Research Article
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Year 2020, Volume: 41 Issue: 1, 228 - 234, 22.03.2020

Meltem Aşan Özüsağlam , Ayşe Günyaktı

https://doi.org/10.17776/csj.654353
https://izlik.org/JA83JS72LG

Abstract

References

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  • [2] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Xaus J., Fernández L. and Rodríguez J.M., Human milk is a source of lactic acid bacteria for the infant gut, Journal of Pediatrics, 143 (2003) 754-758.
  • [3] Jeurink P.V., van Bergenhenegouwen J., Jimenez E., Knippels L.M, Fernandez L., Garssen J., Knol J., Rodriguez J.M. and Martin R., Human milk: a source of more life than we imagine, Benef. Microbes, 4 (2013) 17–30.
  • [4] Fitzstevens J.L., Smith K.C., Hagadorn J.I.,Caimano M.J., Matson A.P. and Brownell E.A., Systematic review of the human milk microbiota, Nutr. Clin. Pract., 32 (2017) 354–364.
  • [5] Perez P.F., Dore J., Leclerc M., Levenez F., Benyacoub J., Serrant P., Segura-Roggero I, Schiffrin E.J. and Donnet-Hughes A., Bacterial imprinting of the neonatal immune system: lessons from maternal cells?, Pediatrics, 119 (2007) e724–e732.
  • [6] Chiu Y.-H., Tsai J.-J., Lin S.-L., Choritosvakin C. and Lin M.-Y., Characterisation of bifidobacteria with immunomodulatory properties isolated from human breast milk, J. Funct. Foods, 7 (2014) 700–708.
  • [7] Moles L., Escribano E., Andrés, J. De Montes M.T., Rodríguez J.M., Jiménez E., Sáenz de Pipaón M. and Espinosa-Martos I., Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study, J. Immunol. Res., (2015) 538171. doi: 10.1155/2015/538171
  • [8] Shin S.P., Choi Y.M., Kim WH., Hong S.P., Park J.M., Kim J., Kwon O., Lee E.H. and Hahm K.B., A double blind, placebo-controlled, randomized clinical trial that breast milk derived-Lactobacillus gasseri BNR17 mitigated diarrhea-dominant irritable bowel syndrome, J. Clin. Biochem. Nutr., 62 (2018) 179–186.
  • [9] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Olivares M., Boza J., Jiménez J., Fernandez L. and Xaus J., The comensal microflora of human milk: new perspectives for food bacteriotherapy and probiotics, Trends Food Sci. Technol., 15 (2004) 121–127.
  • [10] Morelli L. and Capurso L., FAO/WHO guidelines on probiotics 10 years latter, J. Clin. Gastroenterol., 46 (2012) 1–2.
  • [11] Barbosa F.H.F., Barbosa L.P.J.L., Bambirra L.H.S. and Aburjaile F.F., Probioticos – microrganismos a favor da vida, Rev. Biol. Cien. Terra, 11 (2011) 11–21.
  • [12] FAO/WHO, Guidelines for the Evaluation of Probiotics in Food, Food and Agriculture Organization (FAO), World Health Organization (WHO), Geneva, Switzerland, 2002.
  • [13] Gupta V. and Garg R., Probiotics, Indian J. Med. Microbiol., 27 (2009) 202–209.
  • [14] FAO/WHO, Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food London, Ontario, Canada; April 30 and May 1, 2002.
  • [15] Gunyakti A. and Asan-Ozusaglam M., Lactobacillus gasserifrom human milk with probiotic potential and some technological properties, LWT – Food Science and Technology, 109 (2019) 261-269.
  • [16] Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty- Second Informational Supplement. CLSI Document M100-S22, Clinical Laboratory Standard Institute, Wayne, PA. ISBN 1-56238-785-5, (Print); ISBN 1-56238-786-3 (2012) (Electronic)
