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Thymbra spicata Esansiyel Yağının Çok İlaç Dirençli Pseudomonas aeruginosa Suşlarına Antibakteriyel ve Antibiyofilm Etkisinin Araştırılması

Year 2018, Volume: 39 Issue: 3, 650 - 657, 30.09.2018

Uğur Tutar

https://doi.org/10.17776/csj.410749
Cited By: 1

Abstract

Thymbra spicata Anadolu'da astım, bronşit, solunum, öksürük ve
boğaz ağrısı enfeksiyonu gibi bazı hastalıkların tedavisi için geleneksel
olarak bitki çayı olarak kullanılmaktadır. Bu çalışmada, T. spicata esansiyel yağının (EOTS) antimikrobiyal, anti-biyofilm
aktivitesi ve kimyasal bileşimi araştırılmıştır. T. spicata' nın kimyasal bileşimi, gaz kromatografisi-kütle
spektrometresi (GC-MS) ile analiz edilmiştir. Antimikrobiyal ve anti-biyofilm
özellikleri, broth mikro-dilüsyon yöntemleri kullanılarak belirlenmiştir. Bu
çalışmanın sonuçlarına göre, GC-MS profili, ana bileşiklerin % 21,99
grandisol,% 6.45 cadinol,% 4.39 lavandulol asetat olduğu görülmektedir. EOTS
antibakteriyel aktivitesi, çoklu ilaç dirençli P. aeruginosa'ya karşı mikro-dilüsyon yöntemi ile değerlendirilmiştir.
EOTS’un test edilen suşlara karşı inhibitör etkiler ürettiği görülmektedir.
Esansiyel yağ, 11 adet P. aeruginosa klinik
izolatı, 1.56-12.5 μl / mL aralığında Minimum inhibitör Konsantrasyon (MIC)
değerleri ile inhibe etmiştir. Minimum Biyofilm İnhibitör Konsantrasyon (MBIC)
değeri 3.12-25 (μl / mL), Minimum Biyofilm Eradikasyon Konsantrasyonu (MBEC)
değeri ise 6.25-25 (μl / mL) olarak bulunmuştur. EOTS’ nin MIC değeri bakteri
biyofilm oluşumlarına uygulanmıştır. Biyofilm metabolizmasının % 2.0-51.6 arasında
azaldığı görülmüştür. Bu çalışmanın bulguları, EOTS'un P. aeruginosa izolatlarına karşı antibakteriyel ve anti-biyofilm
aktivitesi olduğunu göstermektedir. Bu nedenle, T. spicata esansiyel yağı çok ilaca dirençli P. aeruginosa enfeksiyonları ve biyofilmlerin tedavisi için
kullanılabilir potansiyel bir maddedir.

Keywords

Thymbra spicata Pseudomonas aeruginosa Antibakteriyel aktivite Antibiyofilm aktivite

