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Determination of Cytotoxic Activity of Aronia melanocarpa (Michx.) Elliot Fruit Extracts on Breast Cancer (MCF-7) and Cervical Cancer (HeLa) Cell Lines

Year 2024, , 537 - 542, 30.09.2024

Eda Sönmez Gürer , Ayhan Altıntaş

https://doi.org/10.17776/csj.1497258

Abstract

Aronia (chokeberry) fruits are consumed as fresh fruit due to their high antioxidant activity, and are also preferred among the public in the production of natural medicines. Aronia melanocarpa (Michx.) Elliot, a species of the Rosaceae family, contains many phytochemical compounds such as flavonoids, phenolic compounds, lignans, terpenes, tocopherols, phospholipids, organic acids and high amounts of anthocyanins. In this study, it was aimed to determine the in vitro anticarcinogenic activities of A. melanocarpa (Michx.) Elliot fruit extracts prepared with 6 different solvents. In the study, the cytotoxic effects of the fruits were investigated using breast cancer cell line (MCF-7) and cervical cancer cell line (HeLa), and their effects on healthy cells were investigated using human endothelial cells (HUVEC) and mouse fibroblast cells (L929) by the MTT method. As a result of the study; It was determined that the highest cytotoxicity on the breast cancer (MCF-7) cell line was observed in the ethanol extract (IC50=111.44 µg/mL) and the lowest cytotoxicity was observed in the hexane extract (IC50=661.80 µg/mL). It was determined that the highest cytotoxicity on the cervical cancer (HeLa) cell line was observed in the ethanol extract (IC50=95.14 µg/mL) and the lowest cytotoxicity was observed in the ethyl acetate extract (IC50=319.51 µg/mL). According to these values; It was determined that all extracts of Aronia fruit had cytotoxic effects on MCF-7 and HeLa cell lines, selectivity index values were higher in HeLa cells, and they did not have cytotoxic effects on HUVEC and L929 healthy cell lines (IC50=411.25-663.27 µg/mL). Thus, it has been determined that the fruits of the Aronia melanocarpa species are promising in the development of new natural resources, new drugs and therapeutic agents in cancer treatment, thanks to their anticancer activities and low cytotoxicity. It is recommended that further research be conducted on the mechanisms of anticancer activity in the future

Keywords

Aronia melanocarpa Antioxidant Anticancer Cytotoxicity

References

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  • [5] Çağlar M.Y., Demirci M., Üzümsü Meyvelerde Bulunan Fenolik Bileşikler ve Beslenmedeki Önemi, Avrupa Bilim ve Teknoloji Dergisi, 7 (2017) 18–26.
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  • [9] Kaloudi T., Tsimogiannis D., Oreopoulou V., Aronia melanocarpa: Identification and Exploitation of its Phenolic Components, Molecules, 27 (2022) 4375.
  • [10] Kulling S., Rawel H., Chokeberry (Aronia melanocarpa) – A Review on the Characteristic Components and Potential Health Effects, Planta Med., 74 (2008) 1625–1634.
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  • [14] Saraç H., Daştan T., Durukan H., Durna Daştan S., Demirbaş A., Karaköy T., Kırmızı Gelincik (Fam: Papaveraceae, Glaucium grandiflorum Boiss.&Huet var. grandiflorum) Bitkisinin Farklı Özütlerinin Besin Elementi İçeriğinin ve In Vitro Antiproliferatif Etkilerinin Değerlendirilmesi, Ziraat Fakültesi Dergisi, (2018) 417–428.
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  • [19] Sharif T., Stambouli M., Burrus B., Emhemmed F., Dandache I., Auger C., The Polyphenolic-Rich Aronia melanocarpa Juice Kills Teratocarcinomal Cancer Stem-Like Cells, but Not Their Differentiated Counterparts, J Funct Foods, 5 (2013) 1244–1252.
  • [20] Efenberger-Szmechtyk M., Nowak A., Nowak A., Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2, Antioxidants, 9 (2020) 1030.
  • [21] McDougall G.J., Ross H.A., Ikeji M., Stewart D., Berry Extracts Exert Different Antiproliferative Effects against Cervical and Colon Cancer Cells Grown In Vitro, J Agric Food Chem., 56 (2008) 3016–3023.
  • [22] Konić-Ristić A., Šavikin K., Zdunić G., Janković T., Juranic Z., Menković N., Biological Activity and Chemical Composition of Different Berry Juices, Food Chem., 125 (2011) 1412–1417.
  • [23] Rugină D., Sconţa Z., Leopold L., Pintea A., Bunea A., Socaciu C., Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells, J. Med. Food, 15 (2012) 700–706.
  • [24] Thani N.A.A., Keshavarz S., Lwaleed B.A., Cooper A.J., Rooprai H.K., Cytotoxicity of Gemcitabine Enhanced by Polyphenolics from Aronia melanocarpa in Pancreatic Cancer Cell Line AsPC-1, J. Clin. Pathol., 67 (2014) 949–954.
  • [25] Kedzierska M., Olas B., Wachowicz B., Stochmal A., Oleszek W., Jeziorski A., The Nitrative and Oxidative Stress in Blood Platelets İsolated from Breast Cancer Patients: The Protectory Action of Aronia melanocarpa Extract, Platelets, 21 (2010) 541–548.
  • [26] Bermudez-Soto M., Tomasbarberan F., Garciaconesa M., Stability of Polyphenols in Chokeberry (Aronia melanocarpa) Subjected to In Vitro Gastric and Pancreatic Digestion, Food Chem., 102 (2007) 865–874.
  • [27] Gao N., Wang Y., Jiao X., Chou S., Li E., Li B., Preparative Purification of Polyphenols from Aronia melanocarpa (Chokeberry) with Cellular Antioxidant and Antiproliferative Activity, Molecules, 23 (2018) 139.
  • [28] Caliskan Z., Yucel M.F., Celikok Y., Guler V., Duranay S. A., Preliminary Study of the Anti-Proliferative Effect of Aronia melanocarpa Extract on Human Colon Cancer Cells and its Relation with Human TERT Protein, Experimental Biomedical Research, 6(2) (2023) 88-98.
  • [29] Cvetanović A., Zengin G., Zeković Z., Švarc-Gajić J., Ražić S., Damjanović A., Comparative In Vitro Studies of the Biological Potential and Chemical Composition of Stems, Leaves and Berries Aronia melanocarpa’s Extracts Obtained by Subcritical Water Extraction, Food and Chemical Toxicology, 121 (2018) 458–466.
  • [30] Šavikin K., Zdunić G., Janković T., Gođevac D., Stanojković T., Pljevljakušić D., Berry Fruit Teas: Phenolic Composition and Cytotoxic Activity, Food Research International, 62 (2014) 677–683.

