Can an abts antioxidant test be performed without a spectrophotometer?

Zeynep Akar; Nesibe Arslan Burnaz

doi:10.17776/csj.642223

EN

Can an abts antioxidant test be performed without a spectrophotometer?

Abstract

ABTS^{• +} (2,2-azinobis- (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging assay is widely used to determine the antioxidant activity of natural and synthetic substances. As other antioxidant activity determination methods, a spectrophotometer device is needed to determine the numerical value of the color formed in ABTS method. Therefore, the dependence on the device is disadvantageous due to especially the high cost and restriction of workspace. To overcome this disadvantage, a new colorimetric method in the determination of antioxidant activity for ABTS method was developed with the help of a scanner device and free software (Image J). Mixtures (plant extracts and antioxidant standards) in this new colorimetric method were prepared as in the spectrophotometric method and, after the incubation period, each of these reagent mixtures was dropped onto the thin layer chromatography (TLC) plate. Then the colors that appeared on the thin layer chromatography were transferred to the computer with the help of a scanner and CSC50 values (the color value of the antioxidant concentration required to scavenge 50% of the ABTS radical in the test solution using Image J software) were measured with free software of Image J. The same measurements were performed simultaneously on the spectrophotometer. The results of both methods were compared. There was a strong correlation between the new colorimetric method and the spectrophotometric method for ABTS. Thus, the new colorimetric method for the ABTS test has become easily applicable since no spectrophotometer device is needed and in all environments.

Keywords

ABTS,Antioxidant,Colorimetric,Thin layer chromatography

Supporting Institution

Gümüşhane Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

15.F5119.02.01

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Zeynep Akar ^*
0000-0001-9262-8070
Türkiye

Nesibe Arslan Burnaz
0000-0003-1163-4829
Türkiye

Publication Date

March 22, 2020

Submission Date

November 4, 2019

Acceptance Date

January 5, 2020

Published in Issue

Year 2020 Volume: 41 Number: 1

DOI

https://doi.org/10.17776/csj.642223

IZ

https://izlik.org/JA95DU38SS

Cite

RIS / Bibtex

APA

Akar, Z., & Arslan Burnaz, N. (2020). Can an abts antioxidant test be performed without a spectrophotometer? Cumhuriyet Science Journal, 41(1), 185-192. https://doi.org/10.17776/csj.642223

Cited By

Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice

Rice Science

https://doi.org/10.1016/j.rsci.2024.10.004

Öz

Can an abts antioxidant test be performed without a spectrophotometer?

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice