The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

Ahmet Kemal Filiz; Sebahattin Karabulut

doi:10.17776/csj.775122

EN

The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

Abstract

Emerging evidence indicates a pathogenic role of protracted neuroinflammation in the various neurodegenerative diseases, including epilepsy. Neuroinflammation may contribute to neuronal hyperexcitability underlying seizure formation. The current research aims to examine the changes in the levels of proinflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the penicillin epilepsy model. In the present study, 12 male Wistar albino rats were randomly divided into two groups as sham and penicillin epilepsy model. Seizures were induced with the intracortical (i.c.) single microinjection 500 IU of penicillin-G into neocortex. Rats were decapitated after observing the cortical epileptic activity and brains were removed by craniotomy. Proinflammatory cytokines (TNF-α and IL-1β) were measured by using ELISA methods in the cortical and hippocampal brain regions. Penicillin significantly up-regulated the expression of IL‑1β and TNF-α in the rat cortex, but did not affect the hippocampal cytokines levels. This study is indicative of the neuroinflammatory potential of cortical penicillin administration.

Keywords

Supporting Institution

Herhangi bir kurumdan destek alınmamıştır.

References

[1] Fisher R.S., Acevedo C., Arzimanoglou A., Bogacz A., Cross J. H., Elger C.E., Engel J., Forsgren L., French J.A., Glynn M., Hesdorffer D.C., Lee B.I., Mathern G.W., Moshé S.L., Perucca E., Scheffer I.E., Tomson T., Watanabe M., Wiebe S., ILAE official report: A Practical Clinical Definition of Epilepsy, Epilepsia, 55(4) (2014) 475-482.
[2] Chen Z., Brodie M.J., Liew D., Kwan P., Treatment Outcomes in Patients with Newly Diagnosed Epilepsy Treated with Established and New Antiepileptic Drugs: A 30-Year Longitudinal Cohort Study, JAMA Neurol., 75(3) (2018) 279-286.
[3] Li Z.H., Li L.L., Jin M.F., Chen X.Q., Sun Q., Ni H., Dysregulation of zinc/lipid metabolism‑associated genes in the rat hippocampus and cerebral cortex in early adulthood following recurrent neonatal seizures, Mol. Med. Rep., 16(4) (2017) 4701-4709.
[4] Vezzani A., French J., Bartfai T., Baram T.Z., The role of inflammation in epilepsy, Nat Rev Neurol., 7(1) (2011) 31-40.
[5] Li G., Bauer S., Nowak M., Norwood B., Tackenberg, B., Rosenow F., Knake S., Oertel W.H., Hamer H.M., Cytokines and Epilepsy. Seizure, 20(3) (2011) 249-256.
[6] Rawat C., Kukal S., Dahiya U.R., Kukreti R., Cyclooxygenase-2 (COX-2) inhibitors: future therapeutic strategies for epilepsy management, J Neuroinflammation, 16(1) (2019) 1-15.
[7] Sumbul O., Aygun H., The effect of vitamin D3 and paricalcitol on penicillin-induced epileptiform activity in rats., Epilepsy Res., 159 (2020) 106262.
[8] Marangoz A.H., Kocacan S.E., Him A., Kuruoglu E., Cokluk C., Marangoz C., Proconvulsant Effect of Papaverine on Penicillin-Induced Epileptiform Activity in Rats., Turk Neurosurg., 28(3) (2018) 479-482.

[9] Bo G.P., Mainardi P., Benassi E., Besio G., Faverio A., Scotto P.A., Loeb C., Parenteral penicillin model of epilepsy in the rat: a reappraisal, Methods Find Exp. Clin. Pharmacol., 8(8) (1986) 491-496.
[10] Paxinos G., Watson C., The Rat Brain in Stereotaxic Coordinates, 6th ed., Amsterdam: Elsevier Academic Press, (2007).
[11] Kruger N.J., The Bradford method for protein quantitation, Methods Mol. Biol., 32 (1994) 9-15.
[12] Webster K.M., Sun M., Crack P., O'Brien T.J., Shultz S.R., Semple B.D., Inflammation in epileptogenesis after traumatic brain injury, J. Neuroinflammation, 14(1) (2017) 10.
[13] Robel S., Buckingham S.C., Boni J.L., Campbell S.L., Danbolt N.C., Riedemann T., Sutor B., Sontheimer H., Reactive astrogliosis causes the development of spontaneous seizures, J Neurosci., 35(8) (2015) 3330-3345.
[14] Eyo U.B., Murugan M., Wu L.J., Microglia-neuron communication in epilepsy, Glia, 65 (2017) 5–18.
[15] Dinarello C.A., Biologic basis for interleukin-1 in disease, Blood, 87(6) (1996) 2095–2147.
[16] De Simoni M.G., Perego C., Ravizza T., Moneta D., Conti M., Marchesi F., De Luigi A., Garattini S., Vezzani A., Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus, Eur. J. Neurosci., 12(7) (2000) 2623-2633.
[17] Wallenstein M.C., Attenuation of penicillin models of epilepsy by nonsteroidal anti-inflammatory drugs, Exp Neurol., 98(1) (1987) 152-160.
[18] Zhu X., Chen Y., Du Y., Wan Q., Xu Y., Wu J., Astragaloside IV attenuates penicillin-induced epilepsy via inhibiting activation of the MAPK signaling pathway, Mol. Med. Rep., 17(1) (2018) 643-647.
[19] Taşkıran AŞ., Taştemur Y., The comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats., Cumhuriyet Sci. J., 41(3) (2020) 635-641.
[20] Devinsky O., Vezzani A., O'Brien T.J., Jette N., Scheffer I.E., de Curtis M., Perucca P., Epilepsy, Nat. Rev. Dis. Primers, 4 (2018) 18024.
[21] Balosso S., Ravizza T., Perego C., Peschon J., Campbell I.L., De Simoni M.G., Vezzani A., Tumor necrosis factor-alpha inhibits seizures in mice via p75 receptors, Ann. Neurol., 57(6) (2005) 804-812.
[22] Shandra A.A., Godlevsky L.S., Vastyanov R.S., Oleinik A.A., Konovalenko V.L., Rapoport E.N., Korobka N.N., The role of TNF-alpha in amygdala kindled rats, Neurosci Res., 42(2) (2002) 147-153.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ahmet Kemal Filiz
0000-0001-9260-5549
Türkiye

Sebahattin Karabulut ^*
0000-0002-3261-4125
Türkiye

Publication Date

March 29, 2021

Submission Date

July 28, 2020

Acceptance Date

March 6, 2021

Published in Issue

Year 2021 Volume: 42 Number: 1

DOI

https://doi.org/10.17776/csj.775122

IZ

https://izlik.org/JA32DW69UK

Cite

RIS / Bibtex

APA

Filiz, A. K., & Karabulut, S. (2021). The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal, 42(1), 1-6. https://doi.org/10.17776/csj.775122