Year 2024, , 537 - 542, 30.09.2024

Eda Sönmez Gürer , Ayhan Altıntaş

https://doi.org/10.17776/csj.1497258

Abstract

References

  • [1] Jurikova T., Mlcek J., Skrovankova S., Sumczynski D., Sochor J., Hlavacova I., Fruits of Black Chokeberry Aronia melanocarpa in the Prevention of Chronic Diseases, Molecules, 22 (2017) 944.
  • [2] Gorzynik-Debicka M., Przychodzen P., Cappello F., Kuban-Jankowska A., Marino Gammazza A., Knap N., Potential Health Benefits of Olive Oil and Plant Polyphenols, Int J Mol Sci., 19 (2018) 686.
  • [3] Şekeroğlu Z.A., Şekeroğlu V., Oksidatif Mitokondrial Hasar ve Yaşlanmadaki Önemi, Türk Bilimsel Derleme Dergisi, 2 (2009) 69–74.
  • [4] Benvenuti S., Pelleti F., Melegari M., Bertelli D., Polyphenols, Anthocyanins, Ascorbic Acid and Radical Scavenging Activity of Rubus, Ribes, and Aronia, J Food Sci., 69(3) (2004) 164-169.
  • [5] Çağlar M.Y., Demirci M., Üzümsü Meyvelerde Bulunan Fenolik Bileşikler ve Beslenmedeki Önemi, Avrupa Bilim ve Teknoloji Dergisi, 7 (2017) 18–26.
  • [6] Ağaoğlu Y.S., Türkiye’de Üzümsü Meyvelerin Dünü Bugünü ve Yarını, Ulusal Kivi ve Üzümsü Meyveler Sempozyumu Ordu, (2003) 1–14.
  • [7] Akalın G., Selamoglu Z., Nutrition and Foods for Skin Health, Journal of Pharmaceutical Care, 7(1-2) (2019) 31-33
  • [8] Deng H., Zhu J., Tong Y., Kong Y., Tan C., Wang M., Antibacterial Characteristics and Mechanisms of Action of Aronia melanocarpa Anthocyanins Against Escherichia coli, LWT, 150 (2021) 112018.
  • [9] Kaloudi T., Tsimogiannis D., Oreopoulou V., Aronia melanocarpa: Identification and Exploitation of its Phenolic Components, Molecules, 27 (2022) 4375.
  • [10] Kulling S., Rawel H., Chokeberry (Aronia melanocarpa) – A Review on the Characteristic Components and Potential Health Effects, Planta Med., 74 (2008) 1625–1634.
  • [11] Średnicka-Tober D., Kazimierczak R., Rembiałkowska E., Strok T., Świąder K., Hallmann E., Bioactive Compounds in Organic Apple Juices Enriched with Chokeberry and Green Tea Extract, Journal of Research and Applications in Agricultural Engineering, 62 (2017) 173–177.
  • [12] Cancer in World Health Organization Available at: http://www.who.int/news-room/fact-sheets/detail , Retrieved January, 2024.
  • [13] Saraç H., Daştan T., Demirbaş A., Durna Daştan S., Karaköy T., Durukan H., Madımak (Polygonum cognatum Meissn.) Bitki Özütlerinin Besin Elementleri ve İn Vitro Antikanserojen Aktiviteleri Yönünden Değerlendirilmesi, Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 1. Uluslararası Tarımsal Yapılar ve Sulama Kongresi, (2018) 340–347.
  • [14] Saraç H., Daştan T., Durukan H., Durna Daştan S., Demirbaş A., Karaköy T., Kırmızı Gelincik (Fam: Papaveraceae, Glaucium grandiflorum Boiss.&Huet var. grandiflorum) Bitkisinin Farklı Özütlerinin Besin Elementi İçeriğinin ve In Vitro Antiproliferatif Etkilerinin Değerlendirilmesi, Ziraat Fakültesi Dergisi, (2018) 417–428.
  • [15] Al-Qubaisi M., Rozita R., Yeap S.K., Omar A.R., Ali A.M., Alitheen N.B., Selective Cytotoxicity of Goniothalamin Against Hepatoblastoma HepG2 Cells, Molecules, 16 (2011) 2944–2959.
  • [16] Tokur O., Aksoy A., In Vitro Sitotoksisite Testleri, Harran Üniversitesi Veteriner Fakültesi Dergisi, 6 (2017) 112–118.
  • [17] Mosmann T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays, J Immunol Methods, 65 (1983) 55–63.
