Purification and characterization glutathione S-transferase enzyme from quail (Coturnix, coturnix japonica) heart and investigation the effect of some metal ions on enzyme activity

Barzan Mirza Ahmed; Yusuf Temel; Mehmet Çiftci

doi:10.17776/csj.496122

EN

Purification and characterization glutathione S-transferase enzyme from quail (Coturnix, coturnix japonica) heart and investigation the effect of some metal ions on enzyme activity

Abstract

In this study glutathione S-transferase enzyme (EC: 2.5.1.18) from the heart of japonica quail was purified with 34.0 EU/mg specific activity, 10.44% purification yield and 78.29 purification folds and characterized. Purification processes are consist of three steps, firstly homogenate was prepared, and then ammonium sulfate precipitation was performed and finally glutathione-agarose gel affinity column chromatography was performed. To check the purity of GST enzyme used SDS-PAGE method. Then the M.W calculated at approximately 26.3 kDa by SDS-PAGE method. Enzymatic activity was determined spectrofotometrically according to Beutler`s method at 340 nm. Also characterizations study carry out, and the results obtained are stability-pH = 9.0 in Tris/HCL buffer, optimum pH = 8.0 in Tris/HCl buffer, optimum temperature 60 °C, optimum ionic strength was 1.2 M in Tris/HCl buffer. And kinetic studies performed for GST enzyme purified from quail heart by used both glutathione and 1-chloro 2,4-dinitrobenzen as substrate. K_M and V_max values are determined as 1.642 mM and 0.502 EU/mL respectively for GSH substrate and 3.880 mM and 0.588 EU/mL respectively for CDNB substrate. In addition, the effect of some metal ions (Cu²⁺, Cd²⁺, Fe²⁺, Fe³⁺ Zn²⁺, Ag⁺, Co²⁺, and Ti¹⁺) were investigated on the GST enzyme activity in vitro.

Keywords

Quail,Heart,Glutathione S-transferase,Purification,Characterizations,,Metal Ions.

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Barzan Mirza Ahmed
Türkiye

Yusuf Temel
0000-0001-8148-3718
Türkiye

Mehmet Çiftci ^*

Publication Date

December 31, 2019

Submission Date

December 12, 2018

Acceptance Date

May 13, 2019

Published in Issue

Year 2019 Volume: 40 Number: 4

DOI

https://doi.org/10.17776/csj.496122

IZ

https://izlik.org/JA79YG47SH

Cite

RIS / Bibtex

APA

Mirza Ahmed, B., Temel, Y., & Çiftci, M. (2019). Purification and characterization glutathione S-transferase enzyme from quail (Coturnix, coturnix japonica) heart and investigation the effect of some metal ions on enzyme activity. Cumhuriyet Science Journal, 40(4), 802-812. https://doi.org/10.17776/csj.496122