Synthesis of polyhedral oligomeric silsesquioxane-n-acetylcysteine conjugate with click chemistry and ıts antioxidant response and biocompatibility

İdil Karaca Açarı; Sevgi Balcıoğlu; Burhan Ateş; Süleyman Köytepe; İsmet Yılmaz; Turgay Seçkin

doi:10.17776/csj.688124

EN

Synthesis of polyhedral oligomeric silsesquioxane-n-acetylcysteine conjugate with click chemistry and ıts antioxidant response and biocompatibility

Abstract

In this study, polyhedral oligomeric silsesquioxane-N-acetylcysteine (POSS-NAC) conjugate as a potential antioxidant molecule was synthesized from N-acetylcysteine (NAC) and amino-functional POSS structure by click chemistry. The chemical structures and thermal properties of the synthesised POSS-NAC conjugate was characterized by spectroscopic and thermal analysis techniques. The antioxidant capacity of the POSS-NAC conjugate was also determined by the 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and reducing power methods. According to the reducing power method, POSS-NAC structure has lower reducing activity than standard ascorbic acid and trolox (p<0.001). It was found from the ABTS radical scavenging activity results that the synthesized POSS-NAC conjugate had a significantly higher radical scavenging effect than the standards (p <0.001). Biocompatibility properties of the POSS-NAC structure were detected in vitro cell culture system with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test on L-929 mouse fibroblast cells. The synthesized POSS-NAC conjugate exhibits high antioxidant activity and good biocompatibility.

Keywords

Polyhedral oligomeric silsesquioxane (POSS),,N-Acetylcysteine (NAC),,antioxidant activity,,biocompatibility

Supporting Institution

Inonu University‘s scientific research projects unit (BAP)

Project Number

2013/157

Thanks

Inonu University‘s scientific research projects unit (BAP)

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

İdil Karaca Açarı ^*
0000-0001-6783-7030
Türkiye

Sevgi Balcıoğlu
0000-0003-0724-4772
Türkiye

Burhan Ateş
0000-0001-6080-229X
Türkiye

Süleyman Köytepe
0000-0002-4788-278X
Türkiye

İsmet Yılmaz
0000-0002-7204-1050
Türkiye

Turgay Seçkin
0000-0001-8483-7366
Türkiye

Publication Date

June 25, 2020

Submission Date

February 11, 2020

Acceptance Date

May 7, 2020

Published in Issue

Year 2020 Volume: 41 Number: 2

DOI

https://doi.org/10.17776/csj.688124

IZ

https://izlik.org/JA55ZS53NB

Cite

RIS / Bibtex

APA

Karaca Açarı, İ., Balcıoğlu, S., Ateş, B., Köytepe, S., Yılmaz, İ., & Seçkin, T. (2020). Synthesis of polyhedral oligomeric silsesquioxane-n-acetylcysteine conjugate with click chemistry and ıts antioxidant response and biocompatibility. Cumhuriyet Science Journal, 41(2), 386-396. https://doi.org/10.17776/csj.688124

Öz

Synthesis of polyhedral oligomeric silsesquioxane-n-acetylcysteine conjugate with click chemistry and ıts antioxidant response and biocompatibility

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite