Research Article

Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode

Volume: 39 Number: 4 December 24, 2018
EN TR

Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode

Abstract

Herein, electrochemical behavior of cholecalciferol on a multiwalled carbon nanotube modified glassy carbon electrode was evaluated by using cyclic voltammetry. Voltammetric determination of cholecalciferol on the prepared modified electrode was carried out using linear sweep voltammetry. A good linearity was obtained with a correlation coefficient of 0.9914 between 5×10-5 – 1×10-3 M. The LOD and LOQ values were calculated as 1.7×10-5 and 5.1×10-5 M, respectively. The response of the proposed electrode was sufficiently repeatable for determining cholecalciferol. Finally, the proposed modified electrode was successfully applied to the determination of cholecalciferol in a commercial oral solution that contains 300.000 I.U cholecalciferol/mL. A simple liquid-liquid extraction technique by using methanol was followed to extract cholecalciferol from the oral liquid. The results obtained with the proposed method were in agreement with cholecalciferol content of the commercial oral solution.

Keywords

References

  1. [1] Højskov C.S., Heickendorff L., Møller H.J., High-Throughput Liquid–Liquid Extraction and LCMSMS Assay For Determination Of Circulating 25(OH) Vitamin D3 and D2 in the Routine Clinical Laboratory, Clin. Chim. Acta, 411 (2010) 114–116.
  2. [2] Kamankesh M., Mohammadi A., Mollahosseini A., Jazaeri S., Shahdoostkhany M., Vitamin D3 : Preconcentration and Determination in Cereal Samples Using Ultrasonic-Assisted Extraction and Microextraction Method, Cereal Chem. J., 94 (2017) 532–538.
  3. [3] Heaney R.P., Recker R.R., Grote J., Horst R.L., Armas L.A.G., Vitamin D3 Is More Potent Than Vitamin D2 in Humans, J. Clin. Endocrinol. Metab., 96 (2011) E447–E452.
  4. [4] Sklan D., Budowski P., Katz M., Determination of 25-Hydroxycholecalciferol by Combined Thin Layer and Gas Chromatography, Anal. Biochem., 56 (1973) 606–609.
  5. [5] Vanhaelen-fastré R., Vanhaelen M., High-Performance Liquid Chromatography Determination of Potential Content Of Vitamin D2 (Ergocalciferol) and Vitamin D3 (Cholecalciferol) in Resins, Oils, Dry Concentrates and Multivitamin Formulations, J. Chromatogr. A, 153 (1978) 219–226.
  6. [6] Takeuchi A., Okano T., Ayame M., Yoshikawa H., Teraoka S., Murakami Y., Kobayashi, T., High-Performance Liquid Chromatographic Determination of Vitamin D3 in Fish Liver Oils and Eel Body Oils., J. Nutr. Sci. Vitaminol., 30 (1984) 421–430.
  7. [7] Aksnes L., A Simplified High-Performance Liquid Chromatographic Method For Determination of Vitamin D3, 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Human Serum., Scand. J. Clin. Lab. Invest., 52 (1992) 177–182.
  8. [8] Mattila P., Piironen V., Bäckman C., Asunmaa A., Uusi-Rauva E., Koivistoinen P., Determination of Vitamin D3 in Egg Yolk by High-Performance Liquid Chromatography with Diode Array Detection, J. Food Compos. Anal., 5 (1992) 281–290.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Şerife Eryılmaz Kahya
Türkiye

Ali Çelik *
0000-0002-4676-1759
Türkiye

Publication Date

December 24, 2018

Submission Date

July 2, 2018

Acceptance Date

November 19, 2018

Published in Issue

Year 1970 Volume: 39 Number: 4

APA
Eryılmaz Kahya, Ş., Cittan, M., & Çelik, A. (2018). Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode. Cumhuriyet Science Journal, 39(4), 1081-1088. https://doi.org/10.17776/csj.439630

Cited By

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December