Multispectral Imaging and Single-Cell Analysis with Genetically Encoded Biosensors Unveil Complex Interactions between Extracellular ATP and Intracellular Calcium

Emrah Eroğlu

doi:10.17776/csj.1462654

Research Article

Multispectral Imaging and Single-Cell Analysis with Genetically Encoded Biosensors Unveil Complex Interactions between Extracellular ATP and Intracellular Calcium

Year 2024, , 360 - 365, 30.06.2024

Emrah Eroğlu

https://doi.org/10.17776/csj.1462654

Abstract

The dynamic interplay between extracellular ATP (eATP) and intracellular calcium ([Ca2+]i) serves as a pivotal signaling axis in cellular physiology, influencing a myriad of cellular processes. Traditionally recognized as an energy currency within the cell, ATP has emerged as a multifunctional signaling molecule that orchestrates diverse cellular responses through activation of purinergic receptors. The complex link between ATP signaling and calcium dynamics plays a central role in cellular communication and homeostasis. Advancements in imaging technologies such as development of genetically encoded biosensors have revolutionized the study of cellular signaling dynamics, enabling visualization of the spatiotemporal aspects of eATP and [Ca2+]i in real-time. The convergence of eATP signaling and [Ca2+]i dynamics serves as a central hub in cellular communication. In this study, utilizing bicistronic construct biosensors for multispectral imaging of [Ca2+]i responses to eATP, we show that distinct concentrations of eATP administration reveal complex intracellular [Ca2+]i responses, potentially attributed to receptor desensitization. Single-cell co-imaging uncovers [Ca2+]i heterogeneity, emphasizing the significance of individual cell dynamics in eATP-induced calcium signaling. Therefore, this study sheds light on the intricacies of eATP-induced calcium signaling, providing insights valuable for basic research and therapeutic applications.

Keywords

Calcium signaling, ATP signaling, Single cell imaging, Genetically encoded eiosensors, Multispectral imaging

Ethical Statement

N.A.

Project Number

EMBO IG-5322-2023

Thanks

This research was supported by funds from the European Molecular Biology Organization EMBO (IG-5322-2023. The author acknowledges Asal Ghaffari Zaki for English language proofreading. Figure 1A was generated with Biorender (Licence code: ME26EKR3WY).

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