The in vitro effect of 5-FU and Tamoxifen Chemotherapeutics on penthose phosphate pathway enzymes

Yusuf Temel

doi:10.17776/csj.806343

EN

The in vitro effect of 5-FU and Tamoxifen Chemotherapeutics on penthose phosphate pathway enzymes

Abstract

The pentose phosphate pathway is the metabolic pathway where NADPH, the reducing force in metabolism, and ribose 5-phosphate, the building block of DNA and RNA, are produced. In this study, the in vitro effects of 5-fluorouracil and Tamoxifen chemotherapeutic agents on glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD), which are key enzymes of the pentose phosphate pathway, were investigated. In the first stage of the study, G6PD and 6PGD enzymes were purified from rat erythrocytes using 2ʹ, 5ʹ-ADP Sepharose-4B affinity chromatography. The control of enzyme activities was determined spectrophotometrically at 340 nm wavelength according to the Beutler method. Then, the in vitro effects of 5-FU and Tamoxifen agents, which are widely used in chemotherapy, on enzyme activities were investigated. The results of the study showed that 5-FU increased the activity of both G6PD and 6PGD enzymes in the concentration range of 0.19-1.9 mM and Tamoxifen in the concentration range of 26-260 µM.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Yusuf Temel ^*
0000-0001-8148-3718
Türkiye

Publication Date

June 30, 2021

Submission Date

October 6, 2020

Acceptance Date

May 27, 2021

Published in Issue

Year 2021 Volume: 42 Number: 2

DOI

https://doi.org/10.17776/csj.806343

IZ

https://izlik.org/JA66WT55FE

Cite

RIS / Bibtex

APA

Temel, Y. (2021). The in vitro effect of 5-FU and Tamoxifen Chemotherapeutics on penthose phosphate pathway enzymes. Cumhuriyet Science Journal, 42(2), 245-251. https://doi.org/10.17776/csj.806343

Cited By

In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme

Türk Doğa ve Fen Dergisi

https://doi.org/10.46810/tdfd.1454573

Öz

The in vitro effect of 5-FU and Tamoxifen Chemotherapeutics on penthose phosphate pathway enzymes

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme