Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Şifa Türkoğlu

doi:10.17776/csj.863973

EN

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Abstract

In this study, the cytotoxic and genotoxic effects of Reactive Blue 19 and Reactive Black 5 were investigated using the Allium test and comet assay. These chemicals are fabric dyes used in textile industries in various parts of Turkey. Bulbs with roots of Allium cepa L. were treated with different concentrations (25, 50 and 100 ppm) of these textile dyes for 24 h. and 48 h. The root tips were processed for cytological studies by the aseto-orcein squash procedure. Distilled water and methyl methane sulfonate (MMS, 10 ppm) were used as an negative and positive control, respectively. Exposure of Reactive Blue 19 and Reactive Black 5 significantly decreased mitotic index values. Additionally, all treatments changed the frequency of mitotic phases when compared with the control groups. These dyes increased chromosome aberrations in test material. Among these abnormalities were anaphase bridges, c-mitosis, laggards, micronuclei and stickiness. A significant increase in DNA damage was also observed at all concentrations of both Reactive Blue 19 and Reactive Black 5 examined by comet assay.

Keywords

Thanks

Author is thankful to Dr. Gülben Albayrak for fluorescence microscope.

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Şifa Türkoğlu ^*
0000-0002-2725-9827
Türkiye

Publication Date

September 24, 2021

Submission Date

January 18, 2021

Acceptance Date

August 26, 2021

Published in Issue

Year 2021 Volume: 42 Number: 3

DOI

https://doi.org/10.17776/csj.863973

IZ

https://izlik.org/JA48UA66HZ

Cite

RIS / Bibtex

APA

Türkoğlu, Ş. (2021). Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator. Cumhuriyet Science Journal, 42(3), 515-525. https://doi.org/10.17776/csj.863973

Cited By

GC–MS and HPLC supported phytochemical analysis of watercress and the protective role against paraben toxicity

Environmental Science and Pollution Research

https://doi.org/10.1007/s11356-022-22380-7

Öz

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

GC–MS and HPLC supported phytochemical analysis of watercress and the protective role against paraben toxicity