Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc

Dönay Yuvalı; İbrahim Narin

doi:10.17776/csj.658063

EN

Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc

Abstract

In this study, a simple, sensitive, precise, rapid and reversed-phase high performance liquid chromatographic method with ultraviolet detection for simultaneous determination of Sulfachloropyridazine and Trimethoprim is developed and applied commercial veterinary formulation for chromatographic separation, Thermoscientific Hypersil-C18, reverse phase column was used. Separation was done using acetonitrile: pH 3.0 buffer solution (30:70, v/v) at 0.8 ml min-1 flow rate. Analysis was done 272.0 nm at ultraviyolet detector. Under optimum chromatographic conditions, retention time of Sulfachloropyridazine and Trimethoprim were determined as 4.37 and 2.60 minute. The proposed method was linear in the range 1.0-100.0 μg ml-1 for Sulfachloropyridazine and 0.5-40.0 μg ml-1 for Trimethoprim. The Limit of detection of Sulfachloropyridazine and Trimethoprim are 0.05 μg ml-1, 0.18 μg ml-1, respectively. The method which is rapid, simple and does not require any separation step, has been successfully applied to assay of commercial dosage forms containing Sulfachloropyridazine and Trimethoprim.

Keywords

Determination,HPLC,Sulfachloropyridazine,Veterinary formulation,Trimethoprim

References

[1] Plumb D.C, Veterinary Drug Handbook (3rd ed.), Iowa State University Press, Ames, (1999) 584-587.
[2] Kanbur M., Liman C., Eraslan G., et al. Effects of Various Cooking and Freezing Processes on the Residues of Sulfachlorpyridazine-Trimethoprim Combination in Broiler Tissues. Kafkas Univ. Vet Fak Derg , 20 (4) (2010) 487-492.
[3] Agrawal K. V., Analysis of Antibiotic/Drug Residues in Food Products of Animal origin, (1st ed), Plenum Press, New York, (1991) 11-140.
[4] Hess M. M., Boucher B.A., Laizure S. C., et al. Trimethoprim-sulfamethoxazole pharmacokinetics in trauma patients. Pharmacotherapy. 13(6) (1993) 602-606.
[5] Stephen H., Kenneth H., in Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (Eighth Edition), Elsevier Inc. (2015).
[6] Zhang W., Duan C., Wang M., Analysis of seven sulphonamides in milk by cloud point extraction and high performance liquid chromatography. Food Chemistry, 126 (2011) 779-785.
[7] Blackwell A.P, Holten Lützhøft C.H, Mab P.H. et al. Fast and robust simultaneous determination of three veterinary antibiotics in groundwater and surface water using a tandem solid-phase extraction with high-performance liquid chromatography–UV detection. J Chromatography A, 1045 (2004) 111–117.
[8] Kristof E. M, Zhang G.F, Lambert W.E. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. J Chromatography A, 1047 (2004) 85–92.