References

  • [1]. Payne D.E., Boles B.R., Emerging interactions between matrix components during biofilm development, Current Genetics., 62 (2016) 137-141.
  • [2]. Lister J.L., Horswil A.R., Staphylococcus aureus biofilms: recent developments in biofilm dispersal, Frontiers in Cellular and Infection Microbiology., 4 (2014) 178.
  • [3]. Guvensen N.C., Ugur A., Okmen G., Examination of quorum-sensing responses of biocide-resistant biofilm bacteria isolated from a wastewater treatment system in industrial environment, Fresenius Environmental Bulletin., 26 (2017) 5021-5030.
  • [4]. McCarthy H., Rudkin J.K., Black N.S., Gallagher L., O'Neill E., O'Gara J.P., Methicillin resistance and the biofilm phenotype in Staphylococcus aureus, Frontiers in Cellular and Infection Microbiology., 5 (2015) 1.
  • [5]. Rasamiravaka T., Labtani Q., Duez P., El Jaziri M., The formation of biofilms by pseudomonas aeruginosa: a review of the natural and synthetic compounds ınterfering with control mechanisms, Biomed Res Int., 2015 (2015) 759348.
  • [6]. Daneshvar-Royandezagh S., Khawar K.M., Ozcan S., In vitro micropropagation of garden thyme (Thymbra spicata L. var. Spicata L.) collected from Southeastern Turkey using cotyledon node, Biotechnol Biotechnol Eq., 23 (2009) 1319-1321.
  • [7]. Marković T., Chatzopoulou P., Šiljegović J., Nikolic M., Glamo J., Ćirić A., Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components, Arch Biol Sci., 63 (2011) 457-44.
  • [8]. Dirican E., Turkez H., Toğar B., Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro, Cytotechnology., 64 (2012) 181-186.
  • [9]. Aksit H., Demirtas I., Telci I., Tarimcilar I., Chemical diversity in essential oil composition of Mentha longifolia (L.) Hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey, Journal of Essential Oil Research., 25-5 (2013) 430-437.
  • [10]. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24. Wayne, PA. 2014.
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  • [12]. Adukwu E.C, Allen S.C., Phillips C.A., The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus, Journal of Applied Microbiology., 113-5 (2012) 1217-1227.
  • [13]. Jardak M., Elloumi-Mseddi J., Aifa S., Mnif S., Chemical composition, anti-biofilm activity and potential cytotoxic effect on cancer cells of Rosmarinus officinalis L. essential oil from Tunisia, Lipids in Health and Disease., 16 (2017) 190.
  • [14]. Chaieb K., Kouidhi B., Jrah H., Mahdouani K., Bakhrouf A., Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation, BMC Complementary and Alternative Medicine., 11 (2011) 29.
  • [15]. Unlü M., Vardar U.G., Vural N., Dönmez E., Ozbaş Z.Y., Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey, Nat Prod Res., 23-6 (2009) 572-579.
  • [16]. Kiliç T., Analysis of essential oil composition of Thymbra spicata var. spicata: antifungal, antibacterial and antimycobacterial activities, Z Naturforsch C., 61-5 (2006) 324-328.
  • [17]. Kızıl S., Toncer O., Dıraz E., Karaman S., Variation of agronomical characteristics and essential oil components of zahter (Thymbra spicata L. var. spicata) populations in semi-arid climatic conditions, Turk J Field Crops ., 20-2 (2015) 242-251.
  • [18]. Baytok E., Kara K., Aksu T., Güçlü B.K., Özkaya S., Denek N., Kamalak A., Kaya D.A., Önel S.E., Akçay A., The effect of Mediterranean thyme (Thymbra spicata L. var. spicata) essential oil on fattening performance and ruminal parameters in lamb, Journal of Animal and Feed Sciences., 26 (2017) 319-325.
  • [19]. Al Hafi M., El Beyrouthy M., Ouaini N., Stien D., Rutledge D., Chaillou S., Chemical Composition and antimicrobial activity of Satureja, Thymus, and Thymbra Species grown in Lebanon, Chem. Biodiversity., 14 (2017) e1600236.
  • [20]. Pandey R.R., Dubey R.C., Saini S., Phytochemical and antimicrobial studies on essential oils of some aromatic plants, African Journal of Biotechnology., 9-28 (2010) 4364-4368.
  • [21]. Sarac N., Ugur A., Duru M.E., Antimicrobial activity and chemical composition of the essential oils of Thymbra spicata var. intricata, International Journal of Green Pharmacy., (2009) 24-28.
  • [22]. Mulcahy L.R., Isabella V.M., Lewis K., Pseudomonas aeruginosa biofilms in disease, Microb Ecol., 68-1 (2014) 1-12.

Investigation of Antibacterial and Anti-biofilm Activity of Thymbra spicata Essential Oil on Multidrug- Resistant Pseudomonas aeruginosa Strains

Year 2018, Volume: 39 Issue: 3, 650 - 657, 30.09.2018

Uğur Tutar

https://doi.org/10.17776/csj.410749
Cited By: 1

Abstract

Thymbra spicata has been used traditionally as herbal tea for
the treatment of some diseases such as asthma, bronchitis, respiratory, coughs,
and sore throat infection in Anatolia. In the current study, the essential oil
of T. spicata (EOTS) were
investigated for their antimicrobial, anti-biofilm activity and chemical
composition. The chemical composition of Thymbra
spicata was analyzed by gas chromatography-mass spectrometry (GC-MS).
Antimicrobial and anti-biofilm properties were determined using broth
microdilution methods. According to the results of the present study, the GC-MS
profile highlights that the mains compounds were found grandisol 21.99%,
cadinol 6.45%, lavandulol acetate 4.39%. The antibacterial activity of the EOTS
was evaluated by the micro-dilution method against multidrug resistant Pseudomonas aeruginosa. EOTS produced
inhibitory effects against the tested strains. It inhibited 11 clinical
isolates of against P. aeruginosa
with Minimum Inhibitory Concentration (MIC) values in the range of 1.56-12.5
μl/mL. Minimum Biofilm Inhibitory Concentration (MBIC) value was found to be
range of 3.12-25 (μl/mL) while the Minimum Biofilm Eradication Concentration
(MBEC) value was found to be 6.25-25 (μl/mL). MIC value of the EOTS was applied
onto the bacterial biofilm formations. It was seen that biofilm metabolism was
reduced by 2.0-51.6 %. The findings of this study show that EOTS has
antibacterial and anti-biofilm activity against P. aeruginosa isolates. Thus, essential oil of T. spicata may be useful a potential source for the treatment of
multidrug resistant P. aeruginosa
infections and biofilms.

Keywords

Thymbra spicata Pseudomonas aeruginosa Antibacterial activity Anti-biofilm activity

References

  • [1]. Payne D.E., Boles B.R., Emerging interactions between matrix components during biofilm development, Current Genetics., 62 (2016) 137-141.
  • [2]. Lister J.L., Horswil A.R., Staphylococcus aureus biofilms: recent developments in biofilm dispersal, Frontiers in Cellular and Infection Microbiology., 4 (2014) 178.
  • [3]. Guvensen N.C., Ugur A., Okmen G., Examination of quorum-sensing responses of biocide-resistant biofilm bacteria isolated from a wastewater treatment system in industrial environment, Fresenius Environmental Bulletin., 26 (2017) 5021-5030.
  • [4]. McCarthy H., Rudkin J.K., Black N.S., Gallagher L., O'Neill E., O'Gara J.P., Methicillin resistance and the biofilm phenotype in Staphylococcus aureus, Frontiers in Cellular and Infection Microbiology., 5 (2015) 1.
  • [5]. Rasamiravaka T., Labtani Q., Duez P., El Jaziri M., The formation of biofilms by pseudomonas aeruginosa: a review of the natural and synthetic compounds ınterfering with control mechanisms, Biomed Res Int., 2015 (2015) 759348.
  • [6]. Daneshvar-Royandezagh S., Khawar K.M., Ozcan S., In vitro micropropagation of garden thyme (Thymbra spicata L. var. Spicata L.) collected from Southeastern Turkey using cotyledon node, Biotechnol Biotechnol Eq., 23 (2009) 1319-1321.
  • [7]. Marković T., Chatzopoulou P., Šiljegović J., Nikolic M., Glamo J., Ćirić A., Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components, Arch Biol Sci., 63 (2011) 457-44.
  • [8]. Dirican E., Turkez H., Toğar B., Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro, Cytotechnology., 64 (2012) 181-186.
  • [9]. Aksit H., Demirtas I., Telci I., Tarimcilar I., Chemical diversity in essential oil composition of Mentha longifolia (L.) Hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey, Journal of Essential Oil Research., 25-5 (2013) 430-437.
  • [10]. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24. Wayne, PA. 2014.
  • [11]. Ceri H., Olson M.E., Morck D.V., Storey D.G., Minimal biofilm eradication (MBEC) assay: susceptibility testing for biofilms. In: Pace JL, Rupp ME, Finch RG, editors. Biofilms, infection, and antimicrobial theraphy. CRC Pres, Boca Raton, 2006; pp 257-269.
  • [12]. Adukwu E.C, Allen S.C., Phillips C.A., The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus, Journal of Applied Microbiology., 113-5 (2012) 1217-1227.
  • [13]. Jardak M., Elloumi-Mseddi J., Aifa S., Mnif S., Chemical composition, anti-biofilm activity and potential cytotoxic effect on cancer cells of Rosmarinus officinalis L. essential oil from Tunisia, Lipids in Health and Disease., 16 (2017) 190.
  • [14]. Chaieb K., Kouidhi B., Jrah H., Mahdouani K., Bakhrouf A., Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation, BMC Complementary and Alternative Medicine., 11 (2011) 29.
  • [15]. Unlü M., Vardar U.G., Vural N., Dönmez E., Ozbaş Z.Y., Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey, Nat Prod Res., 23-6 (2009) 572-579.
  • [16]. Kiliç T., Analysis of essential oil composition of Thymbra spicata var. spicata: antifungal, antibacterial and antimycobacterial activities, Z Naturforsch C., 61-5 (2006) 324-328.
  • [17]. Kızıl S., Toncer O., Dıraz E., Karaman S., Variation of agronomical characteristics and essential oil components of zahter (Thymbra spicata L. var. spicata) populations in semi-arid climatic conditions, Turk J Field Crops ., 20-2 (2015) 242-251.
  • [18]. Baytok E., Kara K., Aksu T., Güçlü B.K., Özkaya S., Denek N., Kamalak A., Kaya D.A., Önel S.E., Akçay A., The effect of Mediterranean thyme (Thymbra spicata L. var. spicata) essential oil on fattening performance and ruminal parameters in lamb, Journal of Animal and Feed Sciences., 26 (2017) 319-325.
  • [19]. Al Hafi M., El Beyrouthy M., Ouaini N., Stien D., Rutledge D., Chaillou S., Chemical Composition and antimicrobial activity of Satureja, Thymus, and Thymbra Species grown in Lebanon, Chem. Biodiversity., 14 (2017) e1600236.
  • [20]. Pandey R.R., Dubey R.C., Saini S., Phytochemical and antimicrobial studies on essential oils of some aromatic plants, African Journal of Biotechnology., 9-28 (2010) 4364-4368.
  • [21]. Sarac N., Ugur A., Duru M.E., Antimicrobial activity and chemical composition of the essential oils of Thymbra spicata var. intricata, International Journal of Green Pharmacy., (2009) 24-28.
  • [22]. Mulcahy L.R., Isabella V.M., Lewis K., Pseudomonas aeruginosa biofilms in disease, Microb Ecol., 68-1 (2014) 1-12.
There are 22 citations in total.

Details

Primary Language English
Journal Section Natural Sciences
Authors

Uğur Tutar

Publication Date September 30, 2018
Submission Date March 29, 2018
Acceptance Date July 6, 2018
Published in Issue Year 2018Volume: 39 Issue: 3

Cite

APA Tutar, U. (2018). Investigation of Antibacterial and Anti-biofilm Activity of Thymbra spicata Essential Oil on Multidrug- Resistant Pseudomonas aeruginosa Strains. Cumhuriyet Science Journal, 39(3), 650-657. https://doi.org/10.17776/csj.410749

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