Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey

Sinem Demir; Mehmet Ataş

doi:10.17776/csj.842900

EN

Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey

Abstract

Tularemia is a zoonotic disease that can infect animals and people. It is known that tularemia outbreaks in Turkey is water-borne and causative agent F. tularensis subsp. holarctica. Eight F. tularensis were isolated from the water samples in tularemia outbreaks observed in Sivas during 2011-2013. In this study; tul4, fopA, RD1 and 16S rRNA gene regions of eight F. tularensis isolates were amplified by PCR method and investigated by sequencing method. Sequence analysis of the gene regions were compared with each other and the samples found in GenBank. All samples were found to be similar in terms of the partial sequence of the tul4 and fopA gene region. In terms of 16S rRNA gene region, Belkent2012 and Belkent2013 isolates and Çiçekoğlu and Döllük isolates were detected similar among themselves. Bahçeiçi and Karaören isolates were found similar at RD1 gene regions. In the sequence comparison of GenBank in terms of examined gene regions, 94-100% similarity was determined with F. tularensis subsp. holarctica LVS and F. tularensis subsp. holarctica PHIT-FT049. As a result of our study; the tul4 and fopA gene regions of eight F. tularensis subsp. holarctica isolates were found to have identical and different base sequence origins in 16S rRNA and RD1 gene regions in tularemia outbreaks in Sivas. In order to reveal the phylogeography of F. tularensis’ in Turkey, it is necessary to produce new isolates from epidemic regions and to investigate them with advanced molecular techniques.

Keywords

Supporting Institution

This work was supported by the Scientific Research Project Fund of Sivas Cumhuriyet University (CÜBAP) under the project number ECZ-054.

Project Number

ECZ-054

Thanks

We would lik to thank Dr. Bekir Çelebi (Public Health Institution of Turkey, National Tularemia Reference Laboratory, Ankara, Turkey) for the F. tularensis subsp. holarctica LVS strain (NCTC 10857).

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Sinem Demir
0000-0002-3536-9735
Türkiye

Mehmet Ataş ^*
0000-0002-9425-0080
Türkiye

Publication Date

March 29, 2021

Submission Date

December 18, 2020

Acceptance Date

March 8, 2021

Published in Issue

Year 2021 Volume: 42 Number: 1

DOI

https://doi.org/10.17776/csj.842900

IZ

https://izlik.org/JA87GS57PH

Cite

RIS / Bibtex

APA

Demir, S., & Ataş, M. (2021). Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey. Cumhuriyet Science Journal, 42(1), 7-12. https://doi.org/10.17776/csj.842900

Öz

Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite