Antidiabetic Potential: Effect of Resorcinol on α-Glycosidase and α-Amylase Enzymes

Fevzi Topal

doi:10.17776/csj.452514

EN TR

Antidiabetic Potential: Effect of Resorcinol on α-Glycosidase and α-Amylase Enzymes

Abstract

The vast majority of the carbohydrates in the biomolecule class are formed by photosynthesis using sunlight. One of the important enzymes in this class is α-glycosidase. This enzyme converts complex carbohydrates to monosaccharides. On the other hand, another important enzyme species is the α-amylase enzyme. Which directly related to starch. Starch is a type of polymer formed by the oxygen binding of glucose units to each other at the C1 position with glycosidic bonds. α-Amylase is an enzyme that catalyzes the hydrolysis of glycosidic bonds in starch, and this enzyme is purified from a wide variety of animals, plants, and microorganisms. Resorcinol, a phenol derivative, is an important organic chemical substance in the production of textile, medicine, hair dye, plastic and agricultural products of many industrial materials. Resorcinol, a phenol derivative, is present as white crystals, and resorcinol consists of two hydroxyl molecules attached to the benzene ring, and also was defined as α-glycosidase and α-amylase inhibitor to detect antidiabetic properties. Resorcinol was used as 𝛼-glycosidase and 𝛼-amylase inhibitor. Accordingly, IC₅₀values of α-amylase and α-glycosidase were calculated as 1.34 μM and 77.00 nM, respectively. On the other hand, K_ivalue for α-glycosidase was found as 30.16 nM. It has been observed that α-amylase and α-glycosidase enzymes can inhibit by resorcinol compound. It has the properties of inhibiting carbohydrate absorption by inhibiting α-glycosidase enzymes in the small intestine. For both metabolic enzymes, resorcinol may be preferred as an inhibitor. In this case, resorcinol may be used as an inhibitor against to healty problems like Diabetes mellitus and antiobesity.

Keywords

Resorcinol,𝛼-Glycosidase,𝛼-Amylase,Enzyme inhibition,Antidiabetic

Antidiyabetik Potansiyel: Resorsinol’ün α-Glukozidaz ve α-Amilaz Enzimleri Üzerine Etkisi

Öz

Biyomolekül sınıfında bulunan karbohidratların büyük çoğunluğu güneş ışığını kullanarak fotosentez sonucu oluşurlar. Bu sınıfın önemli enzimlerinden birisi ise 𝛼-Glikozidaz dır. Bu enzim, kompleks karbohidratları monosakkaritlere dönüştürür. Diğer taraftan bir başka önemli enzim türü ise α-amilaz enzimidir. Bu enzim ise nişasta ile doğrudan ilişkilidir. Nişasta, glukoz ünitelerinin birbirine glikozidik bağlarla C1 pozisyonunda yer alan oksijenden bağlanmasıyla oluşan bir polimer türüdür. α-Amilaz, nişastada bulunan bu glikozidik bağların hidrolizini katalizleyen enzimlerdir ve bu enzim çok çeşitli hayvan, bitki ve mikroorganizmalardan saflaştırılmaktadır. Bir fenol türevi olan resorsinol, birçok endüstriyel malzemelerin tekstil, ilaç, saç boyaları, plastik ve tarım ürünlerinin üretiminde önemli bir organik kimyasal maddedir. Fenol türevi olan resorsinol beyaz kristaller halinde bulunur ve resorsinol benzen halkasına bağlı iki hidroksil molekülünden oluşur. 𝛼-Glikozidaz ve 𝛼-amilaz inhibitörü olarak resorsinol kullanıldı. Buna göre α-amilaz ve α-glikosidaz, sırasıyla IC₅₀ değerleri 1,34 µM ve 77,00 nM olarak hesaplandı. Öte yandan, α-glikosidaz için K_i değeri 30,16 nM olarak bulundu. 𝛼-Amilaz ve 𝛼-glikozidaz enzimlerini resorsinol bileşiği inhibe edebildiği gözlemlendi. İnce barsakta 𝛼-glukozidaz enzimlerini inhibe ederek karbohidrat emilimini geciktirme özelliklerine sahiptirler. Her iki metabolik enzim için resorsinol, bir inhibitör olarak tercih edilebilir. Bu durumda resorsinol, Diyabet mellitus ve antiobezite gibi sağlık sorunlarına karşı inhibitör olarak kullanılabilir.

Anahtar Kelimeler

Resorsinol,𝛼-Glikozidaz,𝛼-Amilaz,Enzim Inhibisyon,Antidiyabetik

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Fevzi Topal ^*
Türkiye

Publication Date

December 24, 2018

Submission Date

August 9, 2018

Acceptance Date

November 2, 2018

Published in Issue

Year 2018 Volume: 39 Number: 4

DOI

https://doi.org/10.17776/csj.452514

IZ

https://izlik.org/JA25LE85YB

Cite

RIS / Bibtex

APA

Topal, F. (2018). Antidiabetic Potential: Effect of Resorcinol on α-Glycosidase and α-Amylase Enzymes. Cumhuriyet Science Journal, 39(4), 828-832. https://doi.org/10.17776/csj.452514

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