  • [17] Zoral S., İnsan kaynaklı Lactobacillus spp. Suşlarının probiyotik özelliklerinin belirlenmesi. MSc thesis, University of Ahi Evran, Kırşehir, Turkey, 2013.
  • [18] Tokatlı M., Identification of lactic acid bacteria isolated from pickles in Ankara Çubuk region, determination of their technological and functional properties and their potential for using as a starter culture, PhD thesis, University of Ankara, Ankara, Turkey, 2013.
  • [19] Yürümez E., Probiotics properties of some lactic acid bacteria isolated from faeces samples. MSc thesis, University of Ankara, Ankara, Turkey, 2011.
  • [20] Xu H., Jeong H.S., Lee H.Y. and Ahn J., Assessment of cell surface properties and adhesion potential of selected probiotic strains. Lett. Appl. Microbiol., 49 (2009) 434–442.
  • [21] Georgieva R., Yocheva L., Tserovska L., Zhelezova G., Stefanova N., Atanasova A., Danguleva A., Ivanova G., Karapetkov N., Rumyan N. and Karaivanova E., Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures, Biotechnol. Biotechnol. Equip., 29 (2015) 84–91.
  • [22] Zheng M., Zhang R., Tian X., Zhou X., Pan X. and Wong A., Assessing the risk of probiotic dietary supplements in the context of antibiotic resistance, Front. Microbiol., 8 (2017) 908. doi: 10.3389/fmicb.2017.00908
  • [23] Danielsen M. and Wind A., Susceptibility of Lactobacillus spp. to antimicrobial agents, Int. J. Food Microbiol., 82 (2003) 1-11.
  • [24] Berardi C. W., Solovey E.T. and Cummings M.L., Investigating the efficacy of network visualizations for intelligence tasks, in Proceedings of the IEEE International Conference on Intelligence and Security Informatics (Seattle, WA), (2013) 278–283.
  • [25] Plessas S., Nouska C., Karapetsas A., Kazakos S., Alexopoulos A., Mantzourani I., Chondrou P., Fournomiti M., Galanis A. and Bezirtzoglou E., Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese, Food Chem., 226 (2017) 102–108.
  • [26] Singroha G., Mishra S.K. and Malik R.K., Isolation and characterization of potential probiotic Lactobacillus gasseri strains isolated from different sources, Int. J. Food. Ferment., 6 (2017) 71-83.
  • [27] Oh N.S., Joung J.Y., Lee J.Y. and Kim Y., Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces. PLoSONE, 13(2) (2018) e0192021. https://doi.org/10.1371/journal.pone.0192021
  • [28] Argyri A.A.,Zoumpopoulou G., Karatzas K.A., Tsakalidou E., Nychas G.J., Panagou E.Z. and Tassou C.C., Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests, Food Microbiol., 33 (2013) 282-291.
  • [29] Ferreira C.L., Grześkowiak L., Collado M.C. and Salminen S., In vitro evaluation of Lactobacillus gasseri strains of infant origin on adhesion and aggregation of specific pathogens, J. Food Prot., 74 (2011) 1482–1487.
  • [30] Twetman L., Larsen U., Fiehn N.-E., Steckse ́n-Blicks C. and Twetman S., Coaggregation between probiotic bacteria and caries-associated strains: An in vitro study, Acta Odontol. Scand., 67 (2009) 284-288.
  • [31] Ocaña V.S. and Nader-Macias M.E., Vaginal lactobacilli: Self- and co-aggregating ability, Br. J. Biomed. Sci., 59 (2002) 183–190.

