Biological Activity of Some Pyrimidine Derivatives: Cytotoxicity and Oxidative Stress Potential in Human Lung Cancer Cell line (A549).

Mahmoud Abudayyak; Fatma Betül Şamlıoğlu; Beyza Selen; Seda Fandaklı; Nurettin Yaylı

doi:10.17776/csj.758957

EN

Biological Activity of Some Pyrimidine Derivatives: Cytotoxicity and Oxidative Stress Potential in Human Lung Cancer Cell line (A549).

Abstract

Compounds with pyrimidine ring in their structure have many biological activities including antimicrobial, antiviral and anticancer. Recently, studies related to their synthesis and so their applications have increased. In a previous study, a solid-phase microwave method was used to synthesized 25 new hydroxy- and methoxy-substituent 4,6-diarylpyrimidin-2 (1H) -ol and 4,6 diarylpyrimidin-2 (1H) –thiol compounds. In the present study, as a preliminary estimation of the anticancer activity, the cytotoxicity and oxidative stress induction potential of 6 derivatives that show highest antibacterial activities was evaluated in human lung epithelial cancer cell line (A549). Results of the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity test indicate that pyrimidine derivatives caused concentration-dependent cell death that IC50 values were calculated between 16.7 and 41.5 µg/mL. additonally, pyrimidine derivatives induced significant changes in malondialdehyde (MDA) and glutathione (GSH) levels and catalase (CAT) activity; oxidative stress could be the mechanism of action of the tested pyrimidine derivatives in the cancerous cells. The results could be used to design the further in vivo and in vitro detailed studies to appreciate these pyrimidine derivatives anticancer activity, compare this activity with in-use known drugs, elucidate their mechanisms of action and estimate their safety.

Keywords

Supporting Institution

TÜBİTAK (2209-A)

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Mahmoud Abudayyak ^*
Türkiye

Fatma Betül Şamlıoğlu
0000-0001-5940-2512
Türkiye

Beyza Selen
0000-0003-2022-2033
Türkiye

Seda Fandaklı
0000-0002-8199-3336
Türkiye

Nurettin Yaylı
0000-0003-4174-3014
Türkiye

Publication Date

December 29, 2020

Submission Date

June 27, 2020

Acceptance Date

December 6, 2020

Published in Issue

Year 2020 Volume: 41 Number: 4

DOI

https://doi.org/10.17776/csj.758957

IZ

https://izlik.org/JA25RA22YF

Cite

RIS / Bibtex

APA

Abudayyak, M., Şamlıoğlu, F. B., Selen, B., Fandaklı, S., & Yaylı, N. (2020). Biological Activity of Some Pyrimidine Derivatives: Cytotoxicity and Oxidative Stress Potential in Human Lung Cancer Cell line (A549). Cumhuriyet Science Journal, 41(4), 756-763. https://doi.org/10.17776/csj.758957

Öz

Biological Activity of Some Pyrimidine Derivatives: Cytotoxicity and Oxidative Stress Potential in Human Lung Cancer Cell line (A549).

Abstract

Keywords

Supporting Institution

References

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Published in Issue

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