  • [20] Lei C., Valenta M., Saripalli P., Ackerman J., Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica, J. Environ. Qual., 36 (2007) 233-238.
  • [21] Meng Z., Yamazaki T., Sode K., Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular impirinting technique, Biotechnol. Lett., 25 (2003) 1075-1080.
  • [22] Wang J., Krause R., Block K., Musameh M., Mulchandani A., Mulchandani P., Schöning M.J., Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chimica Acta, 469(2) (2002) 197–203.
  • [23] Dragonov D.I., Ladu B.N., Pharmacogenetics of paraoxonases: a brief review, Naunyn-Schmiedeberg’s Arch Pharmocol, 369(1) (2004) 78-88.
  • [24] Yağmuroğlu O., Diltemiz S.E., Development of QCM based biosensor for the selective and sensitive detection of paraoxon, Analytical Biochemistry, 591 (2020) 113572.

Development of acetylcholinesterase immobilized CMD (Carboxymethyldextran) chip-based sensor for the detection of nerve agent simulant parathion

Year 2020, Volume: 41 Issue: 4, 815 - 825, 29.12.2020

Ozan Yağmuroğlu , Sibel Emir Diltemiz

https://doi.org/10.17776/csj.725122

Abstract

In this study, a carboxymethyldextran chip based sensor system is developed that selectively recognizes and binds nerve agent molecules used in chemical weapons. Nerve agents fall under the group of organophosphorus compounds and irreversibly inhibit the acetylcholinesterase enzyme (AChE). In this study, parathion was used as an organophosphorus compound. The effect of the parathion molecule on enzyme inhibition is similar to nerve agents. The first step to be applied before CMD (Carboxymethyldextran) chip surface enzyme immobilization is the surface activation. After the surface activation was completed, AChE enzyme solution was passed over the chip surface for 40 minutes. In this way, enzyme immobilization was performed on the chip surface and a surface selective to the parathion molecule was obtained. Analysis was performed for parathion samples in different concentrations in the range of 3.43x10-8-6.86x10-4 mol/L. When the analysis results were transferred to the calibration graph, a graphic close to the linear was obtained. The working range of the chip surface developed as a result of the analyzes was calculated as 3.43x10-8 - 6.86x10-4, the limit of detection (LOD) value was 3.79x10-8 and the limit of quantification (LOQ) value was 6.16x10-8. These results show that samples containing parathion at very low concentrations can be analyzed using the method we have developed. 

Keywords

Nerve agents Organophosphorus compounds Sensors Reflactometric interference spectroscopy Parathion

References

  • [1] Yağmuroğlu O., Development of paraoxon based sensors for determination of chemical warfare agents, Anadolu University, Doctoral thesis (2017).
  • [2] Marrs T.C., Balantyne B., Pesticide toxicology. New York: Wiley, 2002; 80-120.
  • [3] Sunay S., Investigation of paraoxonase polymorphism and paraoxonase enzyme activity in individuals exposed to pesticides, Ankara University, Doctoral thesis, (2010).
  • [4] Jeyeratnam J., Pesticides: poisining as a global health problem, World Health Stat., Q.43 (1990) 139-144.
  • [5] Öğüt S., Determination of the effects of some pesticides used in Isparta region on the pesticide residues in apple-cherry and the blood parameters of agricultural workers working in the agriculture of these products, Süleyman Demirel University, Doctoral thesis (2012).
  • [6] Gürcan T., Pesticide residues and their importance, Journal of World Food, May Number (2001) 67-72.
  • [7] Kaya, S. Pesticides and the main problems they can cause. Ankara University, Veterinary Congress, Ankara, (1996).
  • [8] Şanlı, Y., Environmental problems and food contamination, Veterinary Journal of Selçuk University, Special Issue (1984) 17-37.
  • [9] Kamanyire R., Karalliedde L., Organophosphate toxicity and occupational exposure, Occup Med (Lond), 54 (2004) 69–75.
  • [10] Hıncal F., Çeliker A., Özgüven Ş., Kaya E., Effects of chemical and biological warfare agents on health, Ankara: Hacettepe University, 1991; 20-50.
  • [11] Karayılanoğlu T., Medical defenses and pesticides in chemical attack, Ankara: GATA, 2003; 100-130.
  • [12] Yağmuroğlu O., Chemical defense and security, Eskişehir: Anadolu University, 2018; 3-30.
  • [13] Yağmuroğlu O., Mini Review Adsorption and Decomposition of Chemical Warfare Agents by Metal-Organic Framework, Biomedical Journal of Scientific & Technical Research, 26(1) (2020) 19691-19694.
  • [14] Patocka J., Kuča K., Jun D., Acetylcholinesterase and butyrylcholinesterase – Important enzymes of human body, Acta Medica (Hradec Králové), 47 (2004) No. 4.
  • [15] Patocka J., Kuca K., Jun D., Cabal J., Oxime reactivation of acetylcholinesterase inhibited by toxic phosphorus esters: In vitro kinetics and thermodynamics, J.Appl.Biomed., 3 (2005) 91-99.
  • [16] Bajgar J., Organophosphates/Nevre Agents poisoning: mechanism of action, diagnosis, prophylaxis and treatment, Advances In Clinical Chemistry, 38 (2004) 152-153.
  • [17] Diltemiz S., Yağmuroğlu O., Development reflectometric interference spectroscopy based sensor for paraoxon determination, Eskişehir Technical University Journal of Science and Technology C - Life Sciences and Biotechnology, 8 (1) (2019) 12-22.
  • [18] Wang J., Krause R., Block K., Musameh M., Mulchandani A., Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chemica Acta, 469 (2009) 197-203.
  • [19] Hossain M., Faisal M., Kim C., Cha H., Nam S., Lee J., Amperometric proton selective strip-sensors with a microelliptic liquid/gel interface for organophosphate neurotoxins, Electrochemistry Communication, 13 (2011) 611-614.
  • [20] Lei C., Valenta M., Saripalli P., Ackerman J., Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica, J. Environ. Qual., 36 (2007) 233-238.
  • [21] Meng Z., Yamazaki T., Sode K., Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular impirinting technique, Biotechnol. Lett., 25 (2003) 1075-1080.
  • [22] Wang J., Krause R., Block K., Musameh M., Mulchandani A., Mulchandani P., Schöning M.J., Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chimica Acta, 469(2) (2002) 197–203.
  • [23] Dragonov D.I., Ladu B.N., Pharmacogenetics of paraoxonases: a brief review, Naunyn-Schmiedeberg’s Arch Pharmocol, 369(1) (2004) 78-88.
  • [24] Yağmuroğlu O., Diltemiz S.E., Development of QCM based biosensor for the selective and sensitive detection of paraoxon, Analytical Biochemistry, 591 (2020) 113572.
There are 24 citations in total.

Details

Primary Language English
Journal Section Natural Sciences
Authors

Ozan Yağmuroğlu 0000-0002-4703-6313

Sibel Emir Diltemiz 0000-0002-8627-6934

Publication Date December 29, 2020
Submission Date April 22, 2020
Acceptance Date October 20, 2020
Published in Issue Year 2020Volume: 41 Issue: 4

Cite

APA Yağmuroğlu, O., & Emir Diltemiz, S. (2020). Development of acetylcholinesterase immobilized CMD (Carboxymethyldextran) chip-based sensor for the detection of nerve agent simulant parathion. Cumhuriyet Science Journal, 41(4), 815-825. https://doi.org/10.17776/csj.725122