Investigation of lactobacillus gasseri ma-2 as probiotic candidate

Year 2020, Volume: 41 Issue: 1, 228 - 234, 22.03.2020

Meltem Aşan Özüsağlam , Ayşe Günyaktı

https://doi.org/10.17776/csj.654353
https://izlik.org/JA83JS72LG

Abstract

Characterization of lactic acid bacteria in human milk has become an interesting field for developing new probiotics. Therefore, some probiotic properties of Lactobacillus gasseri MA-2 strain isolated before from human breast milk were screened in vitro. MA-2 strain exhibited safety aspects with no hemolytic activity (γ-hemolytic activity) and sensitivity to penicillin G, cloxacillin and chloramphenicol. The remarkable tolerance to various acid, bile, pepsin and pancreatin conditions was determined with high survival rate. The strain also presented significant auto-aggregation (93%) as well as co-aggregation activity with pathogenic microorganisms. L. gasseri MA-2 originated from human milk exhibited promising probiotic properties.

Keywords

probiotic human milk survival gastrointestinal condition aggregation

References

  • [1] Dai D. and Walker W. A., Protective nutrients and bacterial colonization in the immature human gut, Advances in Pediatrics, 46 (1999) 353–382.
  • [2] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Xaus J., Fernández L. and Rodríguez J.M., Human milk is a source of lactic acid bacteria for the infant gut, Journal of Pediatrics, 143 (2003) 754-758.
  • [3] Jeurink P.V., van Bergenhenegouwen J., Jimenez E., Knippels L.M, Fernandez L., Garssen J., Knol J., Rodriguez J.M. and Martin R., Human milk: a source of more life than we imagine, Benef. Microbes, 4 (2013) 17–30.
  • [4] Fitzstevens J.L., Smith K.C., Hagadorn J.I.,Caimano M.J., Matson A.P. and Brownell E.A., Systematic review of the human milk microbiota, Nutr. Clin. Pract., 32 (2017) 354–364.
  • [5] Perez P.F., Dore J., Leclerc M., Levenez F., Benyacoub J., Serrant P., Segura-Roggero I, Schiffrin E.J. and Donnet-Hughes A., Bacterial imprinting of the neonatal immune system: lessons from maternal cells?, Pediatrics, 119 (2007) e724–e732.
  • [6] Chiu Y.-H., Tsai J.-J., Lin S.-L., Choritosvakin C. and Lin M.-Y., Characterisation of bifidobacteria with immunomodulatory properties isolated from human breast milk, J. Funct. Foods, 7 (2014) 700–708.
  • [7] Moles L., Escribano E., Andrés, J. De Montes M.T., Rodríguez J.M., Jiménez E., Sáenz de Pipaón M. and Espinosa-Martos I., Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study, J. Immunol. Res., (2015) 538171. doi: 10.1155/2015/538171
  • [8] Shin S.P., Choi Y.M., Kim WH., Hong S.P., Park J.M., Kim J., Kwon O., Lee E.H. and Hahm K.B., A double blind, placebo-controlled, randomized clinical trial that breast milk derived-Lactobacillus gasseri BNR17 mitigated diarrhea-dominant irritable bowel syndrome, J. Clin. Biochem. Nutr., 62 (2018) 179–186.
  • [9] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Olivares M., Boza J., Jiménez J., Fernandez L. and Xaus J., The comensal microflora of human milk: new perspectives for food bacteriotherapy and probiotics, Trends Food Sci. Technol., 15 (2004) 121–127.
  • [10] Morelli L. and Capurso L., FAO/WHO guidelines on probiotics 10 years latter, J. Clin. Gastroenterol., 46 (2012) 1–2.
  • [11] Barbosa F.H.F., Barbosa L.P.J.L., Bambirra L.H.S. and Aburjaile F.F., Probioticos – microrganismos a favor da vida, Rev. Biol. Cien. Terra, 11 (2011) 11–21.
  • [12] FAO/WHO, Guidelines for the Evaluation of Probiotics in Food, Food and Agriculture Organization (FAO), World Health Organization (WHO), Geneva, Switzerland, 2002.
  • [13] Gupta V. and Garg R., Probiotics, Indian J. Med. Microbiol., 27 (2009) 202–209.
  • [14] FAO/WHO, Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food London, Ontario, Canada; April 30 and May 1, 2002.
  • [15] Gunyakti A. and Asan-Ozusaglam M., Lactobacillus gasserifrom human milk with probiotic potential and some technological properties, LWT – Food Science and Technology, 109 (2019) 261-269.
