Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231

İdil Çetin

doi:10.17776/csj.751778

EN

Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231

Abstract

In this study, antiproliferative effects of the anti-EGFR monoclonal antibody Cetuximab were evaluated using MDA-MB-231 cell line originated from triple negative breast cancer. As cell kinetic parameters, we evaluated Cell index, mitotic index, labeling index and apoptotic index. For this purpose, 20 μM, 45 μM and 60 μM Cetuximab concentrations were applied to the cells using the real-time cell analysis system (xCelligence DP) and IC50 values were determined. IC50 concentrations were used for all other parameters. According to experimental results, Cetuximab administration inhibited cell kinetics of MDA-MB-231 cells. xCelligence DP instrument detected IC50 concentrations of Cetuximab for cell line. These values were 45 μM for MDA-MB-231 cells. When these IC50 value applied to cells, significant decrease was detected in mitotic index, labelling index and significant increase was detected in apoptotic index for experimental groups. Student’s t tests for paired samples were used to assign statistical significance. p<0.05 level of significance was accepted. According to the results obtained, Cetuximab has the potential to slow down the prognosis of the triple negative breast cancer subtype.

Keywords

Supporting Institution

Scientific Research Project Coordination Unit of Istanbul University

Project Number

FBA-2017-24288

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

İdil Çetin ^*
0000-0002-3961-6422
Türkiye

Publication Date

September 30, 2020

Submission Date

June 12, 2020

Acceptance Date

September 1, 2020

Published in Issue

Year 2020 Volume: 41 Number: 3

DOI

https://doi.org/10.17776/csj.751778

IZ

https://izlik.org/JA84MN37HD

Cite

RIS / Bibtex

APA

Çetin, İ. (2020). Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231. Cumhuriyet Science Journal, 41(3), 706-711. https://doi.org/10.17776/csj.751778

AMA

1.Çetin İ. Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231. CSJ. 2020;41(3):706-711. doi:10.17776/csj.751778

Chicago

Çetin, İdil. 2020. “Antiproliferative Effects of Cetuximab on Triple Negative Breast Cancer Cell Line MDA-MB-231”. Cumhuriyet Science Journal 41 (3): 706-11. https://doi.org/10.17776/csj.751778.

EndNote

Çetin İ (September 1, 2020) Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231. Cumhuriyet Science Journal 41 3 706–711.

IEEE

[1]İ. Çetin, “Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231”, CSJ, vol. 41, no. 3, pp. 706–711, Sept. 2020, doi: 10.17776/csj.751778.

ISNAD

Çetin, İdil. “Antiproliferative Effects of Cetuximab on Triple Negative Breast Cancer Cell Line MDA-MB-231”. Cumhuriyet Science Journal 41/3 (September 1, 2020): 706-711. https://doi.org/10.17776/csj.751778.

JAMA

1.Çetin İ. Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231. CSJ. 2020;41:706–711.

MLA

Çetin, İdil. “Antiproliferative Effects of Cetuximab on Triple Negative Breast Cancer Cell Line MDA-MB-231”. Cumhuriyet Science Journal, vol. 41, no. 3, Sept. 2020, pp. 706-11, doi:10.17776/csj.751778.

Vancouver

1.İdil Çetin. Antiproliferative effects of cetuximab on triple negative breast cancer cell line MDA-MB-231. CSJ. 2020 Sep. 1;41(3):706-11. doi:10.17776/csj.751778