Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells

Murat Doğan; Kenan Yıldızhan

EN

Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells

Abstract

Paracetamol is an active metabolite with analgesic and antipyretic properties of phenacetin, which is sold without a prescription in our country and in many countries. However, the effect of paracetamol on oxidative stress due to glutamate-induced cytotoxicity remains unclear. This study aims to investigate the effect of an appropriate dose of paracetamol on nitric oxide and increased oxidative stress as a result of glutamate-induced cytotoxicity in C6 cells. The cells were divided into 4 groups as Control group, Glutamate group, Paracetamol group, and Paracetamol+Glutamate group. Cell viability rate between groups was measured by XTT assay. Oxidative stress and antioxidant levels were measured with TOS and TAS elisa kits. Paracetamol at all concentrations significantly increased cell viability in C6 cells (p < 0.001). Paracetamol also increased TAS levels (p <0.01) while significantly decreased TOS levels (p < 0.001). In addition, paracetamol was observed to decrease TNF-α and NO levels (p <0.001). In conclusion, paracetamol has protective feature on glutamate-induced cytotoxicity in C6 glial cells by suppressing oxidative stress. The results of this study show that when the appropriate dose of paracetamol is used, it can be a crucial promoter agent in glutamate toxicity-induced neurodegeneration.

Keywords

Thanks

The authors would like to thank the CUTFAM Research Center, Sivas Cumhuriyet University, School of Medicine, Sivas, Turkey, for providing the necessary facilities to conduct this study.

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Murat Doğan ^*
0000-0003-2794-0177
Türkiye

Kenan Yıldızhan
0000-0002-6585-4010
Türkiye

Publication Date

December 29, 2021

Submission Date

September 23, 2021

Acceptance Date

December 4, 2021

Published in Issue

Year 2021 Volume: 42 Number: 4

IZ

https://izlik.org/JA35GN48KF

Cite

RIS / Bibtex

APA

Doğan, M., & Yıldızhan, K. (2021). Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells. Cumhuriyet Science Journal, 42(4), 789-794. https://izlik.org/JA35GN48KF

AMA

1.Doğan M, Yıldızhan K. Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells. CSJ. 2021;42(4):789-794. https://izlik.org/JA35GN48KF

Chicago

Doğan, Murat, and Kenan Yıldızhan. 2021. “Investigation of the Effect of Paracetamol Against Glutamate-Induced Cytotoxicity in C6 Glia Cells”. Cumhuriyet Science Journal 42 (4): 789-94. https://izlik.org/JA35GN48KF.

EndNote

Doğan M, Yıldızhan K (December 1, 2021) Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells. Cumhuriyet Science Journal 42 4 789–794.

IEEE

[1]M. Doğan and K. Yıldızhan, “Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells”, CSJ, vol. 42, no. 4, pp. 789–794, Dec. 2021, [Online]. Available: https://izlik.org/JA35GN48KF

ISNAD

Doğan, Murat - Yıldızhan, Kenan. “Investigation of the Effect of Paracetamol Against Glutamate-Induced Cytotoxicity in C6 Glia Cells”. Cumhuriyet Science Journal 42/4 (December 1, 2021): 789-794. https://izlik.org/JA35GN48KF.

JAMA

1.Doğan M, Yıldızhan K. Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells. CSJ. 2021;42:789–794.

MLA

Doğan, Murat, and Kenan Yıldızhan. “Investigation of the Effect of Paracetamol Against Glutamate-Induced Cytotoxicity in C6 Glia Cells”. Cumhuriyet Science Journal, vol. 42, no. 4, Dec. 2021, pp. 789-94, https://izlik.org/JA35GN48KF.

Vancouver

1.Murat Doğan, Kenan Yıldızhan. Investigation of the effect of paracetamol against glutamate-induced cytotoxicity in C6 glia cells. CSJ [Internet]. 2021 Dec. 1;42(4):789-94. Available from: https://izlik.org/JA35GN48KF