  • [16] Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty- Second Informational Supplement. CLSI Document M100-S22, Clinical Laboratory Standard Institute, Wayne, PA. ISBN 1-56238-785-5, (Print); ISBN 1-56238-786-3 (2012) (Electronic)
  • [17] Zoral S., İnsan kaynaklı Lactobacillus spp. Suşlarının probiyotik özelliklerinin belirlenmesi. MSc thesis, University of Ahi Evran, Kırşehir, Turkey, 2013.
  • [18] Tokatlı M., Identification of lactic acid bacteria isolated from pickles in Ankara Çubuk region, determination of their technological and functional properties and their potential for using as a starter culture, PhD thesis, University of Ankara, Ankara, Turkey, 2013.
  • [19] Yürümez E., Probiotics properties of some lactic acid bacteria isolated from faeces samples. MSc thesis, University of Ankara, Ankara, Turkey, 2011.
  • [20] Xu H., Jeong H.S., Lee H.Y. and Ahn J., Assessment of cell surface properties and adhesion potential of selected probiotic strains. Lett. Appl. Microbiol., 49 (2009) 434–442.
  • [21] Georgieva R., Yocheva L., Tserovska L., Zhelezova G., Stefanova N., Atanasova A., Danguleva A., Ivanova G., Karapetkov N., Rumyan N. and Karaivanova E., Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures, Biotechnol. Biotechnol. Equip., 29 (2015) 84–91.
  • [22] Zheng M., Zhang R., Tian X., Zhou X., Pan X. and Wong A., Assessing the risk of probiotic dietary supplements in the context of antibiotic resistance, Front. Microbiol., 8 (2017) 908. doi: 10.3389/fmicb.2017.00908
  • [23] Danielsen M. and Wind A., Susceptibility of Lactobacillus spp. to antimicrobial agents, Int. J. Food Microbiol., 82 (2003) 1-11.
  • [24] Berardi C. W., Solovey E.T. and Cummings M.L., Investigating the efficacy of network visualizations for intelligence tasks, in Proceedings of the IEEE International Conference on Intelligence and Security Informatics (Seattle, WA), (2013) 278–283.
  • [25] Plessas S., Nouska C., Karapetsas A., Kazakos S., Alexopoulos A., Mantzourani I., Chondrou P., Fournomiti M., Galanis A. and Bezirtzoglou E., Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese, Food Chem., 226 (2017) 102–108.
  • [26] Singroha G., Mishra S.K. and Malik R.K., Isolation and characterization of potential probiotic Lactobacillus gasseri strains isolated from different sources, Int. J. Food. Ferment., 6 (2017) 71-83.
  • [27] Oh N.S., Joung J.Y., Lee J.Y. and Kim Y., Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces. PLoSONE, 13(2) (2018) e0192021. https://doi.org/10.1371/journal.pone.0192021
  • [28] Argyri A.A.,Zoumpopoulou G., Karatzas K.A., Tsakalidou E., Nychas G.J., Panagou E.Z. and Tassou C.C., Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests, Food Microbiol., 33 (2013) 282-291.
  • [29] Ferreira C.L., Grześkowiak L., Collado M.C. and Salminen S., In vitro evaluation of Lactobacillus gasseri strains of infant origin on adhesion and aggregation of specific pathogens, J. Food Prot., 74 (2011) 1482–1487.
  • [30] Twetman L., Larsen U., Fiehn N.-E., Steckse ́n-Blicks C. and Twetman S., Coaggregation between probiotic bacteria and caries-associated strains: An in vitro study, Acta Odontol. Scand., 67 (2009) 284-288.
  • [31] Ocaña V.S. and Nader-Macias M.E., Vaginal lactobacilli: Self- and co-aggregating ability, Br. J. Biomed. Sci., 59 (2002) 183–190.
There are 31 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Meltem Aşan Özüsağlam 0000-0002-3638-1306

Ayşe Günyaktı 0000-0001-9301-8928

Submission Date December 2, 2019
Acceptance Date February 3, 2020
Publication Date March 22, 2020
DOI https://doi.org/10.17776/csj.654353
IZ https://izlik.org/JA83JS72LG
Published in Issue Year 2020 Volume: 41 Issue: 1

Cite

APA Aşan Özüsağlam, M., & Günyaktı, A. (2020). Investigation of lactobacillus gasseri ma-2 as probiotic candidate. Cumhuriyet Science Journal, 41(1), 228-234. https://doi.org/10.17776/csj.654353

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