[9] Amini H., Ahmadiani A., Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography. J Pharmaceutical and Biomedical Analysis, 43 (2007) 1146–1150.
[10] Pereira A.V, Cass Q.B, High-performance liquid chromatography method for the simultaneous determination of sulfamethoxazole and trimethoprim in bovine milk using an on-line clean-up column. J Chromatography B, 826 (2005) 139–146.
[11] Kanbur M., Narin İ., Özdemir E., Fractional Wavelet Transform for the Quantitative Spectral Analysis of Two-Component System. Fractional Modelling, In: New Trends in Nanotechnology and Fractional Calculus Applications, Baleanu D., Güvenç Z.B., Machado T.J.A., Springer Dordrecht Heidelberg, London, New York, (2010) 321-331.
[12] Granero G., Garnero C., Longhi M., Second derivative spectrophotometric determination of trimethoprime and sulfamethoxazole in the presence of hydroxypropyl-β-cyclodextrin. J Pharmaceutical and Biomedical Analysis, 29 (2002) 51–59.
[13] Gallego J. M, Arroyo J., Spectrophotometric resolution of ternary mixtures of Dexamethasone, Polymyxin B and Trimethoprim in synthetic and pharmaceutical formulations. Analytica Chimica Acta, 437 (2001) 247–257.
[14] López-Martı́nez L., López-de-Alba P.L., de-León-Rodrı́guez L.M., Yepez-Murrieta M.L., Simultaneous determination of binary mixtures of trimethoprim and sulfamethoxazole or sulphamethoxypyridazine by the bivariate calibration spectrophotometric method. Journal of Pharmaceutical and Biomedical Analysis 30(1) (2002) 77-85.
[15] Le-Minh N., Stuetz M. R., Khan S.J., Determination of six sulfonamide antibiotics, two metabolites and trimethoprim in wastewater by isotope dilution liquid chromatography/tandem mass spectrometry, Talanta 89 (2012) 407– 416.
[16] Economou A., Petraki O., Tsipi D., Botitsi E.. A liquid chromatography–tandem mass spectrometry method for the determination of sulfonamides, trimethoprim and dapsone in honey and validation according to Commission Decision 2002/657/EC for banned compounds. Talanta, 97 (2002) 32-41.
[17] Solliec M., Masse D., Sauve S., Analysis of trimethoprim, lincomycin, sulfadoxin and tylosin in swine manure using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem mass spectrometry. Talanta, 128 (2014) 23-30.
[18] Forti A.F, Scortichini G., Determination of ten sulphonamides in egg by liquid chromatography–tandem mass spectrometry, Analytica Chimica Acta 637 (2009) 214–219.
[19] Lara F.J, García-Campa˜na M.A, Neusüss C., Alés-Barrero F., Determination of sulfonamide residues in water samples by in-line solid-phase extraction-capillary electrophoresis, J of Chromatography A, 1216 (2009) 3372–3379.
[20] da Silva I.S., Vidal D.T., do Lago C.L., Angnes L., Fast simultaneous determination of trimethoprim and sulfamethoxazole by capillary zone electrophoresis with capacitively coupled contactless conductivity detection. J Sep Sci. 36(8) (2013) 1405-1409.
[21] Ni Y., Qi Z., Kokot S., Simultaneous ultraviolet–spectrophotometric determination of sulfonamides by multivariate calibration approaches. Chemometrics and Intelligent Laboratory Systems. 82 (1-2) (2006) 241-247.
[22] Pokala R. V., Kusuma Kumari K., Bollikola H. B., UV spectrophotometry method development and validation of sulfadiazine and trimethoprim in combined dosage form. Int J Pharm Pharm Sci. 10(1) (2018) 103-107.
[23] Abd-Alrassol K.S., Spectrophotometric method for the determination of sulfa drug in pharmaceuticals based on charge transfer reaction. J. of Chemical and Pharmaceutical Research. 9(2) (2017) 244-251.
[24] Tahan G. P., Machado S.C., Malaguti E. C., Maia P.P., Susanne Rath S., Martins I., RP-LC method for simultaneous determination ofsulfamethoxazole and trimethoprim content in veterinary drugs . Eclética Química. 40 (2015) 32-41.
[25] Goulas V., Anisimova Andreou T., Angastinioti Moditi C., Tzamaloukas O., A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes. Journal of Animal and Feed Science. 23 (2014) 185–189.
[26] The European Agency for the evaluation of Medicinal Products. ICH Topic Q2B Notefor Guideline on Validation of Analytical procedures: Methodology GPMP/ICH/281/95, 1996 Retrieved from; https://www.ema.europa.eu/en/documents/scientific-guideline/ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Dönay Yuvalı ^*
0000-0001-9407-8826
Türkiye

İbrahim Narin
Türkiye

Publication Date

March 22, 2020

Submission Date

December 11, 2019

Acceptance Date

February 19, 2020

Published in Issue

Year 2020 Volume: 41 Number: 1

DOI

https://doi.org/10.17776/csj.658063

IZ

https://izlik.org/JA62MT67XL

Cite

RIS / Bibtex

APA

Yuvalı, D., & Narin, İ. (2020). Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc. Cumhuriyet Science Journal, 41(1), 239-244. https://doi.org/10.17776/csj.658063