Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Derya Kızıloluk; Gültekin Gökçe; Şenay Akkuş Çetinus

EN

Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Abstract

In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action. Thus, studies to examine interactions with DNA using biosensors have gained momentum. In our study, it was investigated that Capecitabine (CPT), an anticancer drug, and glassy carbon electrode (GCE) interaction by using electrochemical methods. The interaction of CPT with calf thymus DNAs (dsDNA, ssDNA) immobilized on the electrode surface was analyzed by exploiting changes in the oxidation signals of the guanine base. The immobilization of DNA on the electrode surface has been optimized. Optimal DNA concentration and optimal interaction times were found. Electrochemical impedance spectroscopy technique was used for impedimetric measurements.
The results obtained confirmed that the ct-DNAs were immobilized on the electrode surface. Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Derya Kızıloluk ^*
0000-0002-0896-6550
Türkiye

Gültekin Gökçe
0000-0003-1676-194X
Türkiye

Şenay Akkuş Çetinus
0000-0001-5705-6839
Türkiye

Publication Date

December 29, 2021

Submission Date

June 17, 2021

Acceptance Date

October 3, 2021

Published in Issue

Year 2021 Volume: 42 Number: 4

IZ

https://izlik.org/JA93KH56ZX

Cite

RIS / Bibtex

APA

Kızıloluk, D., Gökçe, G., & Akkuş Çetinus, Ş. (2021). Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). Cumhuriyet Science Journal, 42(4), 814-821. https://izlik.org/JA93KH56ZX

AMA

1.Kızıloluk D, Gökçe G, Akkuş Çetinus Ş. Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). CSJ. 2021;42(4):814-821. https://izlik.org/JA93KH56ZX

Chicago

Kızıloluk, Derya, Gültekin Gökçe, and Şenay Akkuş Çetinus. 2021. “Electrochemical Investigation of DNA and Capecitabine Interaction Using Glassy Carbon Electrode (GCE)”. Cumhuriyet Science Journal 42 (4): 814-21. https://izlik.org/JA93KH56ZX.

EndNote

Kızıloluk D, Gökçe G, Akkuş Çetinus Ş (December 1, 2021) Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). Cumhuriyet Science Journal 42 4 814–821.

IEEE

[1]D. Kızıloluk, G. Gökçe, and Ş. Akkuş Çetinus, “Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)”, CSJ, vol. 42, no. 4, pp. 814–821, Dec. 2021, [Online]. Available: https://izlik.org/JA93KH56ZX

ISNAD

Kızıloluk, Derya - Gökçe, Gültekin - Akkuş Çetinus, Şenay. “Electrochemical Investigation of DNA and Capecitabine Interaction Using Glassy Carbon Electrode (GCE)”. Cumhuriyet Science Journal 42/4 (December 1, 2021): 814-821. https://izlik.org/JA93KH56ZX.

JAMA

1.Kızıloluk D, Gökçe G, Akkuş Çetinus Ş. Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). CSJ. 2021;42:814–821.

MLA

Kızıloluk, Derya, et al. “Electrochemical Investigation of DNA and Capecitabine Interaction Using Glassy Carbon Electrode (GCE)”. Cumhuriyet Science Journal, vol. 42, no. 4, Dec. 2021, pp. 814-21, https://izlik.org/JA93KH56ZX.

Vancouver

1.Derya Kızıloluk, Gültekin Gökçe, Şenay Akkuş Çetinus. Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). CSJ [Internet]. 2021 Dec. 1;42(4):814-21. Available from: https://izlik.org/JA93KH56ZX