Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

Ecehan Efe; Emine Yalçın; Kültiğin Çavuşoğlu

doi:10.17776/csj.513469

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Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

Abstract

In this study, antimutagenic, antimicrobial and antioxidant activities of Smilax excelsa L. fruit extract were evaluated. The antimicrobial effect was investigated by disk diffusion method and the antimutagenic effect was investigated by Ames/Salmonella/microsom test. The antioxidant properties of S. excelsa fruit samples were determined by investigating the total phenolic, flavonoid contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. It was observed that 200 mg plate^-1 fruit extract was found to have a mutagenicity inhibition as 81% in the absence of S9 mixture and 67% in the presence of S9 mixture against Salmonella typhimurium TA1535. S. excelsa fruit extract produced an inhibition zone in the range of 11-16 cm against the tested microorganisms. Flavonoid and phenolic contents were found to be 0.7985±0.0124 mgQE 100 mL^-1 and 11.9847±0.0041 mgGAE 100 mL^-1 at the 200 mg mL^-1 extract concentration, respectively. The DPPH removal rate was determined to be 55% at the 200 mg L^-1 dose. As a result, it was observed that the S. excelsa fruit tissues exhibited a highly antimutagenic activity and has been determined as a potential natural antimicrobial and antioxidant source.

Keywords

Ames/Salmonella/microsomal test,antioxidant activity,disc diffusion test

References

Stein C.M., Are herbal products dietary supplements or drugs? An important question for public safety, Clin. Pharmacol. Ther., 71-6 (2002) 411-413.
Süzer Ö., Serotonin, Serotonin Reseptörlerinin Baslica Etki Yerleri Ve Fonksiyonlari. Süzer Farmakoloji. 3. baskı. Istanbul: Klinisyen Tip Kitabevleri, (2005); pp150-154.
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Address: https://monographs.iarc.fr/wp-content/uploads/2018/06/mono94-4.pdf. Retrieved January 9, 2019.
Navarro M., Montilla M.P., Cabo M.M., Galisteo M., Cáceres A., Morales C. and Berger I., Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases, Phytother. Res., 1 (2003) 325–329.
Lee S.E., Ju E.M. and Kim J.H., Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root, Exp. Mol. Med., 33 (2001) 263-268.
Jiang J. and Xu Q., Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats, J. Ethnopharmacol., 85 (2003) 53-59.
Ozbucak T.B., Ergen A.O. and Yalcin S., Nutrition contents of the some wild edible plants in Central Black Sea region of Turkey, IJNES, 1 (2007) 11-13.
Ozsoy N., Can A., Yanardag R. and Akev N., Antioxidant activity of Smilax excelsa L. leaf extracts, Food Chem., 110 (2008) 571-583.

Ozsoy N., Okyar A., Arda-Pirincci P., Can A., Bolkent S. and Akev N., Evaluation of Smilax excelsa L. use in experimentally induced nephrotoxicity, J. Fac. Vet. Med. Kafkas University, 19-5 (2013) 807-814.
Efe E. Smilax excelsa L. meyve ekstrelerinin antimutajenik, antimikrobiyal ve antioksidan aktivitelerinin araştırılması. Giresun University Graduate School of Natural and Applied Sciences, Deparment of Biology, Master Thesiss. 16 (2017).
Cooper D.G. and Paddock P.A., Production of a biosurfactant from Torulopsis bombicola, Appl. Environ. Microbiol., 47-1 (1984) 173-176.
Singleton V.L. and Rossi J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Viticult., 16 (1965) 144-158.
Zhishen J., Mengcheng T. and Jianming W., The determination of flavonoid contents on mulberry and their scavenging effects on superoxide radical, Food Chem., 64 (1999) 555-559.
Yen G.C. and Duh P.D., Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species, J. Agric. Food Chem., 42 (1994) 629-632.
Maron D.M. and Ames B.N., Revised methods for the mutagenicity test, Mutat. Res., 113(1983) 173-215.
Uysal A., Zengin G., Durak Y. and Aktumsek A., Screening for antioxidant and antimutagenic properties of extracts from Centaurea pterocaula as well as their enzyme inhibitory potentials, Marmara Pharm. J., 20-3 (2016) 232-242.
Khaligh P., Salehi P., Farimani M.M., Ali-Asgari S., Esmaeili M.A. and Ebrahimi S.M., Bioactive compounds from Smilax excelsa L, J. Iran Chem. Soc., 13 (2016) 1055-1059.
Ivanova A., Mikhova B., Kostova I. and Evstatieva L., Bioactive chemical constituents from Smilax excels, Chem. Nat. Compd., 46 (2010) 295-297.
Schultes R.E., The Kingdom of Plants. In: Thomson WAR., editor. Medicines from the earth. New York: McGraw-Hill Book Co, 1978; pp 208.
Feng Q.L., Wu J., Chen G.Q., Cui F., Kim T.N., and Kim J.O., A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, J. Biomed. Mate. Res., 52-4 (2000) 662-668.
Lu C.L., Zhu W., Wang M., Xu X.J. and Lu C.J., Antioxidant and anti-inflammatory activities of phenolic-enriched extracts of Smilax glabra, J. Evid. Based Complement Altern. Med., 2014 (2014) 1-8.
Shuo X., Ming-Ying S., Guang-Xue L., Feng X., Xuan W., Cheng-Chao S. and Shao-Qing C., Chemical Constituents from the Rhizomes of Smilax glabra and their antimicrobial activity, Molecules, 18 (2013) 5265-5287.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Ecehan Efe
0000-0002-8843-051X
Türkiye

Emine Yalçın ^*
0000-0002-5280-5375
Türkiye

Kültiğin Çavuşoğlu
0000-0002-4767-9132
Türkiye

Publication Date

June 30, 2019

Submission Date

January 16, 2019

Acceptance Date

April 25, 2019

Published in Issue

Year 2019 Volume: 40 Number: 2

DOI

https://doi.org/10.17776/csj.513469

IZ

https://izlik.org/JA53EU75TT

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RIS / Bibtex

APA

Efe, E., Yalçın, E., & Çavuşoğlu, K. (2019). Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract. Cumhuriyet Science Journal, 40(2), 440-446. https://doi.org/10.17776/csj.513469

Determination of the Phytochemical, Antioxidant, and Antimicrobial Properties of $Smilax$ $excelsa$ L. Extracted with Different Solvents

Sinop Üniversitesi Fen Bilimleri Dergisi

https://doi.org/10.33484/sinopfbd.1587996

Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

Smilax excelsa L. Meyve Ekstresinin Antimutagenik ve Multi-Biyolojik aktiviteleri

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Anahtar Kelimeler

Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

Abstract

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References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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