Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction

Ebrar Karaca; Songül Ulusoy; Ümmügülsüm Morgül; Halil İbrahim Ulusoy

doi:10.17776/csj.791813

EN

Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction

Abstract

This study is focused on developing a sensitive analytical method for Streptozotocin (STZ) molecules which is a naturally occurring alkylating antineoplastic agent. It is also known particularly toxic to the insulin-producing beta cells of the pancreas in mammals. With this study, a separation-enrichment method based on solid phase extraction was developed for low levels of STZ. The synthetized material consist of a multi-walled carbon nanotube/CuO/Cu2O was used as an adsorbent in column type SPE. Experimental variables such as pH, ionic strength, desorption solvent, and other parameters were studied and optimized step by step. Analytical merits were obtained under the optimized conditions: linear range (100.00-1500.00 ng mL-1), limit of detection (28.57 ng mL-1), relative standard deviation (RSD) % for 250 ng mL-1 of STZ (< 4.2 %, N:3), and pre-concentration factor (125). In order to test accuracy of the method, recovery tests were applied successively by using model solutions.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Ebrar Karaca
0000-0003-3184-9843
Türkiye

Songül Ulusoy ^*
0000-0003-3640-5416
Türkiye

Ümmügülsüm Morgül
0000-0001-8393-2396
Türkiye

Halil İbrahim Ulusoy
0000-0002-8742-5145
Türkiye

Publication Date

December 29, 2020

Submission Date

September 9, 2020

Acceptance Date

November 11, 2020

Published in Issue

Year 2020 Volume: 41 Number: 4

DOI

https://doi.org/10.17776/csj.791813

IZ

https://izlik.org/JA76PB89DH

Cite

RIS / Bibtex

APA

Karaca, E., Ulusoy, S., Morgül, Ü., & Ulusoy, H. İ. (2020). Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction. Cumhuriyet Science Journal, 41(4), 826-831. https://doi.org/10.17776/csj.791813

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Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction

Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction

Abstract

Keywords

Öz

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

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