Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection

Fatos Ayca Ozdemir Olgun; Birsen Demirata

doi:10.17776/csj.723599

EN

Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection

Abstract

Penicillin G, tetracycline and oxytetracycline are broad-spectrum antibiotics with considerably low side effects which are used in most of the poultries. Due to complex matrix effects, it is considerably difficult to isolate these compounds from other molecules (such as proteins and lipids) in order to analyze. In this study, a high performance liquid chromatography (HPLC) coupled with photodiode array (PDA) detector system was introduced for the determination of tetracycline, oxytetracycline and penicillin G residues in nine chicken breast samples. For the separation of tetracycline and oxytetracycline out of samples prior to the analysis, trichloroacetic acid (TCA) and McIlvaine buffer solutions were used while formic acid and phosphate buffer solutions were utilized for penicillin G using a solid phase extraction system. The peaks at the chromatograms were enlightened and recovery percentages were calculated using spiked samples (96.57% for penicillin G; 99.00% for oxytetracycline; 95.92% for tetracycline). LOD and LOQ values were calculated, respectively as 1.55 gL-1 and 5.20 gL-1 for tetracycline; 1.32 gL-1 and 4.39 gL-1 for oxytetracycline; 1.07 gL-1 and 3.60 gL-1 for penicillin G. With the proposed method, the determination of three antibiotic residues was performed in less than 15 minutes and applied successfully to real samples.

Keywords

References

Inal T., Besin hijyeni, hayvansal gıdaların sağlık kontrolu, Final Ofset, (1992) 55-57.
Jayalakshmi K., Paramasivam M., Sasikala M., Tamilam T. V., Sumiho A., J. Entomol. Zool. Stud., 5 (2017) 1446-1451.
Mitchel J., Griffiths M. W., Mceven W. B., Mcnab A. J., Yee A., Antimicrobial drug residues in milk and meat; causes, concerns, prevalence, regulations, tests and test performance, J Food Protect 61(6) (1988) 742-756.
EC (1990) Council Directive 90/167/EEC of 26 March 1990 laying down the conditions governing the preparation, placing on the market and use of medicated feeding stuffs in the Community, Council Directive 90/167/EEC (1990). Offic J Eur Comm L92:42–48
Berrada H., Molto J.C., Manes J., Font G., Determination of Aminoglycoside and Macrolide Antibiotics in Meat by Pressurized Liquid Extraction and LC-ESI-MS., J Sep Sci., 33 (2010) 522–529.
Percin Ozkorucuklu S., Uka B., Yildirim Bastemur G., Voltammetric Analysis of Cephalexin and Cefazolin in Pharmaceutical Formulations and Biological Samples., JOTSCA, 6(2) (2019) 217-224.
Young-Song J., Jung Hu S., Joo H., Hwang J. B., Kim M. O., Shin J. K., Cho D., Determination of Penicillin Residues in Livestock and Marine Products by LC/MS/MS, WASET, 81 (2011) 809-811.
Granelli K., Brazzell C., Rapid multi-residue screening of antibiotics in muscle and kidney by liquid chromatography-electrospray ionization–tandem mass spectrometry, J.ACA., 585 (2007) 289-295.