  • [18] Riss T.L., Moravec R.A., Use of Multiple Assay Endpoints to Investigate the Effects of Incubation Time, Dose of Toxin, and Plating Density in Cell-Based Cytotoxicity Assays, Assay Drug Dev Technol, 2 (2004) 51–62.
  • [19] Sharif T., Stambouli M., Burrus B., Emhemmed F., Dandache I., Auger C., The Polyphenolic-Rich Aronia melanocarpa Juice Kills Teratocarcinomal Cancer Stem-Like Cells, but Not Their Differentiated Counterparts, J Funct Foods, 5 (2013) 1244–1252.
  • [20] Efenberger-Szmechtyk M., Nowak A., Nowak A., Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2, Antioxidants, 9 (2020) 1030.
  • [21] McDougall G.J., Ross H.A., Ikeji M., Stewart D., Berry Extracts Exert Different Antiproliferative Effects against Cervical and Colon Cancer Cells Grown In Vitro, J Agric Food Chem., 56 (2008) 3016–3023.
  • [22] Konić-Ristić A., Šavikin K., Zdunić G., Janković T., Juranic Z., Menković N., Biological Activity and Chemical Composition of Different Berry Juices, Food Chem., 125 (2011) 1412–1417.
  • [23] Rugină D., Sconţa Z., Leopold L., Pintea A., Bunea A., Socaciu C., Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells, J. Med. Food, 15 (2012) 700–706.
  • [24] Thani N.A.A., Keshavarz S., Lwaleed B.A., Cooper A.J., Rooprai H.K., Cytotoxicity of Gemcitabine Enhanced by Polyphenolics from Aronia melanocarpa in Pancreatic Cancer Cell Line AsPC-1, J. Clin. Pathol., 67 (2014) 949–954.
  • [25] Kedzierska M., Olas B., Wachowicz B., Stochmal A., Oleszek W., Jeziorski A., The Nitrative and Oxidative Stress in Blood Platelets İsolated from Breast Cancer Patients: The Protectory Action of Aronia melanocarpa Extract, Platelets, 21 (2010) 541–548.
  • [26] Bermudez-Soto M., Tomasbarberan F., Garciaconesa M., Stability of Polyphenols in Chokeberry (Aronia melanocarpa) Subjected to In Vitro Gastric and Pancreatic Digestion, Food Chem., 102 (2007) 865–874.
  • [27] Gao N., Wang Y., Jiao X., Chou S., Li E., Li B., Preparative Purification of Polyphenols from Aronia melanocarpa (Chokeberry) with Cellular Antioxidant and Antiproliferative Activity, Molecules, 23 (2018) 139.
  • [28] Caliskan Z., Yucel M.F., Celikok Y., Guler V., Duranay S. A., Preliminary Study of the Anti-Proliferative Effect of Aronia melanocarpa Extract on Human Colon Cancer Cells and its Relation with Human TERT Protein, Experimental Biomedical Research, 6(2) (2023) 88-98.
  • [29] Cvetanović A., Zengin G., Zeković Z., Švarc-Gajić J., Ražić S., Damjanović A., Comparative In Vitro Studies of the Biological Potential and Chemical Composition of Stems, Leaves and Berries Aronia melanocarpa’s Extracts Obtained by Subcritical Water Extraction, Food and Chemical Toxicology, 121 (2018) 458–466.
  • [30] Šavikin K., Zdunić G., Janković T., Gođevac D., Stanojković T., Pljevljakušić D., Berry Fruit Teas: Phenolic Composition and Cytotoxic Activity, Food Research International, 62 (2014) 677–683.
There are 30 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry, Cancer Biology
Journal Section Natural Sciences
Authors

Eda Sönmez Gürer 0000-0003-0319-6312

Ayhan Altıntaş 0000-0002-9413-0636

Publication Date September 30, 2024
Submission Date June 7, 2024
Acceptance Date September 23, 2024
Published in Issue Year 2024

Cite

APA Sönmez Gürer, E., & Altıntaş, A. (2024). Determination of Cytotoxic Activity of Aronia melanocarpa (Michx.) Elliot Fruit Extracts on Breast Cancer (MCF-7) and Cervical Cancer (HeLa) Cell Lines. Cumhuriyet Science Journal, 45(3), 537-542. https://doi.org/10.17776/csj.1497258