Ultrafiltration-Based extraction of Ibuprofen from human plasma samples and HPLC analysis: developing an innovative bioanalytical analysis method

Yasin Şenol; Ayşegül Doğan; Mustafa Çelebier

doi:10.17776/csj.839807

EN

Ultrafiltration-Based extraction of Ibuprofen from human plasma samples and HPLC analysis: developing an innovative bioanalytical analysis method

Abstract

Some commercially available scientific products for ultrafiltration are readily available and have been used for a long time, especially in environmental sciences, life sciences and analysis of active ingredients from plants. Recent studies of metabolomics discuss the advantage and disadvantage of ultrafiltration versus protein precipitation and solid phase extraction. However, ultrafiltration techniques are rarely used in pharmaceutical analysis, even though they have advantages over solid phase extraction, such as easy to apply and no buffer preparations. The aim of this study is to develop a simple and innovative analysis technique for IBU determination from human plasma samples by ultrafiltration-based extraction in a pharmaceutical analysis. the prepared samples were analyzed using HPLC system equipped with C18 Column (100 x 4.6 mm, 3.5 µm). The flow rate was set to 1.0 mL min-1 while using isocratic elution with the mobile phase of [acetonitrile: phosphate buffer (pH 3.0, 15 mM) (56:44 v/v)] solution. Injection volume was 20 μL and UV detection was performed at 220 nm wavelenght. The developed method was validated according to FDA Bioanalytical Guidelines and found accurate, precise, sensitive, selective and efficient with the high recovery values samples.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Yasin Şenol
0000-0001-5151-8027
Türkiye

Ayşegül Doğan ^*
0000-0002-5828-4697
Türkiye

Mustafa Çelebier
0000-0001-7712-5512
Türkiye

Publication Date

June 30, 2021

Submission Date

December 12, 2020

Acceptance Date

April 17, 2021

Published in Issue

Year 2021 Volume: 42 Number: 2

DOI

https://doi.org/10.17776/csj.839807

IZ

https://izlik.org/JA98FX34YX

Cite

RIS / Bibtex

APA

Şenol, Y., Doğan, A., & Çelebier, M. (2021). Ultrafiltration-Based extraction of Ibuprofen from human plasma samples and HPLC analysis: developing an innovative bioanalytical analysis method. Cumhuriyet Science Journal, 42(2), 276-284. https://doi.org/10.17776/csj.839807

Cited By

Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate

Scientia Pharmaceutica

https://doi.org/10.3390/scipharm90030053

AQbD framework for a chromatographic assay of six common analgesics in real human plasma samples: sustainability assessment

Microchemical Journal

https://doi.org/10.1016/j.microc.2025.116515

Öz

Ultrafiltration-Based extraction of Ibuprofen from human plasma samples and HPLC analysis: developing an innovative bioanalytical analysis method

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate

AQbD framework for a chromatographic assay of six common analgesics in real human plasma samples: sustainability assessment