Furusawa N., Simplified liquid-chromatographic determination of residues of tetracycline antibiotics in eggs, Chromatographia, 53 (2001) 47-50.
Schneider M. J., Braden S. E., Herrera I. R., Donoghue D. J., Simultaneous determination of fluoroquinolones and tetracyclines in chicken muscle using HPLC with fluorescence detection, J Chromatography B, 846 (2007) 8-13.
Lu H., Jiang Y., Li H., Chen F., Wong M., Simultaneous determination of oxytetra-cycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection, Chromatographia, 60(5-6), 259-264.
Cháfer-Pericás C., Maquieira A., Puchades R., Fast screening methods to detect antibiotic residues in food samples, TrAC, 29 (9) (2010) 1038-1049.
Yuvalı D., Narin İ., Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc, Cumhuriyet Sci. J., 41(1) (2020) 239-244.
Bousova K., Senyuva H., Mitterdorf K., Quantitative multi-residue method for determination antibiotics in chicken meat using turbulent flow chromatography coupled to liquid-chromatography-tandem mass spectrometry, J. Chrom. A, 1274 (2013) 19- 27.
Tong J., Rao Q., Zhu Z., Jiang Z., Ding S., Simultaneous determination of five tetracycline and macrolide antibiotics in feeds using HPCE, J. Sep. Sci., 32 (23-24) (2009) 4254–4260.
Aszalos A., Fast determination of tetracycline antibiotics in different fast determination of tetracycline antibiotics in different media by High-Performance Liquid Chromatography. Chromatographia, 20 (1985) 313-322.
Shalaby A. R., Salama N. A., Abouraya S. H., Mehaya F. M., Incidence of tetracycline residues in chicken meat and liver retailed to consumers, Food Chem., 124 (2011) 1660-1666.
Zief M., Solid Phase Extraction for Sample Preparation, Phillipsburg: JT Baker (2005).
World Health Organization, 70th report of the Joint FAO/ WHO expert comittee on food additives, Geneva, 21-29 October (2008).
Magon T., Silveira R., Galuch M. B., Fagan E. P., Feitoza F. D., Palombini S. V., Santos O. O., Visenteiner J. V., Simultaneous determination of four antibiotics in raw milk by UPLC-MS/MS using protein precipitation as sample preparation: Development, validation, and application in real samples, J. Braz. Chem. Soc., 29 (2018) 2441-2448.
Cetinkaya F., Yibar A., Soyutemiz G. E., Okutan B., Ozcan A., Karaca M. Y., Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry, Food Addit. Contann. Part B Surveill, 5 (2012) 9-45.
Kowalski P., Koniecznna L., Determination of penicillin antibiotics in poultry muscle by capillary electrophoresis, Bull Vet Inst Pulawy, 51 (2007) 595-598.
Jammoul A., Darra N., Evaluation of antibiotic residues in chicken meat samples in Lebanon antibiotics, Basel , 8 (2019) 69.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Fatos Ayca Ozdemir Olgun ^*
0000-0003-1077-2621
Türkiye

Birsen Demirata
0000-0002-0978-0977
Türkiye

Publication Date

September 30, 2020

Submission Date

April 20, 2020

Acceptance Date

August 4, 2020

Published in Issue

Year 2020 Volume: 41 Number: 3

DOI

https://doi.org/10.17776/csj.723599

IZ

https://izlik.org/JA98YT52FH

Cite

RIS / Bibtex

APA

Ozdemir Olgun, F. A., & Demirata, B. (2020). Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection. Cumhuriyet Science Journal, 41(3), 665-670. https://doi.org/10.17776/csj.723599

AMA

1.Ozdemir Olgun FA, Demirata B. Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection. CSJ. 2020;41(3):665-670. doi:10.17776/csj.723599

Chicago

Ozdemir Olgun, Fatos Ayca, and Birsen Demirata. 2020. “Evaluation of Tetracycline, Oxytetracycline and Penicillin G Residues in Chicken Meat Samples by High Performance Liquid Chromatography With Pda Detection”. Cumhuriyet Science Journal 41 (3): 665-70. https://doi.org/10.17776/csj.723599.

EndNote

Ozdemir Olgun FA, Demirata B (September 1, 2020) Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection. Cumhuriyet Science Journal 41 3 665–670.

IEEE

[1]F. A. Ozdemir Olgun and B. Demirata, “Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection”, CSJ, vol. 41, no. 3, pp. 665–670, Sept. 2020, doi: 10.17776/csj.723599.

ISNAD

Ozdemir Olgun, Fatos Ayca - Demirata, Birsen. “Evaluation of Tetracycline, Oxytetracycline and Penicillin G Residues in Chicken Meat Samples by High Performance Liquid Chromatography With Pda Detection”. Cumhuriyet Science Journal 41/3 (September 1, 2020): 665-670. https://doi.org/10.17776/csj.723599.

JAMA

1.Ozdemir Olgun FA, Demirata B. Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection. CSJ. 2020;41:665–670.

MLA

Ozdemir Olgun, Fatos Ayca, and Birsen Demirata. “Evaluation of Tetracycline, Oxytetracycline and Penicillin G Residues in Chicken Meat Samples by High Performance Liquid Chromatography With Pda Detection”. Cumhuriyet Science Journal, vol. 41, no. 3, Sept. 2020, pp. 665-70, doi:10.17776/csj.723599.

Vancouver

1.Fatos Ayca Ozdemir Olgun, Birsen Demirata. Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection. CSJ. 2020 Sep. 1;41(3):665-70. doi:10.17776/csj.723599

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